Improvements in semiautomated serial section reconstruction and visualization of neural tissue from transmission electron microscope images

1994 ◽  
Author(s):  
Kevin N. Montgomery ◽  
Muriel D. Ross
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Serial Section ◽  
Neural Tissue ◽  
Transmission Electron ◽  
Microscope Images ◽  
Serial Section Reconstruction

scholarly journals Simulation of Transmission Electron Microscope Images of Dislocations Pinned by Obstacles

2014 ◽  
Vol 55 (3) ◽  
pp. 413-417
Author(s):  
Yuhki Satoh ◽  
Takahiro Hatano ◽  
Nobuyasu Nita ◽  
Kimihiro Nogiwa ◽  
Hideki Matsui
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Transmission Electron ◽  
Microscope Images

scholarly journals Long-term incubation of lake water enables genomic sampling of consortia involving Planctomycetes and Candidate Phyla Radiation bacteria

2021 ◽  
Author(s):  
Alexander L Jaffe ◽  
Maxime Fuster ◽  
Marie C Schoelmerich ◽  
Lin-Xing Chen ◽  
Jonathan Colombet ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Culture Conditions ◽  
Interaction Networks ◽  
Biogeochemical Processes ◽  
Transmission Electron ◽  
Microscope Images ◽  
Collective Interactions ◽  
Candidate Phyla Radiation

Microbial communities in lakes can profoundly impact biogeochemical processes through their individual activities and collective interactions. However, the complexity of these communities poses challenges, particularly for studying rare members. Laboratory enrichments can select for subsystems of interacting organisms and enable genome recovery for enriched populations. Here, a reactor inoculated with water from Lake Fargette, France, and maintained under dark conditions at 4 C for 31 months enriched for diverse Planctomycetes and Candidate Phyla Radiation (CPR) bacteria. We reconstructed draft genomes and predicted metabolic traits for 12 diverse Planctomycetes and 9 CPR bacteria, some of which are likely representatives of undescribed families or genera. One CPR genome representing the little-studied lineage Peribacter (1.239 Mbp) was curated to completion, and unexpectedly, encodes the full gluconeogenesis pathway. Metatranscriptomic data indicate that some Planctomycetes and CPR bacteria were active under the culture conditions. We also reconstructed genomes and obtained transmission electron microscope images for numerous phages, including one with a >300 kbp genome and several predicted to infect Planctomycetes. Together, our analyses suggest that freshwater Planctomycetes may act as hubs for interaction networks that include symbiotic CPR bacteria and phages.

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