Integrated on-chip lens applied to microfluidic chips

2012 ◽  
Author(s):  
Yingying Zhao ◽  
Qin Li ◽  
Xiao-Ming Hu ◽  
Dong-Fang Yang
Keyword(s):  
Microfluidic Chips ◽  
On Chip

Dynamic microfluidic nanocalorimetry system for measuring Caenorhabditis elegans metabolic heat

Lab on a Chip ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 1641-1651 ◽  
Author(s):  
Roger Krenger ◽  
Thomas Lehnert ◽  
Martin A. M. Gijs
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Chips ◽  
C Elegans ◽  
Metabolic Heat ◽  
On Chip

A nanocalorimetry system with integrated microfluidic chips for on-chip measurements of metabolic heat signals of C. elegans.

scholarly journals On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

AIP Advances ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 095213 ◽  
Author(s):  
Hiroaki Takehara ◽  
Osawa Kazutaka ◽  
Makito Haruta ◽  
Toshihiko Noda ◽  
Kiyotaka Sasagawa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Analysis ◽  
Microfluidic Chips ◽  
On Chip ◽  
Analysis Platform

An LED-induced fluorescence detection system with integrated on-chip lens based on microfluidic chips technology/Ein LED-induziertes Fluoreszenzdetektionssystem mit integrierter „Lens-on-Chip“ basierend auf der mikrofluidischen Chiptechnologie

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Yingying Zhao ◽  
Qin Li ◽  
Xiao-Ming Hu ◽  
Dong-Fang Yang
Keyword(s):  
Optical Fibers ◽  
Detection Efficiency ◽  
Detection System ◽  
Research Work ◽  
Coupling Efficiency ◽  
Optical Excitation ◽  
Optical Signal ◽  
Microfluidic Chips ◽  
Fluorescence Excitation ◽  
On Chip

AbstractThe power density of optical excitation on microfluidic chips is attenuated due to the beam divergence of the optical fiber, making it difficult to collect either the emission or scattering light. The excitation power and coupling efficiency can be significantly increased by using an on-chip microlens system with integrated optical fibers, specially designed and simulated to reshape and collect the optical signal within the microfluidic chip.This article presents research work for designing, fabricating, and testing a type of on-chip microlens. The on-chip microlens was designed by Code VThe export light beam could be focused to a very small point to satisfy the requirements of the fluorescence excitation of samples. On the other hand, the detection efficiency could also be increased with the help of the on-chip microlens. The presented fabrication method does not require any assembly process or external driving force. To fabricate a microlens with a different focus, it is only necessary to change the figures on the mask. This technique can be used in a variety of applications as it is possible to produce customized microlens for specific applications.

Fluorescence hyperspectral imaging for live monitoring of multiple spheroids in microfluidic chips

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3829-3840 ◽  
Author(s):  
Amélie St-Georges-Robillard ◽  
Mathieu Masse ◽  
Maxime Cahuzac ◽  
Mathias Strupler ◽  
Bishnubrata Patra ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Cell Populations ◽  
Wide Field ◽  
Microfluidic Chips ◽  
Tumor Spheroids ◽  
On Chip

Wide-field hyperspectral imaging to follow cell populations in co-culture tumor spheroids on chip.

scholarly journals Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 576 ◽  
Author(s):  
Edgar Jiménez-Díaz ◽  
Mariel Cano-Jorge ◽  
Diego Zamarrón-Hernández ◽  
Lucia Cabriales ◽  
Francisco Páez-Larios ◽  
...  
Keyword(s):  
Soft Lithography ◽  
Low Cost ◽  
Cost Effective ◽  
Microfluidic Chips ◽  
Single Chip ◽  
Lab On Chip ◽  
Molding Method ◽  
On Chip ◽  
Selective Integration ◽  
Important Progress

Microfluidics has become a very promising technology in recent years, due to its great potential to revolutionize life-science solutions. Generic microfabrication processes have been progressively made available to academic laboratories thanks to cost-effective soft-lithography techniques and enabled important progress in applications like lab-on-chip platforms using rapid- prototyping. However, micron-sized features are required in most designs, especially in biomimetic cell culture platforms, imposing elevated costs of production associated with lithography and limiting the use of such devices. In most cases, however, only a small portion of the structures require high-resolution and cost may be decreased. In this work, we present a replica-molding method separating the fabrication steps of low (macro) and high (micro) resolutions and then merging the two scales in a single chip. The method consists of fabricating the largest possible area in inexpensive macromolds using simple techniques such as plastics micromilling, laser microfabrication, or even by shrinking printed polystyrene sheets. The microfeatures were made on a separated mold or onto existing macromolds using photolithography or 2-photon lithography. By limiting the expensive area to the essential, the time and cost of fabrication can be reduced. Polydimethylsiloxane (PDMS) microfluidic chips were successfully fabricated from the constructed molds and tested to validate our micro–macro method.

Inkjet 3D printed modular microfluidic chips for on-chip gel electrophoresis

2019 ◽  
Vol 29 (5) ◽  
pp. 057001 ◽  
Author(s):  
Rafał Walczak ◽  
Krzysztof Adamski ◽  
Wojciech Kubicki
Keyword(s):  
Gel Electrophoresis ◽  
Microfluidic Chips ◽  
3D Printed ◽  
On Chip

scholarly journals Cross-Flow Filtration of Escherichia coli at a Nanofluidic Gap for Fast Immobilization and Antibiotic Susceptibility Testing

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 691 ◽  
Author(s):  
Jan F. Busche ◽  
Svenja Möller ◽  
Matthias Stehr ◽  
Andreas Dietzel
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Cross Flow ◽  
Test Time ◽  
Microfluidic Chips ◽  
Cross Flow Filtration ◽  
Antibiotic Susceptibility Test ◽  
Flow Filtration ◽  
On Chip

Infections with antimicrobial-resistant (AMR) bacteria are globally on the rise. In the future, multi-resistant infections will become one of the major problems in global health care. In order to enable reserve antibiotics to retain their effect as long as possible, broad-spectrum antibiotics must be used sparingly. This can be achieved by a rapid microfluidic phenotypic antibiotic susceptibility test, which provides the information needed for a targeted antibiotic therapy in less time than conventional tests. Such microfluidic tests must cope with a low bacteria concentration. On-chip filtering of the samples to accumulate bacteria can shorten the test time. By means of fluorescence microscopy, we examined a novel nanogap filtration principle to hold back Escherichia coli and to perform cultivation experiments with and without antibiotics present. Microfluidic chips based on the nanogap flow principle showed to be useful for the concentration and cultivation of E. coli. With a concentration of 106 cells/mL, a specific growth rate of 0.013 min−1 and a doubling time of 53 min were achieved. In the presence of an antibiotic, no growth was observed. The results prove that this principle can, in future, be used in fast and marker-free antimicrobial susceptibility testing (AST).

Inhibition of on-chip PCR using PDMS–glass hybrid microfluidic chips

2012 ◽  
Vol 13 (3) ◽  
pp. 383-398 ◽  
Author(s):  
H. John Crabtree ◽  
Jana Lauzon ◽  
Yuen C. Morrissey ◽  
Brian J. Taylor ◽  
Tina Liang ◽  
...  
Keyword(s):  
Microfluidic Chips ◽  
On Chip

scholarly journals Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

Biosensors ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 66
Author(s):  
Águeda Molinero-Fernández ◽  
María Moreno-Guzmán ◽  
Miguel Ángel López ◽  
Alberto Escarpa
Keyword(s):  
Limit Of Detection ◽  
Magnetic Bead ◽  
Clinical Analysis ◽  
Good Precision ◽  
Microfluidic Chips ◽  
Reliable Determination ◽  
Clinical Sepsis ◽  
Sepsis Diagnosis ◽  
On Chip ◽  
Electrochemical Immunoassays

Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

Development of Microfluidic Chips to Study the Effects of Shear Stress on Cell Functions

2010 ◽  
Author(s):  
Jianbin Wang ◽  
Jinseok Heo ◽  
Susan Z. Hua
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Cellular Systems ◽  
Shear Stresses ◽  
Microfluidic Chips ◽  
Fluid Shear ◽  
Cell Functions ◽  
Culture Cells ◽  
Wide Range ◽  
On Chip

Fluid shear stress has profound effect on many cell functions, including proliferation, migration, transport, and gene expression. Cellular systems such as endothelial cells in heart artery and epithelial cells in kidney tubule are constantly subject to fluid flow. We have developed a series of microfluidic chips that generate a wide range and modes of shear stresses within a perfusion chamber, enabling us to culture cells on chip and examine the effects of shear stress on cell growth and cell functions.

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