scholarly journals In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues

2009 ◽  
Vol 14 (2) ◽  
pp. 021006 ◽  
Author(s):  
Alexandru S. Biris ◽  
Ekaterina I. Galanzha ◽  
Zhongrui Li ◽  
Meena Mahmood ◽  
Yang Xu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Carbon Nanotube ◽  
Real Time ◽  
Real Time Detection

scholarly journals Real-time Detection of Circulating Apoptotic Cells by in Vivo Flow Cytometry

2005 ◽  
Vol 4 (4) ◽  
pp. 7290.2005.05148 ◽  
Author(s):  
Xunbin Wei ◽  
Dorothy A. Sipkins ◽  
Costas M. Pitsillides ◽  
John Novak ◽  
Irene Georgakoudi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Apoptotic Cells ◽  
Real Time Detection ◽  
In Vivo Flow Cytometry

Photoacoustic flow cytometry: principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo

2007 ◽  
Vol 12 (5) ◽  
pp. 051503 ◽  
Author(s):  
Vladimir P. Zharov ◽  
Ekaterina I. Galanzha ◽  
Evgeny V. Shashkov ◽  
Jin-Woo Kim ◽  
Nikolai G. Khlebtsov ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Real Time Detection

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

Head & Neck ◽  
2013 ◽  
Vol 36 (8) ◽  
pp. 1207-1215 ◽  
Author(s):  
Mazen A. Juratli ◽  
Mustafa Sarimollaoglu ◽  
Eric R. Siegel ◽  
Dmitry A. Nedosekin ◽  
Ekaterina I. Galanzha ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
Cell Release

A reversible near-infrared fluorescence probe for the monitoring of HSO3–/H2O2 regulated cycles in vivo

2021 ◽  
Author(s):  
Qiuying Song ◽  
Bo Zhou ◽  
Dongyu Zhang ◽  
Haijun Chi ◽  
Hongmin Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Fluorescence Probe ◽  
Redox Homeostasis ◽  
Research Field ◽  
Fluorescence Probes ◽  
Detection Capability ◽  
Near Infrared Fluorescence ◽  
Real Time Detection

The development of well-designed fluorescence probes for the monitoring redox homeostasis in biosystems has become a desired research field owing to their noninvasive and real-time detection capability in vivo. In...

Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes

2013 ◽  
Vol 25 (7) ◽  
pp. 1757-1763 ◽  
Author(s):  
Nuno R. Ferreira ◽  
Ricardo M. Santos ◽  
João Laranjinha ◽  
Rui M. Barbosa
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
In Vivo Measurement ◽  
The Brain

Long-Term Increase in Fetal Hemoglobin Expression in Nonhuman Primates Following Transplantation of Autologous Bcl11a Nuclease-Edited HSCs

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 2035-2035 ◽  
Author(s):  
Olivier Humbert ◽  
Hans-Peter Kiem
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Nonhuman Primates ◽  
Pcr Analysis ◽  
Beta Globin ◽  
Gamma Globin ◽  
Cd34 Cells

Abstract Elevated levels of fetal hemoglobin (HbF) ameliorate the clinical symptoms of beta-thalassemia and sickle cell anemia. The transcription factor B-cell lymphoma/leukemia 11A (BCL11A) is required for silencing of gamma-globin expression in adult erythroid cells and functions as a switch from fetal to adult hemoglobin production in humans. BCL11A therefore constitutes a therapeutic target for the treatment of hemoglobinopathies. We inactivated BCL11A function by double-strand DNA break-induced mutagenesis using Transcription Activator-Like Effector Nucleases (TALENs). 20 to 30% gene editing could be achieved in vitro in human and nonhuman primate CD34+ cells by TALEN mRNAs electroporation targeting exon 2 of Bcl11a. Colony-forming efficiency was slightly lower in Bcl11a-edited CD34+ cells but lineage differentiation potential was unchanged. Erythroid differentiation of CD34+ cells in culture showed increased Fetal to Beta hemoglobin ratio in both human and primate Bcl11a-modified cells as compared to control cells, thus validating our editing approach to increase HbF production. To determine if Bcl11a-edited hematopoietic stem cells (HSCs) could be engrafted and give rise to HbF-producing erythrocytes, we transplanted a pigtail macaque with autologous CD34+ electroporated with Bcl11a TALEN mRNA following conditioning by total body irradiation. We detected about 1 % gene disruption in vivo early post-transplant and disruption frequency gradually declined to reach a set point of about 0.3% starting at day 28 post-transplantation. In this analysis, which we have so far taken out to 42 days, single clones could be tracked based on their mutation signature, and we found that several clones persisted over time, confirming engraftment of Bcl11a-modified cells. Since the transplantation procedure and chemo-radiotherapy conditioning can raise HbF production, three control animals that were transplanted using similar conditions as with the Bcl11a-edited HSCs and one untransplanted animal were also included in our analysis. Flow cytometry measurement of HbF in peripheral blood showed a rapid increase in F-cell production in all animals, reaching levels that ranged from 10% to 40% by 30 days, while the untransplanted control showed basal HbF expression of about 0.5% (Fig. 1A). The peak for HbF expression lasted for about 140 days and eventually returned to basal levels that averaged 0.5% for all control animals. In comparison, the animal transplanted with Bcl11a-edited cells showed significantly higher HbF levels starting at day 140 post-treatment (1-1.5%), and HbF production has remained constant for at least 150 days. This result was confirmed by hemoglobin mRNA analysis in peripheral blood using real-time PCR. We found a rapid increase in gamma globin expression following transplantation, before returning to near basal levels. As compared to controls, the animal transplanted with Bcl11a-edited cells showed a 5 to 10-fold increase in gamma to beta globin ratio at day 140 and this ratio has remained constant ever since (Fig. 1B). We are currently working on ways to enhance Bcl11a-editing and to select for Bcl11a-modified HSCs using targeted integration of the chemoselection cassette P140K MGMT to ultimately achieve curative HbF production. Potential TALEN off-target sites will also be examined as well as any side effect associated with the inactivation of BCL11A. Overall, our data demonstrate that transplantation of Bcl11a-edited HSCs results in elevated HbF production in nonhuman primates. Furthermore, we show that nonhuman primates can serve as a useful model for novel gene editing strategies toward the treatment of hemoglobinopathies. Figure 1. In vivo monitoring of HbF expression by flow cytometry and real-time PCR. (A) Intracellular HbF staining of peripheral blood measured by flow cytometry. (B) Real-time PCR analysis of hemoglobin transcripts in RNA isolated from peripheral blood. Expression was normalized to GAPDH and %HbG is calculated as HbG/(HbG+HbB). HbG=gamma globin; HbB=beta globin. Black line=Bcl11a transplant; grey line=control transplant; dashed line=untransplanted control. Figure 1. In vivo monitoring of HbF expression by flow cytometry and real-time PCR. (A) Intracellular HbF staining of peripheral blood measured by flow cytometry. (B) Real-time PCR analysis of hemoglobin transcripts in RNA isolated from peripheral blood. Expression was normalized to GAPDH and %HbG is calculated as HbG/(HbG+HbB). HbG=gamma globin; HbB=beta globin. Black line=Bcl11a transplant; grey line=control transplant; dashed line=untransplanted control. Disclosures No relevant conflicts of interest to declare.

Simulated Real-Time Detection of a Small Molecule on a Carbon Nanotube Cantilever

2011 ◽  
Vol 8 (8) ◽  
pp. 1364-1384 ◽  
Author(s):  
Reese E. Jones ◽  
Jeremy A. Templeton ◽  
Timothy W. Rebold
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Small Molecule ◽  
Real Time Detection
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