scholarly journals Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

2010 ◽  
Vol 15 (2) ◽  
pp. 020506 ◽  
Author(s):  
YongKeun Park ◽  
Monica Diez-Silva ◽  
Dan Fu ◽  
Gabriel Popescu ◽  
Wonshik Choi ◽  
...  
PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0176799 ◽  
Author(s):  
Johannes Mauer ◽  
Matti Peltomäki ◽  
Simón Poblete ◽  
Gerhard Gompper ◽  
Dmitry A. Fedosov

2017 ◽  
Vol 11 (2) ◽  
pp. e201700070 ◽  
Author(s):  
Jianbo Tang ◽  
Sefik Evren Erdener ◽  
Baoqiang Li ◽  
Buyin Fu ◽  
Sava Sakadzic ◽  
...  

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38999 ◽  
Author(s):  
Andreas Greth ◽  
Shelley Lampkin ◽  
Preethi Mayura-Guru ◽  
Fleur Rodda ◽  
Karen Drysdale ◽  
...  

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.


Author(s):  
Adil Raza ◽  
Megha Chaudhary ◽  
Sonika Devi

Background: Malaria is a systematic disease caused by a parasite called Plasmodium which is transmitted into the human blood via female Anopheles mosquito. Malaria in humans is caused by four species of protozoan parasites of the genus Plasmodium: P. falciparum, P. vivax, P. ovale, and P. malariae. The parasite enters the human body through a mosquito bite and travel to the very crucial organ, the liver, where they multiply and come back to the bloodstream and destroy red blood cells. Malaria causes symptoms that typically include fever, tiredness, vomiting, and headaches. In severe cases it can cause yellow skin, seizures, coma, or death. Symptoms usually begin ten to fifteen days after being bitten by an infected mosquito. In those who have recently survived an infection, reinfection usually causes milder symptoms. Objectives: Isolation of different species of malaria parasites. The prevalence of malaria parasite in India. Methods: The procedure follows these steps: collection of peripheral blood, staining of smear with Leishman’s stain and examination of red blood cells for malaria parasites under the microscope. Results: We observed the plasmodium species in peripheral blood smear. Conclusion: Worldwide, the number of cases of malaria caused by Plasmodium falciparum, the most dangerous species of the parasite, is on the rise.


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