scholarly journals Increase of reduced nicotinamide adenine dinucleotide fluorescence lifetime precedes mitochondrial dysfunction in staurosporine-induced apoptosis of HeLa cells

2011 ◽  
Vol 16 (3) ◽  
pp. 036008 ◽  
Author(s):  
Jia-Sin Yu ◽  
Han-Wen Guo ◽  
Chih-Hao Wang ◽  
Yau-Huei Wei ◽  
Hsing-Wen Wang
Keyword(s):  
Mitochondrial Dysfunction ◽  
Fluorescence Lifetime ◽  
Hela Cells ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine ◽  
Induced Apoptosis ◽  
Reduced Nicotinamide Adenine Dinucleotide

Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation

Blood ◽  
2003 ◽  
Vol 101 (1) ◽  
pp. 186-193 ◽  
Author(s):  
Elisabetta Ferrero ◽  
Daniela Belloni ◽  
Paola Contini ◽  
Chiara Foglieni ◽  
Maria Elena Ferrero ◽  
...  
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Critical Role ◽  
Cell Subset ◽  
Transendothelial Migration ◽  
Nicotinamide Adenine ◽  
Regulation Of Expression ◽  
Antiapoptotic Proteins ◽  
Platelet Endothelial Cell Adhesion ◽  
Induced Apoptosis ◽  
Reduced Nicotinamide Adenine Dinucleotide

Abstract In the present paper we show that transendothelial migration of a subset of CD14+ circulating leukocytes, coexpressing the CD34 precursor marker, leads to protection from the apoptosis that follows growth factor(s) withdrawal. The resistance of this cell subset to starvation-induced programmed cell death, lasting from 48 to 96 hours, is accompanied by a rise of mitochondrial adenosine triphosphate (ATP), a high nicotinamide adenine dinucleotide (NAD)/reduced nicotinamide adenine dinucleotide (NADH) ratio, and by the up-regulation of expression of the antiapoptotic proteins Bcl-2 and Bcl-X, together with an increase in the cytoplasmic, inactive, form of Bax. This suggests that protection from apoptosis is due to the preservation of mitochondrial function(s). Interestingly, ligation of the platelet endothelial cell adhesion molecule-1 (PECAM-1), which drives CD14+CD34+ transendothelial migration, leads to an increase in Bcl-2 A1 and Bcl-X intracellular content, and to protection from starvation-induced apoptosis. This event is dependent on the engagement of phosphatidylinositol-3 kinase and activation of Akt/PKB that is known to contribute to Bcl-2 and Bcl-X induction. These data point to a critical role of endothelium in preventing the apoptotic program triggered by starvation, possibly inducing a prolonged survival of antigen presenting cell precursors, in order to allow recirculation of these cells and localization to the site of priming of T lymphocytes.

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