scholarly journals On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

AIP Advances ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 095213 ◽  
Author(s):  
Hiroaki Takehara ◽  
Osawa Kazutaka ◽  
Makito Haruta ◽  
Toshihiko Noda ◽  
Kiyotaka Sasagawa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Analysis ◽  
Microfluidic Chips ◽  
On Chip ◽  
Analysis Platform

scholarly journals Massively parallel quantification of phenotypic heterogeneity in single cell drug responses

2020 ◽  
Author(s):  
Benjamin B. Yellen ◽  
Jon S. Zawistowski ◽  
Eric A. Czech ◽  
Caleb I. Sanford ◽  
Elliott D. SoRelle ◽  
...  
Keyword(s):  
Machine Learning ◽  
Acute Myeloid Leukemia ◽  
Single Cell ◽  
Myeloid Leukemia ◽  
Single Cell Analysis ◽  
Phenotypic Heterogeneity ◽  
Massively Parallel ◽  
Cell Analysis ◽  
On Chip ◽  
Acute Myeloid

AbstractSingle cell analysis tools have made significant advances in characterizing genomic heterogeneity, however tools for measuring phenotypic heterogeneity have lagged due to the increased difficulty of handling live biology. Here, we report a single cell phenotyping tool capable of measuring image-based clonal properties at scales approaching 100,000 clones per experiment. These advances are achieved by exploiting a novel flow regime in ladder microfluidic networks that, under appropriate conditions, yield a mathematically perfect cell trap. Machine learning and computer vision tools are used to control the imaging hardware and analyze the cellular phenotypic parameters within these images. Using this platform, we quantified the responses of tens of thousands of single cell-derived acute myeloid leukemia (AML) clones to targeted therapy, identifying rare resistance and morphological phenotypes at frequencies down to 0.05%. This approach can be extended to higher-level cellular architectures such as cell pairs and organoids and on-chip live-cell fluorescence assays.

Dynamic microfluidic nanocalorimetry system for measuring Caenorhabditis elegans metabolic heat

Lab on a Chip ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 1641-1651 ◽  
Author(s):  
Roger Krenger ◽  
Thomas Lehnert ◽  
Martin A. M. Gijs
Keyword(s):  
Caenorhabditis Elegans ◽  
Microfluidic Chips ◽  
C Elegans ◽  
Metabolic Heat ◽  
On Chip

A nanocalorimetry system with integrated microfluidic chips for on-chip measurements of metabolic heat signals of C. elegans.

(Invited) On-Chip Characterization of Microcapsules Using a Capacitive Sensor for Microencapsulation and Single-Cell Analysis Applications

2021 ◽  
Vol MA2021-01 (60) ◽  
pp. 1603-1603
Author(s):  
Sajjad Janfaza ◽  
Seyedehhamideh Razavi ◽  
Arash Dalili ◽  
Mina Hoorfar
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Capacitive Sensor ◽  
Cell Analysis ◽  
On Chip

scholarly journals Wide Field-of-View On-Chip Talbot Fluorescence Microscopy for Longitudinal Cell Culture Monitoring from within the Incubator

2013 ◽  
Vol 85 (4) ◽  
pp. 2356-2360 ◽  
Author(s):  
Chao Han ◽  
Shuo Pang ◽  
Danielle V. Bower ◽  
Patrick Yiu ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Fluorescence Microscopy ◽  
Field Of View ◽  
Wide Field ◽  
Wide Field Of View ◽  
On Chip ◽  
Longitudinal Cell ◽  
Culture Monitoring

Integrated on-chip lens applied to microfluidic chips

2012 ◽  
Author(s):  
Yingying Zhao ◽  
Qin Li ◽  
Xiao-Ming Hu ◽  
Dong-Fang Yang
Keyword(s):  
Microfluidic Chips ◽  
On Chip

Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips

2008 ◽  
Vol 29 (24) ◽  
pp. 5055-5060 ◽  
Author(s):  
Linfen Yu ◽  
Huaiqing Huang ◽  
Xiuling Dong ◽  
Dapeng Wu ◽  
Jinhua Qin ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Microfluidic Chips

Surface Microfabrication of Conventional Glass Using Femtosecond Laser for Microfluidic Applications

2017 ◽  
Vol 11 (6) ◽  
pp. 878-882 ◽  
Author(s):  
Takuma Niioka ◽  
◽  
Yasutaka Hanada
Keyword(s):  
High Resolution ◽  
Microfluidic Chip ◽  
High Speed ◽  
Single Cell Analysis ◽  
Glass Slide ◽  
Cell Analysis ◽  
Microfluidic Chips ◽  
Direct Writing ◽  
Laser Direct Writing ◽  
Fs Laser

Recently, a lot of attention has been paid to a single-cell analysis using microfluidic chips, since each cell is known to have several different characteristics. The microfluidic chip manipulates cells and performs high-speed and high-resolution analysis. In the meanwhile, femtosecond (fs) laser has become a versatile tool for the fabrication of microfluidic chips because the laser can modify internal volume solely at the focal area, resulting in three-dimensional (3D) microfabrication of glass materials. However, little research on surface microfabrication of materials using an fs laser has been conducted. Therefore, in this study, we demonstrate the surface microfabrication of a conventional glass slide using fs laser direct-writing for microfluidic applications. The fs laser modification, with successive wet etching using a diluted hydrofluoric (HF) acid solution, followed by annealing, results in rapid prototyping of microfluidics on a conventional glass slide for fluorescent microscopic cell analysis. Fundamental characteristics of the laser-irradiated regions in each experimental procedure were investigated. In addition, we developed a novel technique combining the fs laser direct-writing and the HF etching for high-speed and high-resolution microfabrication of the glass. After establishing the fs laser surface microfabrication technique, a 3D microfluidic chip was made by bonding the fabricated glass microfluidic chip with a polydimethylsiloxane (PDMS) polymer substrate for clear fluorescent microscopic observation in the microfluidics.

Vision-Based Real-Time Microflow-Rate Control System for Cell Analysis

2016 ◽  
Vol 28 (6) ◽  
pp. 854-861 ◽  
Author(s):  
Tadayoshi Aoyama ◽  
◽  
Amalka De Zoysa ◽  
Qingyi Gu ◽  
Takeshi Takaki ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Control System ◽  
Real Time ◽  
Flow Rate ◽  
Rate Control ◽  
Microfluidic Devices ◽  
Cell Analysis ◽  
Time Flow ◽  
On Chip ◽  
Flow Rate Control

[abstFig src='/00280006/09.jpg' width='300' text='Snapshots of particle sorting experiment using our system' ] On-chip cell analysis is an important issue for microtechnology research, and microfluidic devices are frequently used in on-chip cell analysis systems. One approach to controlling the fluid flow in microfluidic devices for cell analysis is to use a suitable pumps. However, it is difficult to control the actual flow-rate in a microfluidic device because of the difficulty in placing flow-rate sensors in the device. In this study, we developed a real-time flow-rate control system that uses syringe pumps and high-speed vision to measure the actual fluid flow in microfluidic devices. The developed flow-rate control system was verified through experiments on microparticle velocity control and microparticle sorting.

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