scholarly journals Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Daniel Jaeger ◽  
Christian Pilger ◽  
Henning Hachmeister ◽  
Elina Oberländer ◽  
Robin Wördenweber ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Label Free ◽  
Intracellular Lipid ◽  
Coherent Raman Scattering ◽  
In Vivo Analysis ◽  
Coherent Raman

scholarly journals Dissecting lipid droplet biology with coherent Raman scattering microscopy

2021 ◽  
Vol 135 (5) ◽  
Author(s):  
Tao Chen ◽  
Ahmet Yavuz ◽  
Meng C. Wang
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Quantitative Imaging ◽  
Live Cells ◽  
Label Free ◽  
Genetic Screens ◽  
Spatiotemporal Resolution ◽  
Coherent Raman Scattering ◽  
Coherent Raman

ABSTRACT Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, composition and mobility of LDs are crucial for their physiological and pathological functions, and these different parameters of LDs are subject to regulation by genetic factors and environmental inputs. Coherent Raman scattering (CRS) microscopy utilizes optical nonlinear processes to probe the intrinsic chemical bond vibration, offering label-free, quantitative imaging of lipids in vivo with high chemical specificity and spatiotemporal resolution. In this Review, we provide an overview over the principle of CRS microscopy and its application in tracking different parameters of LDs in live cells and organisms. We also discuss the use of CRS microscopy in genetic screens to discover lipid regulatory mechanisms and in understanding disease-related lipid pathology.

scholarly journals Coherent Raman Scattering Microscopy in Oncology Pharmacokinetic Research

2021 ◽  
Vol 12 ◽  
Author(s):  
Junjie Zeng ◽  
Wenying Zhao ◽  
Shuhua Yue
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Cancer Drug ◽  
Cancer Drugs ◽  
High Speed Imaging ◽  
Coherent Raman Scattering ◽  
Anti Cancer ◽  
Coherent Raman

The high attrition rates of anti-cancer drugs during clinical development remains a bottleneck problem in pharmaceutical industry. This is partially due to the lack of quantitative, selective, and rapid readouts of anti-cancer drug activity in situ with high resolution. Although fluorescence microscopy has been commonly used in oncology pharmacological research, fluorescent labels are often too large in size for small drug molecules, and thus may disturb the function or metabolism of these molecules. Such challenge can be overcome by coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, high spatial resolution, and high-speed imaging, without the need of any labeling. Coherent Raman scattering microscopy has tremendously improved the understanding of pharmaceutical materials in the solid state, pharmacokinetics of anti-cancer drugs and nanocarriers in vitro and in vivo. This review focuses on the latest applications of coherent Raman scattering microscopy as a new emerging platform to facilitate oncology pharmacokinetic research.

scholarly journals Quantitative Label-Free Imaging of Lipid Domains in Single Bilayers by Hyperspectral Coherent Raman Scattering

2020 ◽  
Vol 92 (21) ◽  
pp. 14657-14666
Author(s):  
Alexander Nahmad-Rohen ◽  
David Regan ◽  
Francesco Masia ◽  
Craig McPhee ◽  
Iestyn Pope ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Lipid Domains ◽  
Label Free ◽  
Coherent Raman Scattering ◽  
Coherent Raman

Coherent Raman scattering microscopy for label-free imaging of live amphioxus

2012 ◽  
Author(s):  
Zhilong Yu ◽  
Tao Chen ◽  
Xiannian Zhang ◽  
Jie Shen ◽  
Junyuan Chen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Label Free ◽  
Coherent Raman Scattering ◽  
Coherent Raman

scholarly journals Broadband Coherent Raman Scattering Microscopy

2018 ◽  
Vol 12 (9) ◽  
pp. 1800020 ◽  
Author(s):  
Dario Polli ◽  
Vikas Kumar ◽  
Carlo M. Valensise ◽  
Marco Marangoni ◽  
Giulio Cerullo
Keyword(s):  
Raman Scattering ◽  
Coherent Raman Scattering ◽  
Coherent Raman
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