Center for Fluorescence Spectroscopy: advanced studies of fluorescence dynamics, lifetime imaging, clinical sensing, two-photon excitation, and light quenching

1994 ◽  
Author(s):  
Joseph R. Lakowicz ◽  
Henryk M. Malak ◽  
Ignacy Gryczynski ◽  
Henryk Szmacinski ◽  
Jozef Kusba ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Light Quenching ◽  
Two Photon Excitation

Recent Developments in Fluorescence Spectroscopy: Light Quenching and Multi-Photon Excitation with Tir, Two-Color Two-Photon Excitation and Long Lifetime Probes

1997 ◽  
Vol 3 (S2) ◽  
pp. 793-794
Author(s):  
Joseph R. Lakowicz
Keyword(s):  
Fluorescence Spectroscopy ◽  
Evanescent Wave ◽  
Cell Biology ◽  
Incident Angle ◽  
Selective Excitation ◽  
Two Photon ◽  
Fluorescence Measurements ◽  
Photon Excitation ◽  
Light Quenching ◽  
Two Photon Excitation

Fluorescence spectroscopy is presently enjoying explosive growth due to new experimental capabilities. In this talk we summarize some recent advances which can have applications in cell biology and imaging.We show that evanescent illumination at one wavelength and incident angle, coupled with light quenching at a second wavelength and incident angle (Figure 1), can be used for selective excitation of fluorophores located up to 5000 Å into the aqueous phase. The use of combined evanescent wave excitation and evanescent wave quenching could provide selective excitation of fluorophores in the cytoplasmic region of cells. We also reported the first experimental observation of two-photon excitation using the evanescent wave resulting from total internal reflection. Two-photon excitation of the calcium probe Indo-1 was demonstrated by the quadratic dependence on intensity (Figure 2) as well as by the anisotropy and time-resolved fluorescence measurements.Two-photon excitation is routinely performed using a single wavelength. We recently observed two-photon excitation of organic fluorophores with two different wavelengths, a phenomenon we refer to as two-color two-photon excitation.

Fluorescence spectroscopy of biological tissue: single- and two-photon excitation

2004 ◽  
Author(s):  
Yicong Wu ◽  
Peng Xi ◽  
Weikun Ge ◽  
Powing Yuen ◽  
Jianan Y. Qu
Keyword(s):  
Fluorescence Spectroscopy ◽  
Biological Tissue ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Applications of Two-Photon Excitation Fluorescence Lifetime Imaging in Tumor Diagnosis

2018 ◽  
Vol 45 (2) ◽  
pp. 0207010
Author(s):  
李慧 Li Hui ◽  
夏先园 Xia Xianyuan ◽  
陈廷爱 Chen Tingai ◽  
余佳 Yu Jia ◽  
李曦 Li Xi ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Tumor Diagnosis ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Two Photon Excitation
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