Assembly and characterization of a nonlinear optical microscopy forin vivoandex vivotissue imaging

2014 ◽  
Author(s):  
S. Pratavieira ◽  
H. H. Buzzá ◽  
A. E. Jorge ◽  
C. Grecco ◽  
L. Pires ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy

Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy

2021 ◽  
pp. 1-11
Author(s):  
Chao-Wei Hung ◽  
Nirmal Mazumder ◽  
Dan-Jae Lin ◽  
Wei-Liang Chen ◽  
Shih-Ting Lin ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Label Free ◽  
Nonlinear Optical Microscopy ◽  
Collagen Crosslinking ◽  
Engineered Materials

Abstract

scholarly journals In situ 3D characterization of historical coatings and wood using multimodal nonlinear optical microscopy

2012 ◽  
Vol 20 (22) ◽  
pp. 24623 ◽  
Author(s):  
Gaël Latour ◽  
Jean-Philippe Echard ◽  
Marie Didier ◽  
Marie-Claire Schanne-Klein
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy

Characterization of human arterial tissue affected by atherosclerosis using multimodal nonlinear optical microscopy

2016 ◽  
Author(s):  
Enrico Baria ◽  
Riccardo Cicchi ◽  
Matteo Rotellini ◽  
Gabriella Nesi ◽  
Daniela Massi ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy ◽  
Arterial Tissue

scholarly journals Characterization of a synthetic bioactive polymer by nonlinear optical microscopy

2013 ◽  
Vol 5 (1) ◽  
pp. 149 ◽  
Author(s):  
N. Djaker ◽  
S. Brustlein ◽  
G. Rohman ◽  
S. Huot ◽  
M. Lamy de la Chapelle ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy

scholarly journals Scanning He Ion- and Nonlinear Optical Microscopy Combined with Force Measurements for the Characterization of Spider Silk

2017 ◽  
Vol 112 (3) ◽  
pp. 593a
Author(s):  
Irina Iachina ◽  
Jacek Fiutowski ◽  
Serguei Chiriaev ◽  
Per Lyngs Hansen ◽  
Adam Cohen Simonsen ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Spider Silk ◽  
Force Measurements ◽  
Nonlinear Optical Microscopy

scholarly journals Characterization of medieval-like glass alteration layers by laser spectroscopy and nonlinear optical microscopy

2021 ◽  
Vol 136 (8) ◽  
Author(s):  
Mohamed Oujja ◽  
Teresa Palomar ◽  
Marina Martínez-Weinbaum ◽  
Sagrario Martínez-Ramírez ◽  
Marta Castillejo
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Three Dimensional ◽  
Chemical Characterization ◽  
Conservation Strategies ◽  
Cross Sectional ◽  
Nonlinear Optical Microscopy ◽  
Non Destructive ◽  
Ft Raman

AbstractHistorical glass-based objects undergo, since the time of their manufacture, different degradation phenomena that are related to their composition and to the environment to which they were exposed. Three-dimensional (3D) structural and chemical characterization of the degradation layers is important to select the most adequate conservation strategies for glass objects. Optical microscopy (OM) is the most frequently used non-destructive method to examine the surface of historical glasses; however, the 3D structural assessment of alteration layers requires applying the destructive modality of this technique to conduct a cross-sectional study. In this work, a different approach for structural and compositional characterization of alteration layers on model medieval-like glasses is presented, based on the combination of the laser spectroscopies of laser-induced breakdown spectroscopy (LIBS), laser-induced fluorescence (LIF) and FT-Raman, and the emerging, cutting edge technique of nonlinear optical microscopy (NLOM) in the modality of multiphoton excitation fluorescence (MPEF). The results obtained through this multi-analytical photonic approach were compared with those retrieved by examination of the surface and cross sections of the samples by OM and scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM–EDS). While the combination of LIBS, LIF and FT-Raman served to assess the composition of the various alteration layers, the use of MPEF microscopy allowed the non-destructive determination of the thicknesses of these layers, showing for both thickness and composition a good agreement with the OM and SEM–EDS results. Thus, the proposed approach, which avoids sample preparation, illustrates the capability of non-destructive, or micro-destructive in the case of LIBS, laser spectroscopies and microscopies for the in situ study of glass objects of historic or/and artistic value. Graphic Abstract

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