Photophysics of water-soluble Cu(II)-porphyrin: groove-bounding to polynucleotide as compared to dissolving in neat buffer solution

1995 ◽  
Author(s):  
Vladimir A. Galievsky ◽  
Vladimir S. Chirvony ◽  
Laurent Chinsky ◽  
Pierre-Yves Turpin
Keyword(s):  
Water Soluble ◽  
Buffer Solution

THE METABOLISM OF VALERATE-2-C14 BY UREDOSPORES OF WHEAT STEM RUST

1963 ◽  
Vol 41 (1) ◽  
pp. 1-7 ◽  
Author(s):  
H. Reisener ◽  
A. J. Finlayson ◽  
W. B. McConnell
Keyword(s):  
Carbon Dioxide ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Tricarboxylic Acid Cycle ◽  
High Specific Activity ◽  
Water Soluble ◽  
Buffer Solution ◽  
Wheat Stem Rust ◽  
Carbon 14 ◽  
Water Soluble Extract

When uredospores of Puccinia graminis var. tritici race 15B were shaken in a medium containing M/30 phosphate buffer, pH 6.2, and valerate-2-C14, about 88% of the radioactivity was removed from the buffer solution in a period of 3 hours. About 40% of the carbon-14 taken from the buffer was found in a water-soluble extract of the spores and about 15% was respired as carbon dioxide. The result is compared with an earlier report that carbon 1 of valerate is more extensively released as carbon dioxide and less extensively incorporated into spore components. Glutamic acid, glutamine, γ-aminobutyric acid, and alanine of high specific activity were isolated. It was estimated from partial degradation that more than one-half of the carbon-14 of glutamic acid occurred in position 4 and that carbon 5 was very weakly labelled. Citric acid was also of high specific activity and was labelled predominantly in the internal carbons.It is concluded that respiring rust spores utilize externally supplied valerate by β-oxidation, which releases carbons 1 and 2 in a form which is metabolized as acetate by the tricarboxylic acid cycle.

scholarly journals Synthesis of a water-soluble 2,2′-biphen[4]arene and its efficient complexation and sensitive fluorescence enhancement towards palmatine and berberine

2018 ◽  
Vol 14 ◽  
pp. 2236-2241 ◽  
Author(s):  
Xiayang Huang ◽  
Xinghua Zhang ◽  
Tianxin Qian ◽  
Junwei Ma ◽  
Lei Cui ◽  
...  
Keyword(s):  
Association Constant ◽  
Fluorescence Enhancement ◽  
Phosphate Buffer Solution ◽  
Water Soluble ◽  
Binding Affinities ◽  
Buffer Solution ◽  
Solution Ph ◽  
H Nmr ◽  
Naked Eye ◽  
Nmr Signals

A water-soluble 2,2′-biphen[4]arene (2,2’-CBP4) containing eight carboxylato moieties was synthesized and characterized. Its complexation behavior towards two alkaloids, palmatine (P) and berberine (B), was investigated by means of fluorescence and 1H NMR spectroscopy in aqueous phosphate buffer solution (pH 7.4). In the presence of 2,2’-CBP4, 1H NMR signals of P and B displayed very large upfield shifts, indicating the formation of inclusion complexes with strong binding affinities. Fluorescence titration experiments showed that P and B exhibited dramatic fluorescence enhancement of more than 600 times upon complexation with 2,2’-CBP4. Particularly, the fluorescence intensity is strong enough to be readily distinguished by the naked eye. Although the two guests have similar structures, the association constant of B with 2,2’-CBP4 (K a = (2.29 ± 0.27) × 106 M−1) is 3.9 times larger than that of P (K a = (5.87 ± 0.24) × 105 M−1).

A water-soluble sulfonate-BODIPY based fluorescent probe for selective detection of HOCl/OCl−in aqueous media

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 2986-2989 ◽  
Author(s):  
Jiyoung Kim ◽  
Youngmi Kim
Keyword(s):  
Electron Transfer ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Photoinduced Electron Transfer ◽  
Aqueous Media ◽  
Water Soluble ◽  
Selective Detection ◽  
Buffer Solution ◽  
Aqueous Buffer ◽  
Aqueous Buffer Solution

A water-soluble sulfonate-BODIPY dye1was developed for the selective detection of HOCl/OCl−in aqueous buffer solution. The probe, which displays extremely weak fluorescence owing to efficient photoinduced electron transfer (PeT) from the pendant catechol donor, responds to HOCl/OCl−through a dramatic enhancement of its fluorescence intensity.

Determination of Trace Iron in Chinese Herbal Medicine Using CdS Quantum Dots as Fluorescence Probes

2012 ◽  
Vol 455-456 ◽  
pp. 1195-1199
Author(s):  
Yun Long Zeng ◽  
Xia Fei Li ◽  
Chun Ran Tang ◽  
Hao Wen Huang ◽  
Ya Jing Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Probe ◽  
Copper Ion ◽  
Water Soluble ◽  
Fluorescence Probes ◽  
Cds Quantum Dots ◽  
Buffer Solution ◽  
Green Protocol ◽  
Quenching Method

A simple A green protocol for synthesis of high quality, water-soluble and biocompatible CdS QDs have been developed in aqueous solution with amylum as stabilizing agent. The QDs were characterized by AMF and spectral methods. A fluorescence quenching method was developed for determination of iron (ΙΙΙ) with water soluble CdS quantum dots (QDs) as fluorescence probe. In pH 8.5 boric acid–borax buffer solution, the QDs show a high selective to ferric ion. The common cations, such as Ca2+, Mg2+, Zn2+, Fe2+ et al, have no effect on interference but copper ion. The fluorescence intensity of the QDs decreased linearly with the iron (QDs ) concentration in the range of 200 μM to 60 nM with a detection limit of 20 nM. It has been applied to determinate of iron in Angelica, Astragalus, Shouwu, ligusticum wallichii and radix rehmanniae recen.

scholarly journals INTERPHASE DISTRIBUTION OF SOME AMINO ACIDS IN EXTRACTION SYSTEMS BASED ON N-VINYLFORMAMIDE COPOLYMERS

2019 ◽  
Vol 62 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Nadezhda Ya. Mokshina ◽  
Oksana A. Pakhomova ◽  
Gennadiy V. Shatalov ◽  
Irina I. Kosinova
Keyword(s):  
Amino Acids ◽  
Radical Copolymerization ◽  
Distribution Coefficients ◽  
Water Soluble ◽  
Separate Determination ◽  
Buffer Solution ◽  
Salting Out ◽  
Interphase Distribution ◽  
Degree Of Extraction

The extraction characteristics of amino acids and mono-amides of acids were studied using copolymers of N-vinylformamide with 1-vinyl- and 1-methacryloyl-3,5-dimethylpyrazole as extractants. Synthesis of water-soluble copolymers was carried out by radical copolymerization in dioxane with thermoinitiation with dinitro-azobisisomoic acid. The distribution coefficients and the degree of extraction of analytes for a single extraction in the presence of a salting out agent are calculated. To determine the representatives of amino acids, the method of capillary electrophoresis was used. The most effective extraction systems for the extraction of amino acids and monoamides of acids are proposed: the intrinsic viscosity of polymers, the concentration of polymer and analyte solution, the ratio of phase volumes, the ratio of the mole fractions of N-vinylformamide to 1-vinyl and 1-methacryloyl-3,5-dimethylpyrazole. The results of the most effective interfacial distribution of lysine between a water-salt solution and an extractant are presented, which is a copolymer of N-vinylformamide with 1-methacryloyl-3,5-dimethylpyrazole. The concentration of the copolymer and the ratio of the volumes of equilibrium phases at which the maximum degree of lysine extraction is reached are established. A copolymer of N-vinylformamide with 1-vinyl-3,5-dimethylpyrazole is used for simultaneous extraction of cystine, asparagine and glutamine. The established extraction characteristics allowed a successful separate determination of the amino acid cystine and monoamides (asparagine and glutamine) in a joint presence in an aqueous solution. When choosing the conditions for separate electrophoretic determination of amino acids, the optimal composition of the buffer solution, the type and concentration of the micelle was found. The dependence of the structure of the analytes on their interphase distribution was established, and the complexing power of the copolymers of N-vinylformamide with 1-vinyl- and 1-methacryloyl-3,5-dimethylpyrazole was revealed with respect to the representatives of amino acids. The extraction systems used on the basis of N-vinylformamide copolymers are distinguished by ecological and economic expediency, good metrological indices.

scholarly journals Tunneling in the Hydrogen-Transfer Reaction from a Vitamin E Analog to an Inclusion Complex of 2,2-Diphenyl-1-picrylhydrazyl Radical with β-Cyclodextrin in an Aqueous Buffer Solution at Ambient Temperature

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1966
Author(s):  
Ikuo Nakanishi ◽  
Yoshimi Shoji ◽  
Kei Ohkubo ◽  
Shunichi Fukuzumi
Keyword(s):  
Vitamin E ◽  
Phosphate Buffer ◽  
Hydrogen Transfer ◽  
Transfer Reaction ◽  
Phosphate Buffer Solution ◽  
Water Soluble ◽  
Quantum Mechanical ◽  
Buffer Solution ◽  
Quantum Mechanical Tunneling ◽  
Hydrogen Transfer Reaction

Recently, increasing attention has been paid to quantum mechanical behavior in biology. In this study, we investigated the involvement of quantum mechanical tunneling in the hydrogen-transfer reaction from Trolox, a water-soluble analog of vitamin E (α-tocopherol), to 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) in a phosphate buffer solution (0.05 M, pH 7.0). DPPH• was used as a reactivity model of reactive oxygen species and solubilized in water using β-cyclodextrin (β-CD). The second-order rate constants, kH and kD, in 0.05 M phosphate buffer solutions prepared with H2O (pH 7.0) and D2O (pD 7.0), respectively, were determined for the reaction between Trolox and DPPH•, using a stopped-flow technique at various temperatures (283–303 K). Large kinetic isotope effects (KIE, kH/kD) were observed for the hydrogen-transfer reaction from Trolox to the β-CD-solubilized DPPH• in the whole temperature range. The isotopic ratio of the Arrhenius prefactor (AH/AD = 0.003), as well as the isotopic difference in the activation energies (19 kJ mol−1), indicated that quantum mechanical tunneling plays a role in the reaction.

scholarly journals The influence of soil steaming on some properties of the soil and on the growth and heading of winter glasshouse lettuce. I. Changes in chemical and physical properties.

1969 ◽  
Vol 17 (2) ◽  
pp. 143-152 ◽  
Author(s):  
G. Jager ◽  
J. Van der Boon ◽  
G.J. Rauw
Keyword(s):  
Physical Properties ◽  
Negative Correlation ◽  
Water Soluble ◽  
Buffer Solution ◽  
Mineral N ◽  
P Content ◽  
Mn Content ◽  
Mg Content

Soil steaming caused an increase of 33 ppm mineral N. There was no significant effect on water-soluble P2O5. The influence of steaming on buffer-soluble Mg content varied with the treatment but there seemed to be a negative correlation with the P content. Steaming enhanced K solubility and increased buffer-soluble Mn content. Fe and Al soluble in buffer solution were slightly increased. 44 references. (Abstract retrieved from CAB Abstracts by CABI’s permission)

One-pot synthesis of cellulose-based nonwoven web incorporated with chitosan for hemostat applications

2020 ◽  
Vol 35 (2) ◽  
pp. 92-101
Author(s):  
T Hemamalini ◽  
N Vikash ◽  
P Brindha ◽  
M Abinaya ◽  
VR Giri Dev
Keyword(s):  
Blood Clotting ◽  
Phosphate Buffer Solution ◽  
Wood Pulp ◽  
Water Soluble ◽  
Pulp Fiber ◽  
Buffer Solution ◽  
One Pot ◽  
Chitosan Concentration ◽  
Severe Hemorrhage ◽  
Solution Uptake

Water-soluble chitosan and wood pulp fiber–based nonwovens were produced using wet-laying technology, and their properties were investigated for the potential application for severe hemorrhage. The pores of the wood pulp nonwovens were completely covered as the concentration of chitosan was increased. A phosphate buffer solution uptake of 997% was attained in the nonwoven loaded with chitosan concentration of 1.5 w/v%. The deposition of blood cells was found to increase with an increase in the water-soluble chitosan concentration. The blood-clotting time was found to be 170 s, making the developed nonwoven to promote blood-clotting mechanism by creating mechanical barrier to reduce the blood loss.

scholarly journals FORMULATION AND EVALUATION OF LUMEFANTRINE CAPSULE PREPARED BY USING LIQUISOLID TECHNIQUE

2018 ◽  
Vol 10 (2) ◽  
pp. 43 ◽  
Author(s):  
Amreen Khan ◽  
Shikha Agrawal
Keyword(s):  
Uv Spectroscopy ◽  
Research Work ◽  
Amorphous State ◽  
Tween 80 ◽  
Water Soluble ◽  
In Vitro Dissolution ◽  
Buffer Solution ◽  
Capsule Formulation ◽  
Promising Alternative ◽  
Drug Sample

Objective: The objective of present research work was to formulate and evaluate of Lumefantrine capsule by using novel liquisolid technique to give increased dissolution rate of poorly water-soluble drug Lumefantrine.Methods: Formulation of lumefantrine capsule using drug solution and suspension method was used. Different liquisolid formulations were prepared using a mathematical model for calculating required quantities of powder and liquid ingredients to produce an acceptably flowable and compressible admixture. Liquisolid capsule formulation F-1 to F-9 were prepared by using different type and different concentration of non-volatile solvent like PEG-400, Tween 80, propylene glycol and avicels, aerosil as carrier and coating material respectively.Results: The liquisolid formulation were within the acceptable limits and drug release rates of all prepared liquisolid were distinctly higher as compared to pure drug. Lumefantrine shows maximum solubility in tween 80 as a non-volatile solvent. All the preformulation parameters were evaluated such as organoleptic characterization of the drug sample, melting point, pH, identification of drug samples by using UV spectroscopy and FTIR analytical method, preparation of calibration curves, solubility studies of drug sample like qualitative, quantitative and pH-dependent solubility of the drug in a buffer solution of different pH. They were further processed for solid-state characterization such as, DSC and SEM and the results confirmed the transformation of native crystalline nature of drug to an amorphous state. FTIR analysis also confirmed no drug-excipient interaction. Liquisolid formulations showed improved in vitro dissolution behaviour of lumefantrine over that of pure drug.Conclusion: From this study, it was concluded that liquisolid method is a promising alternative for improvement of dissolution property of water-insoluble drugs.

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