Three-dimensional neuron tracing from confocal microscope data using a head-coupled display

1994 ◽  
Author(s):  
Robert G. Scharein ◽  
Kellogg S. Booth ◽  
James J. Little
Keyword(s):  
Three Dimensional ◽  
Confocal Microscope ◽  
Neuron Tracing

Active and Passive Responses of Myofibroblasts in Response to Mechanical Stretching in 3D Culture

2008 ◽  
Author(s):  
Adam C. Nathan ◽  
Sheng-Lin Lee ◽  
J. Pablo Marquez ◽  
Tao Ju ◽  
Kenneth M. Pryse ◽  
...  
Keyword(s):  
Mechanical Testing ◽  
Cell Response ◽  
Three Dimensional ◽  
3D Culture ◽  
Confocal Microscope ◽  
Effective Resistance ◽  
Optical Measurements ◽  
Mechanical Stretching ◽  
Stretch Response ◽  
Cells Cultured

The stretch response of myofibroblast cells cultured in a three-dimensional environment was simultaneously measured and observed using a tissue stretching apparatus mounted to a confocal microscope. Optical measurements were used to assess mechanical estimates of cell response obtained through combined mechanical testing and biochemical inhibition. Results show that activated myofibroblasts provide an effective resistance on the order of 100s of kPa. In specific mechanical environments, stretched myofibroblasts release the ECM.

Confocal microscopy: Potential applications in micropaleontology

1996 ◽  
Vol 70 (6) ◽  
pp. 1084-1087 ◽  
Author(s):  
Harry Birkmann ◽  
Robert F. Lundin
Keyword(s):  
Confocal Microscopy ◽  
Size Range ◽  
Three Dimensional ◽  
Numerical Data ◽  
Confocal Microscope ◽  
Topographic Map ◽  
High Quality ◽  
Potential Applications

Preliminary experiments with a confocal microscope (Sarastro 2000®) demonstrate its value in micropaleontology for photography and three-dimensional morphometric studies. High-quality images of objects in the size range of 100 to 2,000 μm can be obtained without application of nonremovable coating. Images can easily be electronically converted to stereopairs or displayed as single images. Images are in focus throughout and are superior to light photographs. Numerical data (x, y, and z coordinates) in almost any desired amount can be extracted from enough points on the visible surface to provide for the construction of a detailed topographic map of the surface. All of this can be done without manipulating the specimen once it has been mounted.

scholarly journals Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration

1998 ◽  
Vol 84 (8) ◽  
pp. 4033-4042 ◽  
Author(s):  
M. Schrader ◽  
S. W. Hell ◽  
H. T. M. van der Voort
Keyword(s):  
Image Restoration ◽  
Three Dimensional ◽  
Super Resolution ◽  
Confocal Microscope
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