Picosecond laser studies of the solvent-dependent nonradiative pathways in near-IR fluorescent dyes: implications on their use in ultrasensitive analysis

1994 ◽  
Author(s):  
Steven A. Soper ◽  
Quincy L. Mattingly ◽  
Benjamin L. Legendre, Jr.
Keyword(s):  
Fluorescent Dyes ◽  
Picosecond Laser ◽  
Near Ir

Heavy-atom modified near-IR fluorescent dyes for DNA sequencing applications: synthesis and photophysical characterization

1997 ◽  
Author(s):  
James H. Flanagan, Jr. ◽  
Sarah E. Romero ◽  
Benjamin L. Legendre, Jr. ◽  
Robert P. Hammer ◽  
Steven A. Soper
Keyword(s):  
Dna Sequencing ◽  
Fluorescent Dyes ◽  
Heavy Atom ◽  
Near Ir

Chemistry at Boron: Synthesis and Properties of Red to Near-IR Fluorescent Dyes Based on Boron-Substituted Diisoindolomethene Frameworks

2011 ◽  
Vol 76 (11) ◽  
pp. 4489-4505 ◽  
Author(s):  
Gilles Ulrich ◽  
Sébastien Goeb ◽  
Antoinette De Nicola ◽  
Pascal Retailleau ◽  
Raymond Ziessel
Keyword(s):  
Fluorescent Dyes ◽  
Near Ir

Extending the Conjugation of Pechmann Lactone Thienyl Derivatives: A New Class of Small Molecules for Organic Electronics Application

Synthesis ◽  
2017 ◽  
Vol 50 (06) ◽  
pp. 1284-1292 ◽  
Author(s):  
Massimo Calamante ◽  
Gianna Reginato ◽  
Alessio Dessì ◽  
Matteo Bartolini ◽  
Lorenzo Zani ◽  
...  
Keyword(s):  
Small Molecules ◽  
Organic Electronics ◽  
Fluorescent Dyes ◽  
Photophysical Properties ◽  
Emission Spectra ◽  
Coupling Reaction ◽  
Spectroscopic Characterization ◽  
Synthetic Approach ◽  
New Class ◽  
Near Ir

The preparation and spectroscopic characterization of some symmetrical small molecules containing the disubstituted (E)-3,3′-bifuranylidene-2,2′-dione chromophore (the so-called Pechmann lactone) and featuring an extended conjugation are reported. The synthetic approach of such compounds is based on the Stille–Migita coupling reaction, which is carried out in very mild conditions, suitable to be applied with the very sensitive Pechmann core. The absorption and emission spectra of the new molecules obtained have been recorded in solution and clearly show how their photophysical properties can be modulated by a proper choice of the (hetero)aromatic terminal groups. The compounds prepared in this study (or close analogues thereof) have interesting optical properties and could find application as semiconductors in organic electronics or as near-IR fluorescent dyes.

Single-lane single-fluor sequencing using dideoxy-labeled, heavy-atom-modified near-IR fluorescent dyes

1995 ◽  
Author(s):  
Daryl C. Williams ◽  
James H. Flanagan, Jr. ◽  
Benjamin L. Legendre, Jr. ◽  
Robert P. Hammer ◽  
Steven A. Soper
Keyword(s):  
Fluorescent Dyes ◽  
Heavy Atom ◽  
Near Ir

Surface Brightness Scale of Galactic Cepheids

1999 ◽  
Vol 190 ◽  
pp. 561-562
Author(s):  
G. P. Di Benedetto
Keyword(s):  
Surface Brightness ◽  
Variable Stars ◽  
Giant Stars ◽  
Near Ir

An accurate calibration of the surface brightness scaleSVas a function of the near-IR color (V–K) has been recently measured for non-variable Galactic dwarf and giant stars. It can be shown that this correlation can be applied to theSVscale of Galactic Cepheid variable stars, which are of major cosmological interest.

Conformation of Ribosomes from Escherichia Coli

1974 ◽  
Vol 32 ◽  
pp. 210-211
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Binding Sites ◽  
Ribosomal Proteins ◽  
Fluorescent Dyes ◽  
Protein Biosynthesis ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Dimensional Structure ◽  
Complete Understanding ◽  
And Function

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.

Analysis of 3-d molecular distribution with the digital imaging microscope

1988 ◽  
Vol 46 ◽  
pp. 40-41
Author(s):  
W.A. Carrington ◽  
F.S. Fay ◽  
K.E. Fogarty ◽  
L. Lifshitz
Keyword(s):  
Image Restoration ◽  
Digital Imaging ◽  
Fluorescent Dyes ◽  
Optical Axis ◽  
Computer Hardware ◽  
Computer Algorithms ◽  
Low Contrast ◽  
Specific Proteins ◽  
Effective Use ◽  
Single Living Cells

Advances in digital imaging microscopy and in the synthesis of fluorescent dyes allow the determination of 3D distribution of specific proteins, ions, GNA or DNA in single living cells. Effective use of this technology requires a combination of optical and computer hardware and software for image restoration, feature extraction and computer graphics.The digital imaging microscope consists of a conventional epifluorescence microscope with computer controlled focus, excitation and emission wavelength and duration of excitation. Images are recorded with a cooled (-80°C) CCD. 3D images are obtained as a series of optical sections at .25 - .5 μm intervals.A conventional microscope has substantial blurring along its optical axis. Out of focus contributions to a single optical section cause low contrast and flare; details are poorly resolved along the optical axis. We have developed new computer algorithms for reversing these distortions. These image restoration techniques and scanning confocal microscopes yield significantly better images; the results from the two are comparable.

High-resolution measurement of birefringent fine structure in living cells using a new polarized light microscope

1995 ◽  
Vol 53 ◽  
pp. 54-55
Author(s):  
Rudolf Oldenbourg
Keyword(s):  
Light Microscope ◽  
Fluorescent Dyes ◽  
Polarized Light ◽  
Processing System ◽  
Video Camera ◽  
Molecular Organization ◽  
Computer Assisted ◽  
Image Recording ◽  
Birefringent Crystal ◽  
Technological Advances

The recent renaissance of the light microsope is fueled in part by technological advances in components on the periphery of the microscope, such as the laser as illumination source, electronic image recording (video), computer assisted image analysis and the biochemistry of fluorescent dyes for labeling specimens. After great progress in these peripheral parts, it seems timely to examine the optics itself and ask how progress in the periphery facilitates the use of new optical components and of new optical designs inside the microscope. Some results of this fruitful reflection are presented in this symposium.We have considered the polarized light microscope, and developed a design that replaces the traditional compensator, typically a birefringent crystal plate, with a precision universal compensator made of two liquid crystal variable retarders. A video camera and digital image processing system provide fast measurements of specimen anisotropy (retardance magnitude and azimuth) at ALL POINTS of the image forming the field of view. The images document fine structural and molecular organization within a thin optical section of the specimen.

En face dual fluorescence staining of fatty streaks allows observation of initiation and progression of atherosclerotic lesions

1995 ◽  
Vol 53 ◽  
pp. 864-865
Author(s):  
Anne M. Klinkner ◽  
Crystal R. Waites ◽  
Peter J. Bugelski ◽  
William D. Kerns
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Dimethyl Formamide ◽  
High Cholesterol Diet ◽  
Methods Development ◽  
Atherosclerotic Lesions ◽  
En Face ◽  
Biochemical Evaluation ◽  
Stock Solutions ◽  
Dual Staining

A primary effort in the understanding of the progression of atherosclerotic disease has been methods development for visualization of the atherosclerotic plaque. We introduce a new method for the qualitative analysis of lipids in atherosclerotic fatty streaks which also retains those lipids for biochemical evaluation. An original aspect of the process is the ability to view an entire fatty streak en face, selectively stained for specific lipid classes within the lesion.New Zealand white rabbits were fed a high cholesterol diet(0.15%-0.3% for 14 wks). The aorta was removed and fixed in Carson's phosphate buffered formaldehyde followed by dual staining in the fluorescent dyes Nile red and filipin. Stock solutions of nile red(0.5mg/ml acetone) and filipin(2.5mg/ml dimethyl formamide) were prepared and kept at -20°C; all subsequent steps were at RT. 0.5cm × 1.0cm pieces of aorta were trimmed and adventitia removed. The pieces were then washed 3×15 min in PBS w/o CaMg, soaked in Nile red(NR)/filipin(Fl) stain(100(il NR stock + 200μl Fl stock in 10 ml PBS for 30 min, washed in PBS 3×30 min, rinsed with distilled water, mounted(Crystal Mount, Biomedia) and coverslipped and viewed by fluorescence microscopy.

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