Label free optimal dynamic discrimination of biological macromolecules

2013 ◽  
Author(s):  
S. Afonina ◽  
A. Rondi ◽  
D. Kiselev ◽  
L. Bonacina ◽  
J. P. Wolf
Keyword(s):  
Biological Macromolecules ◽  
Label Free ◽  
Optimal Dynamic ◽  
Dynamic Discrimination

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

Nanomonitor Technology for Glycosylation Analysis

2009 ◽  
Vol 1236 ◽  
Author(s):  
Gaurav Chatterjee ◽  
Manish Bothara ◽  
Srivatsa Aithal ◽  
Vinay J Nagraj ◽  
Peter Wiktor ◽  
...  
Keyword(s):  
Double Layer ◽  
Electrical Double Layer ◽  
Printed Circuit Board ◽  
Electrochemical Impedance ◽  
Electrochemical Techniques ◽  
Protein Glycosylation ◽  
Circuit Board ◽  
Biological Macromolecules ◽  
Label Free ◽  
Alumina Membrane

AbstractChanges in protein glycosylation have great potential as markers for the early diagnosis of cancer and other diseases. The current analytical tools for the analysis of glycan structures need expensive instrumentation, advanced expertise, is time consuming and therefore not practical for routine screening of glycan biomarkers from human samples in a clinical setting.We are developing a novel ultrasensitive diagnostic platform called ‘NanoMonitor’ to enable rapid label-free glycosylation analysis. The technology is based on electrochemical impedance spectroscopy where capacitance changes are measured at the electrical double layer interface as a result of interaction of two molecules.The NanoMonitor platform consists of a printed circuit board with array of electrodes forming multiple sensor spots. Each sensor spot is overlaid with a nanoporous alumina membrane that forms a high density of nanowells. Lectins, proteins that bind to and recognize specific glycan structures, are conjugated to the surface of nanowells. When specific glycoproteins from a test sample bind to lectins in the nanowells, it produces a perturbation to the electrical double layer at the solid/liquid interface at the base of each nanowell. This perturbation results in a change in the impedance of the double layer which is dominated by the capacitance changes within the electrical double layer.The nanoscale confinement or crowding of biological macromolecules within the nanowells is likely to enhance signals from the interaction of glycoproteins with the lectins leading to a high sensitivity of detection with the NanoMonitor as compared to other electrochemical techniques.Using a panel of lectins, we were able to detect subtle changes in the glycosylation of fetuin protein as well as differentiate glycoproteins from normal versus cancerous cells. Our results indicate that NanoMonitor can be used as a cost-effective miniature electronic biosensor for the detection of glycan biomarkers.

Exploring the capabilities of quantum optimal dynamic discrimination

2009 ◽  
Vol 130 (16) ◽  
pp. 164112 ◽  
Author(s):  
Vincent Beltrani ◽  
Pritha Ghosh ◽  
Herschel Rabitz
Keyword(s):  
Optimal Dynamic ◽  
Dynamic Discrimination

scholarly journals Resolution of strongly competitive product channels with optimal dynamic discrimination: Application to flavins

2011 ◽  
Vol 134 (3) ◽  
pp. 034511 ◽  
Author(s):  
Jonathan Roslund ◽  
Matthias Roth ◽  
Laurent Guyon ◽  
Véronique Boutou ◽  
Francois Courvoisier ◽  
...  
Keyword(s):  
Optimal Dynamic ◽  
Dynamic Discrimination ◽  
Competitive Product

scholarly journals Optimal Dynamic Discrimination in Tryptophan-Containing Dipeptides

2013 ◽  
Vol 41 ◽  
pp. 07012
Author(s):  
S. Afonina ◽  
O. Nenadl ◽  
A. Rondi ◽  
D. Kiselev ◽  
J. Extermann ◽  
...  
Keyword(s):  
Optimal Dynamic ◽  
Dynamic Discrimination

Optimal Dynamic Discrimination of Similar Molecules through Quantum Learning Control†

2002 ◽  
Vol 106 (33) ◽  
pp. 8125-8131 ◽  
Author(s):  
Baiqing Li ◽  
Gabriel Turinici ◽  
Viswanath Ramakrishna ◽  
Herschel Rabitz
Keyword(s):  
Learning Control ◽  
Quantum Learning ◽  
Optimal Dynamic ◽  
Dynamic Discrimination

Optimal dynamic discrimination of similara quantum systems in the presence of decoherence

2006 ◽  
Vol 124 (2) ◽  
pp. 024101 ◽  
Author(s):  
Baiqing Li ◽  
Wusheng Zhu ◽  
Herschel Rabitz
Keyword(s):  
Quantum Systems ◽  
Optimal Dynamic ◽  
Dynamic Discrimination
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