Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence

1993 ◽  
Author(s):  
Peter M. Goodwin ◽  
Charles W. Wilkerson, Jr. ◽  
W. Patrick Ambrose ◽  
Richard A. Keller
Keyword(s):  
Single Molecules ◽  
Laser Induced Fluorescence ◽  
Ultrasensitive Detection ◽  
Flowing Sample

Detection and lifetime measurement of single molecules in flowing sample streams by laser‐induced fluorescence

1993 ◽  
Vol 62 (17) ◽  
pp. 2030-2032 ◽  
Author(s):  
Charles W. Wilkerson ◽  
Peter M. Goodwin ◽  
W. Patrick Ambrose ◽  
John C. Martin ◽  
Richard A. Keller
Keyword(s):  
Single Molecules ◽  
Laser Induced Fluorescence ◽  
Lifetime Measurement ◽  
Flowing Sample

Ultrasensitive nuclease activity and inhibition assay using microchip electrophoresis with laser induced fluorescence detection

2016 ◽  
Vol 8 (8) ◽  
pp. 1852-1857 ◽  
Author(s):  
Yingfeng Qin ◽  
Jingjin Zhao ◽  
Yong Huang ◽  
Shuting Li ◽  
Shulin Zhao
Keyword(s):  
Fluorescence Detection ◽  
Nuclease Activity ◽  
Microchip Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Ultrasensitive Detection ◽  
Inhibition Assay ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method

A novel microchip electrophoresis method with laser induced fluorescence detection was developed for ultrasensitive detection of nuclease activity and inhibitors.

Single-Molecule Detection in the Near-IR Using Continuous-Wave Diode Laser Excitation with an Avalanche Photon Detector

1998 ◽  
Vol 52 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Steven A. Soper ◽  
Benjamin L. Legendre
Keyword(s):  
Diode Laser ◽  
Single Molecule ◽  
Continuous Wave ◽  
Single Photon ◽  
Single Molecules ◽  
Single Molecule Detection ◽  
Semiconductor Diode ◽  
Semiconductor Diode Laser ◽  
Near Ir ◽  
Flowing Sample

While single-molecule detection in flowing sample streams has been reported by a number of groups, the instrumentation can be somewhat prohibitive for many applications due to the complexity and extensive expertise required to operate such a device. In this paper we report on the construction of a single-molecule detection device that is rugged, compact, inexpensive, and easily operated by individuals not well trained in optics and laser operations. The single-molecule detection apparatus consists of a semiconductor diode laser operating in a continuous-wave (CW) mode and a single photon avalanche diode transducer for converting the detected photons into transistor–transistor logic (TTL) pulses for displaying the data. In addition, the sampling volume is produced by a single-component lens, to create a volume on the order of 1 pL, allowing the sampling of microliter volumes of material on reasonable time scales. The device is targeted for operation in the near-IR region (700–1000 nm), where matrix interferences are minimal. Our data will demonstrate the detection of single molecules for the near-IR dyes IR-132 and IR-125, in methanol solvents in flowing sample streams at sampling rates of 100–250 samples/s. Detection efficiencies for the investigated near-IR dyes were found to be 98% for IR-132 and 50% for IR-125. Previous attempts in our laboratory to detect single molecules of IR-125 using time-gated detection were unsuccessful because of the short upper-state lifetime of this fluorophore (τf = 472 ps).

Laser-induced fluorescence as an approach to single molecules detection in fluid solution

1997 ◽  
Author(s):  
W. Y. Ma ◽  
J. Xiong ◽  
K. L. Wen ◽  
J. Wei ◽  
L. Zhang ◽  
...  
Keyword(s):  
Single Molecules ◽  
Laser Induced Fluorescence ◽  
Fluid Solution

Detection of single molecules of phycoerythrin in hydrodynamically focused flows by laser-induced fluorescence

1987 ◽  
Vol 59 (17) ◽  
pp. 2158-2161 ◽  
Author(s):  
Dinh Cong Nguyen ◽  
Richard A. Keller ◽  
James H. Jett ◽  
John C. Martin
Keyword(s):  
Single Molecules ◽  
Laser Induced Fluorescence
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