Integrated coherent Raman scattering and multiphoton microscopy for label-free imaging of the dentin in the tooth

2014 ◽  
Author(s):  
Zi Wang ◽  
Wei Zheng ◽  
Jian Lin ◽  
Chin-Ying Hsu ◽  
Zhiwei Huang
Keyword(s):  
Raman Scattering ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Daniel Jaeger ◽  
Christian Pilger ◽  
Henning Hachmeister ◽  
Elina Oberländer ◽  
Robin Wördenweber ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Label Free ◽  
Intracellular Lipid ◽  
Coherent Raman Scattering ◽  
In Vivo Analysis ◽  
Coherent Raman

scholarly journals Dissecting lipid droplet biology with coherent Raman scattering microscopy

2021 ◽  
Vol 135 (5) ◽  
Author(s):  
Tao Chen ◽  
Ahmet Yavuz ◽  
Meng C. Wang
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Quantitative Imaging ◽  
Live Cells ◽  
Label Free ◽  
Genetic Screens ◽  
Spatiotemporal Resolution ◽  
Coherent Raman Scattering ◽  
Coherent Raman

ABSTRACT Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, composition and mobility of LDs are crucial for their physiological and pathological functions, and these different parameters of LDs are subject to regulation by genetic factors and environmental inputs. Coherent Raman scattering (CRS) microscopy utilizes optical nonlinear processes to probe the intrinsic chemical bond vibration, offering label-free, quantitative imaging of lipids in vivo with high chemical specificity and spatiotemporal resolution. In this Review, we provide an overview over the principle of CRS microscopy and its application in tracking different parameters of LDs in live cells and organisms. We also discuss the use of CRS microscopy in genetic screens to discover lipid regulatory mechanisms and in understanding disease-related lipid pathology.

scholarly journals Quantitative Label-Free Imaging of Lipid Domains in Single Bilayers by Hyperspectral Coherent Raman Scattering

2020 ◽  
Vol 92 (21) ◽  
pp. 14657-14666
Author(s):  
Alexander Nahmad-Rohen ◽  
David Regan ◽  
Francesco Masia ◽  
Craig McPhee ◽  
Iestyn Pope ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Lipid Domains ◽  
Label Free ◽  
Coherent Raman Scattering ◽  
Coherent Raman

Coherent Raman scattering microscopy for label-free imaging of live amphioxus

2012 ◽  
Author(s):  
Zhilong Yu ◽  
Tao Chen ◽  
Xiannian Zhang ◽  
Jie Shen ◽  
Junyuan Chen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Label Free ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals Broadband Coherent Raman Scattering Microscopy

2018 ◽  
Vol 12 (9) ◽  
pp. 1800020 ◽  
Author(s):  
Dario Polli ◽  
Vikas Kumar ◽  
Carlo M. Valensise ◽  
Marco Marangoni ◽  
Giulio Cerullo
Keyword(s):  
Raman Scattering ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview

2011 ◽  
Vol 16 (2) ◽  
pp. 021106 ◽  
Author(s):  
Sophie Brustlein ◽  
Patrick Ferrand ◽  
Nico Walther ◽  
Sophie Brasselet ◽  
Cyrille Billaudeau ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Light Source ◽  
Optical Parametric Oscillator ◽  
Parametric Oscillator ◽  
Optical Parametric ◽  
Coherent Raman Scattering ◽  
Coherent Raman
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