Separation of biological cells in a microfluidic device using surface acoustic waves (SAWs)

2014 ◽  
Author(s):  
Ye Ai ◽  
Babetta L. Marrone
Keyword(s):  
Microfluidic Device ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Biological Cells

Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels

Lab on a Chip ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 4064-4070 ◽  
Author(s):  
Haiwei Lu ◽  
Kirk Mutafopulos ◽  
John A. Heyman ◽  
Pascal Spink ◽  
Liang Shen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Acoustic Waves ◽  
High Efficiency ◽  
Surface Acoustic Waves ◽  
Microfluidic Channels ◽  
Wide Range

We introduce a microfluidic device that uses traveling surface acoustic waves to lyse bacteria with high efficiency. This lysis method should be applicable to a wide range of bacteria species and can be modified to analyze individual bacteria cells.

scholarly journals Selective cell encapsulation, lysis, pico-injection and size-controlled droplet generation using traveling surface acoustic waves in a microfluidic device

Lab on a Chip ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 3914-3921
Author(s):  
Kirk Mutafopulos ◽  
Peter J. Lu ◽  
Ryan Garry ◽  
Pascal Spink ◽  
David A. Weitz
Keyword(s):  
Microfluidic Device ◽  
Acoustic Waves ◽  
Single Cells ◽  
Surface Acoustic Waves ◽  
Cell Encapsulation ◽  
Droplet Generation ◽  
New Materials ◽  
On Demand ◽  
Interdigital Transducer ◽  
Size Controlled

We generate traveling surface acoustic waves with an interdigital transducer to create droplets on-demand; encapsulate single cells; lyse cells and immediately encapsulate their contents; and pico-inject new materials into existing droplets.

O-124 Contact-free oocyte denudation in a chip-scale ultrasonic microfluidic device

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Mokhtare ◽  
P Xie ◽  
B Davaji ◽  
A Abbaspourrad ◽  
Z Rosenwaks ◽  
...  
Keyword(s):  
Mechanical Stress ◽  
Embryo Development ◽  
Microfluidic Device ◽  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Gas Permeability ◽  
Surface Acoustic Waves ◽  
Radiation Force ◽  
Ultrasonic Waves ◽  
Acoustic Radiation Force

Abstract Study question To design and test an automated microfluidic device to revolutionize the cumulus-oocyte-complex (COC) denudation procedure for intracytoplasmic sperm injection (ICSI) using murine oocytes. Summary answer Oocyte exposure to temperature variation, mechanical stress, and prolonged chemical treatment during denudation was mitigated using our microfluidic device based on surface acoustic waves (SAWs). What is known already COC denudation is a prerequisite for many ART procedures such as ICSI. However, this procedure is based on manual pipetting (MP), which lacks standardization and requires experienced embryologists to perform. Inadequate MP may damage oocytes through prolonged enzymatic treatment or high fluidic stresses and may jeopardize gamete competence. The use of microfluidic devices based on porous membranes or microchannels has been adopted by many laboratories for sperm selection. Of these, microchannel devices may also be adapted for denudation with minimal mechanical stress in a controlled microenvironment. However, oocyte manipulation and extraction have proven difficult to achieve. Study design, size, duration We developed a novel ultrasonic microfluidic device based on a microwell design manufactured with Polydimethylsiloxane (PDMS). The SAWs were generated by 4 interdigitated transducers (IDTs) arranged in an orthogonally symmetric pattern. A non-toxic dosage of ultrasonic waves, similar to those used in gynecology and obstetrics, was applied. COCs were denuded by induced acoustic streaming and acoustic radiation force. Denudation rate, embryo development, and pregnancy outcomes were assessed and compared to control oocytes denuded by MP. Participants/materials, setting, methods For each run, up to 10 individual COCs from super-ovulated B6D2F1 mice were loaded into the microwell alongside diluted hyaluronidase (20 IU/ml) and denuded by 80 or 200 MHz SAWs. Denuded oocytes were fertilized by piezo-actuated ICSI using spermatozoa from the same strain. Pre-implantation embryo development was assessed in a time-lapse incubator for up to 96 h. High-quality blastocysts were transferred to 2.5-dpc pseudo-pregnant CD-1 surrogates. Pregnancy and offspring health were observed. Main results and the role of chance Using alternating frequency sweep in a pulse-repetition mode, we swirled the fluid inside the microwell consistently and tumbled COCs inside the microwell to expose them to acoustic steaming-induced drag forces and acoustic radiation force. Using a high-speed camera and particle-tracking technique, we observed that the drag force generated by the SAWs fulfilled the denudation mechanism. Additionally, due to the small attenuation coefficient in water, thermal absorption heating remains minuscule, preventing any thermal-induced damage. Our device significantly reduced the time and labor of the denudation process. It also yielded proper denudation quality without oocyte loss. To ensure that SAWs do not damage oocytes, 40 oocytes denuded by 80 MHz SAWs, 25 oocytes denuded by 200 MHz SAWs, and 30 oocytes denuded by MP were inseminated by piezo-actuated ICSI. The 80-MHz, 200-MHz, and MP groups yielded comparable post-ICSI survival (82.5% vs. 84.0% vs. 83.3%, respectively), fertilization (80.0% vs. 80.0% vs. 83.3%,respectively), and blastulation rates (72.5% vs. 82.0% vs. 66.7%, respectively). Embryo morphokinetics were also not impacted. After transferring all blastocysts into recipient mice, 8 live births were achieved from the 80-MHz group, while 5 were achieved from the 200-MHz group. Limitations, reasons for caution Although PDMS is a popular material due to its high optical transparency and biocompatibility, adverse effects due to gas permeability and small-molecule adsorption cannot be excluded. Large-scale mouse embryo assays should be performed to assess the teratogenicity of PDMS. Operation parameters must be optimized for human COCs in clinical application. Wider implications of the findings Adopting widely used ultrasound techniques with emerging SAW technology is a major step toward advancing and standardizing oocyte denudation—a laborious yet delicate procedure. We predict it will be further integrated with AI and miniaturized robotics, modules specialized in gamete assessment, ICSI, and embryo evaluation in the near future. Trial registration number ‘not applicable’

Sorting of tumour cells in a microfluidic device by multi-stage surface acoustic waves

2018 ◽  
Vol 258 ◽  
pp. 1174-1183 ◽  
Author(s):  
Kaiyue Wang ◽  
Wei Zhou ◽  
Zhengrong Lin ◽  
Feiyan Cai ◽  
Fei Li ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Tumour Cells ◽  
Multi Stage

Manipulation of microbubbles and targeted single cell sonoporation with by surface acoustic waves in a microfluidic device

2016 ◽  
Vol 140 (4) ◽  
pp. 3149-3149
Author(s):  
Long Meng ◽  
Feiyan Cai ◽  
Juanjuan Chen ◽  
Lili Niu ◽  
Hairong Zheng ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Acoustic Waves ◽  
Surface Acoustic Waves

scholarly journals Three-dimensional manipulation of single cells using surface acoustic waves

2016 ◽  
Vol 113 (6) ◽  
pp. 1522-1527 ◽  
Author(s):  
Feng Guo ◽  
Zhangming Mao ◽  
Yuchao Chen ◽  
Zhiwei Xie ◽  
James P. Lata ◽  
...  
Keyword(s):  
Acoustic Waves ◽  
Single Cells ◽  
Surface Acoustic Waves ◽  
Three Dimensional ◽  
Biological Cells ◽  
Acoustic Vibrations ◽  
Label Free ◽  
Acoustic Tweezers ◽  
Contact Free ◽  
Micrometer Scale

The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.

Characterization of RF Sputtered ZnO Piezoelectric Films Using Transmission Electron Microscope

1975 ◽  
Vol 33 ◽  
pp. 86-87
Author(s):  
Kemining W. Yeh ◽  
Richard S. Muller ◽  
Wei-Kuo Wu ◽  
Jack Washburn
Keyword(s):  
Integrated Circuit ◽  
Acoustic Waves ◽  
New Technology ◽  
Surface Acoustic Waves ◽  
Electromechanical Properties ◽  
Leading Role ◽  
Saw Devices ◽  
Transmission Electron ◽  
High Degree

Considerable and continuing interest has been shown in the thin film transducer fabrication for surface acoustic waves (SAW) in the past few years. Due to the high degree of miniaturization, compatibility with silicon integrated circuit technology, simplicity and ease of design, this new technology has played an important role in the design of new devices for communications and signal processing. Among the commonly used piezoelectric thin films, ZnO generally yields superior electromechanical properties and is expected to play a leading role in the development of SAW devices.

Attenuation of surface acoustic waves by quantum dots

1998 ◽  
Vol 77 (5) ◽  
pp. 1195-1202
Author(s):  
Andreas Knabchen Yehoshua, B. Levinson, Ora
Keyword(s):  
Quantum Dots ◽  
Acoustic Waves ◽  
Surface Acoustic Waves
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