Micro pore arrays in free standing cyclic olefin copolymer membranes: fabrication and surface functionalization strategies for in-vitro barrier tissue models

2013 ◽  
Author(s):  
M. Gel ◽  
S. Kandasamy ◽  
K. Cartledge ◽  
C. L. Be ◽  
D. Haylock
Keyword(s):  
Surface Functionalization ◽  
Cyclic Olefin Copolymer ◽  
Free Standing ◽  
Cyclic Olefin ◽  
Tissue Models

Fabrication of free standing microporous COC membranes optimized for in vitro barrier tissue models

2014 ◽  
Vol 215 ◽  
pp. 51-55 ◽  
Author(s):  
M. Gel ◽  
S. Kandasamy ◽  
K. Cartledge ◽  
D. Haylock
Keyword(s):  
Free Standing ◽  
Tissue Models

Surface functionalization of cyclic olefin copolymer with aryldiazonium salts: A covalent grafting method

2015 ◽  
Vol 329 ◽  
pp. 337-346 ◽  
Author(s):  
Florian Brisset ◽  
Julien Vieillard ◽  
Benjamin Berton ◽  
Sandrine Morin-Grognet ◽  
Cécile Duclairoir-Poc ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin ◽  
Covalent Grafting

Facile Surface Functionalization of Cyclic Olefin Copolymer Film with Anhydride Groups for Protein Microarray Fabrication

2020 ◽  
Vol 3 (5) ◽  
pp. 3203-3209
Author(s):  
Yuan Qi ◽  
Yindian Wang ◽  
Chuxuan Chen ◽  
Changwen Zhao ◽  
Yuhong Ma ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Protein Microarray ◽  
Copolymer Film ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin ◽  
Microarray Fabrication

scholarly journals Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

2021 ◽  
Author(s):  
Skadi Lau ◽  
Yue Liu ◽  
Anna Maier ◽  
Steffen Braune ◽  
Manfred Gossen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Endothelial Cell ◽  
Cell Layer ◽  
Test System ◽  
Cyclic Olefin Copolymer ◽  
Flow Conditions ◽  
Cyclic Olefin ◽  
Platelet Adherence ◽  
Endothelial Cell Layer

AbstractIn vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials. Graphic abstract

Surface functionalization of cyclic olefin copolymer (COC) with evaporated TiO2 thin film

2016 ◽  
Vol 363 ◽  
pp. 670-675 ◽  
Author(s):  
Lamia El Fissi ◽  
Denis Vandormael ◽  
Laurent Houssiau ◽  
Laurent A. Francis
Keyword(s):  
Thin Film ◽  
Surface Functionalization ◽  
Tio2 Thin Film ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin

Effect of the number of arms on the mechanical properties of a star-shaped cyclic olefin copolymer

2019 ◽  
Vol 10 (41) ◽  
pp. 5578-5583 ◽  
Author(s):  
Takumitsu Kida ◽  
Ryo Tanaka ◽  
Koh-hei Nitta ◽  
Takeshi Shiono
Keyword(s):  
Mechanical Properties ◽  
Thermal Properties ◽  
Physical Properties ◽  
Secondary Amine ◽  
Aggregation Number ◽  
Drastic Change ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin

The increase of aggregation number in a star-shaped cyclic olefin copolymer was succeeded by using a triazine-based secondary amine, which caused a drastic change in physical properties without changing the thermal properties.

scholarly journals Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 915
Author(s):  
Gözde Dursun ◽  
Muhammad Umer ◽  
Bernd Markert ◽  
Marcus Stoffel
Keyword(s):  
Mobile Application ◽  
Low Cost ◽  
Experimental Information ◽  
Raspberry Pi ◽  
Tissue Constructs ◽  
Cost Controlling ◽  
Controlling System ◽  
And Control ◽  
Tissue Models

(1) Background: Bioreactors mimic the natural environment of cells and tissues by providing a controlled micro-environment. However, their design is often expensive and complex. Herein, we have introduced the development of a low-cost compression bioreactor which enables the application of different mechanical stimulation regimes to in vitro tissue models and provides the information of applied stress and strain in real-time. (2) Methods: The compression bioreactor is designed using a mini-computer called Raspberry Pi, which is programmed to apply compressive deformation at various strains and frequencies, as well as to measure the force applied to the tissue constructs. Besides this, we have developed a mobile application connected to the bioreactor software to monitor, command, and control experiments via mobile devices. (3) Results: Cell viability results indicate that the newly designed compression bioreactor supports cell cultivation in a sterile environment without any contamination. The developed bioreactor software plots the experimental data of dynamic mechanical loading in a long-term manner, as well as stores them for further data processing. Following in vitro uniaxial compression conditioning of 3D in vitro cartilage models, chondrocyte cell migration was altered positively compared to static cultures. (4) Conclusion: The developed compression bioreactor can support the in vitro tissue model cultivation and monitor the experimental information with a low-cost controlling system and via mobile application. The highly customizable mold inside the cultivation chamber is a significant approach to solve the limited customization capability of the traditional bioreactors. Most importantly, the compression bioreactor prevents operator- and system-dependent variability between experiments by enabling a dynamic culture in a large volume for multiple numbers of in vitro tissue constructs.

Sign in / Sign up

Export Citation Format

Share Document