scholarly journals In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling

2011 ◽  
Vol 16 (6) ◽  
pp. 067005 ◽  
Author(s):  
Xiaozhuo Shao ◽  
Wei Zheng ◽  
Zhiwei Huang
Keyword(s):  
Near Infrared ◽  
Autofluorescence Spectroscopy ◽  
In Vivo Diagnosis ◽  
Biochemical Modeling

Near-infrared autofluorescence spectroscopy for in vivo diagnosis of cervical precancer

2008 ◽  
Author(s):  
Jianhua Mo ◽  
Wei Zheng ◽  
Jeffrey Low ◽  
Joseph Ng ◽  
A. Ilancheran ◽  
...  
Keyword(s):  
Near Infrared ◽  
Autofluorescence Spectroscopy ◽  
Cervical Precancer ◽  
In Vivo Diagnosis

Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer

2011 ◽  
Vol 26 (10) ◽  
pp. 4104-4110 ◽  
Author(s):  
Mads Sylvest Bergholt ◽  
Wei Zheng ◽  
Kan Lin ◽  
Khek Yu Ho ◽  
Ming Teh ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Raman Spectroscopy ◽  
Near Infrared ◽  
In Vivo Diagnosis

scholarly journals Autofluorescence spectroscopy for in vivo diagnosis of DMBA-induced hamster buccal pouch pre-cancers and cancers

2003 ◽  
Vol 32 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Chih-Yu Wang ◽  
Tsuimin Tsai ◽  
Hui-Chun Chen ◽  
Shu-Chen Chang ◽  
Chin-Tin Chen ◽  
...  
Keyword(s):  
Hamster Buccal Pouch ◽  
Autofluorescence Spectroscopy ◽  
In Vivo Diagnosis

scholarly journals Near-infrared autofluorescence spectroscopy of in vivo soft tissue sarcomas

2015 ◽  
Vol 40 (23) ◽  
pp. 5498 ◽  
Author(s):  
John Quan Nguyen ◽  
Zain Gowani ◽  
Maggie O’Connor ◽  
Isaac Pence ◽  
The-Quyen Nguyen ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Near Infrared ◽  
Soft Tissue Sarcomas ◽  
Autofluorescence Spectroscopy

Near-infrared Raman spectroscopy for in-vivo diagnosis of cervical dysplasia: a probability-based multi-class diagnostic algorithm

2007 ◽  
Author(s):  
Shovan K. Majumder ◽  
Elizabeth Kanter ◽  
Amy Robichaux Viehoever ◽  
Howard Jones ◽  
Anita Mahadevan-Jansen
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Cervical Dysplasia ◽  
Diagnostic Algorithm ◽  
In Vivo Diagnosis

Near infrared spectroscopy and aquaphotomics: Novel approach for rapid in vivo diagnosis of virus infected soybean

2010 ◽  
Vol 397 (4) ◽  
pp. 685-690 ◽  
Author(s):  
Balasuriya Jinendra ◽  
Katsutomo Tamaki ◽  
Shinichiro Kuroki ◽  
Maria Vassileva ◽  
Shinya Yoshida ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Novel Approach ◽  
In Vivo Diagnosis

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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