scholarly journals Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

2010 ◽  
Vol 15 (6) ◽  
pp. 067005 ◽  
Author(s):  
Chun Wei Wang ◽  
Upender Manne ◽  
Vishnu B. Reddy ◽  
Denise K. Oelschlager ◽  
Venkat R. Katkoori ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Fiber Optic ◽  
Quantitative Estimation ◽  
Serum Samples ◽  
Tapered Fiber

scholarly journals Errata: Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

2011 ◽  
Vol 16 (1) ◽  
pp. 019801 ◽  
Author(s):  
Chun Wei Wang ◽  
Upender Manne ◽  
Vishnu B. Reddy ◽  
Denise K. Oelschlager ◽  
Venkat R. Katkoori ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Fiber Optic ◽  
Quantitative Estimation ◽  
Serum Samples ◽  
Tapered Fiber

Label-Free Thrombin Detection Using a Tapered Fiber-Optic Interferometric Aptasensor

2019 ◽  
Vol 37 (11) ◽  
pp. 2756-2761 ◽  
Author(s):  
Dandan Sun ◽  
Li-Peng Sun ◽  
Tuan Guo ◽  
Bai-Ou Guan
Keyword(s):  
Fiber Optic ◽  
Label Free ◽  
Tapered Fiber

scholarly journals A Paper-Based IL-6 Test Strip Coupled With a Spectrum-Based Optical Reader for Differentiating Influenza Severity in Children

2021 ◽  
Vol 9 ◽  
Author(s):  
Sheng-Wen Lin ◽  
Ching-Fen Shen ◽  
Ching-Chuan Liu ◽  
Chao-Min Cheng
Keyword(s):  
Patient Outcome ◽  
Influenza Virus ◽  
Early Diagnosis ◽  
Sensitivity And Specificity ◽  
Interleukin 6 ◽  
Test Strip ◽  
Assay Method ◽  
Virus Infections ◽  
Serum Samples ◽  
Optical Reader

Influenza virus infection is a major worldwide public health problem. Influenza virus infections are associated with a high hospitalization rate in children between the ages of 5 and 14. The predominant reason for poor influenza prognosis is the lack of any effective means for early diagnosis. Early diagnosis of severe illness is critical to improving patient outcome, and could be especially useful in areas with limited medical resources. Accurate, inexpensive, and easy-to-use diagnostic tools could improve early diagnosis and patient outcome, and reduce overall healthcare costs. We developed an interleukin-6 paper-based test strip that used colloidal gold-conjugated antibodies to detect human interleukin-6 protein. These complexes were captured on a paper-based test strip patterned with perpendicular T lines that were pre-coated with anti-human interleukin-6 antibodies. Applied serum samples interacted with these antibodies and presented as colored bands that could be read using a spectrum-based optical reader. The full-spectrum of the reflected light interleukin-6 protein signal could be obtained from the spectral optics module, and the standard could be used to quantitatively analyze interleukin 6 level in serum. We retrospectively evaluated 10 children (23 serum samples) with severe influenza virus infections, 26 children (26 serum samples) with mild influenza virus infections, and 10 healthy children (10 serum samples). Our system, the combined use of a paper-based test strip and a spectrum-based optical reader, provided both qualitative and quantitative information. When used with the optical reader, the detection limit was improved from a qualitative, naked-eye level of 400 pg/ml to a quantitative, optical reader level of 76.85 pg/ml. After monitoring serum interleukin-6 level via our system, we found a high correlation between our system results and those obtainable using a conventional sandwich enzyme-linked immunosorbent assay method (Rho = 0.706, p < 0.001). The sensitivity and specificity for differentiating between severe and mild influenza using our combined method (test strip coupled with optical reader) were 78.3 and 50.0%, respectively. When interleukin-6 was combined with serum C-reaction protein, the sensitivity and specificity were 85.7 and 95.5%, and the receiver operating characteristic area-under-the-curve was quite high (AUC = 0.911, p < 0.001). The potential advantages of our system, i.e., a paper-based test strip coupled with a spectrum-based optical reader, are as follows: 1) simple user operation; 2) rapid turnaround times–within 20 min; 3) high detection performance; and, 4) low-cost fabrication.

Monitoring of procalcitonin but not interleukin-6 is useful for the early prediction of anastomotic leakage after colorectal surgery

2017 ◽  
Vol 55 (7) ◽  
Author(s):  
Urszula Zielińska-Borkowska ◽  
Naser Dib ◽  
Wiesław Tarnowski ◽  
Tomasz Skirecki
Keyword(s):  
Colorectal Surgery ◽  
Anastomotic Leakage ◽  
Interleukin 6 ◽  
Early Recognition ◽  
Surgical Trauma ◽  
Serum Samples ◽  
Elective Colorectal Surgery ◽  
Surgical Infections ◽  
Bowel Diseases ◽  
The Difference

AbstractBackground:Early recognition of patients who have developed anastomotic leakage (AL) after colorectal surgery is crucial for the successful treatment of this complication. The aim of this study was to assess the usefulness of the assessment of procalcitonin (PCT) and interleukin-6 (IL-6) levels in the prognosis of AL.Methods:This observational study included 157 patients who underwent major elective colorectal surgery. The most common indications for surgery were cancer and inflammatory bowel diseases. Serum samples were obtained directly before surgery (D0) and 1 day (D1) after surgery, and the relationships between the serum concentrations of PCT and IL-6 and development of AL were assessed.Results:In total, 10.2% of patients developed post-surgical infections due to AL. PCT levels on D1 were significantly higher in patients who developed AL [2.73 (1.40–4.62)] than in those who recovered without complications [0.2 (0.09–0.44)]. The area under the ROC curve for PCT on D1 was 0.94, 95% CI (0.89–0.98). The sensitivity and specificity of the prediction of an infection were 87% and 87%, respectively, for PCT on D1, which was higher than 1.09 ng/mL. The increase in PCT concentration between D0 and D1 was significantly higher in patients with AL (p<0.001). Patients who developed AL had higher concentrations of IL-6 on D1, but the difference was not significant (p=0.28).Conclusions:This study confirms that surgical trauma increases serum PCT concentrations and that the concentration of PCT on D1 can predict AL after colorectal surgery. However, IL-6 is not a good early marker for developing AL.

scholarly journals Single mode tapered fiber-optic interferometer based refractive index sensor and its application to protein sensing

2014 ◽  
Vol 22 (19) ◽  
pp. 22802 ◽  
Author(s):  
T. K Yadav ◽  
R. Narayanaswamy ◽  
M. H. Abu Bakar ◽  
Y. Mustapha Kamil ◽  
M. A. Mahdi
Keyword(s):  
Refractive Index ◽  
Fiber Optic ◽  
Single Mode ◽  
Refractive Index Sensor ◽  
Protein Sensing ◽  
Tapered Fiber ◽  
Fiber Optic Interferometer

scholarly journals Longitudinal Relationship Between Interleukin-6 and Perceived Fatigability Among Well-Functioning Adults in Mid-to-Late Life

2018 ◽  
Vol 74 (5) ◽  
pp. 720-725 ◽  
Author(s):  
Amal A Wanigatunga ◽  
Ravi Varadhan ◽  
Eleanor M Simonsick ◽  
Olga D Carlson ◽  
Stephanie Studenski ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Repeated Measures ◽  
Perceived Exertion ◽  
Functional Decline ◽  
Annual Change ◽  
Serum Samples ◽  
Time Interaction ◽  
Physiological Dysregulation ◽  
Longitudinal Association ◽  
Baltimore Longitudinal Study

Abstract Background Chronically elevated interleukin-6 (IL-6) levels contribute to fatigue and functional decline via multiple pathways that often lead to frailty. Lesser known is the contribution of IL-6 to fatigue in relation to a standardized workload (fatigability), a precursor to functional decline. Therefore, the purpose of this study was to examine the longitudinal relationship between IL-6 and fatigability. Methods About 985 participants from the Baltimore Longitudinal Study of Aging (mean age: 70 ± 10 years) were evaluated every 1–4 years. IL-6 was measured in fasting serum samples at each visit and log-transformed for analyses. Perceived fatigability (PF) was defined as self-reported exertion (rate of perceived exertion; RPE) after a 5-min, 0.67 m/s, 0% grade treadmill walk. Continuous and categorical associations between IL-6 (baseline and repeated measures) and PF were assessed using generalized estimating equations, adjusting for demographics, behavioral factors, and comorbid conditions. Results In fully adjusted continuous models, twofold higher baseline IL-6 was associated with a 0.28 higher RPE (p = .03). This relationship tended to remain constant annually (baseline log IL-6 by time interaction p = .29). To provide clinical relevance, the sample median (3.7 pg/mL) was used to examine high versus low IL-6 levels. Over time, the high group reported an average 0.25 higher RPE (p = .03) than the low group. Annual change in logged IL-6 was not associated with annual change in PF (p = .48). Conclusion Findings suggest that elevated IL-6 is a biomarker of physiological dysregulation associated with greater fatigability, but there is no longitudinal association between IL-6 and fatigability. Future studies should evaluate whether interventions that aim to reduce inflammation also attenuate fatigability.

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