scholarly journals Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats

2008 ◽  
Vol 13 (6) ◽  
pp. 064035 ◽  
Author(s):  
Seonkyung Lee ◽  
Danthu H. Vu ◽  
Michael F. Hinds ◽  
Steven J. Davis ◽  
Alvin Liang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Diode Laser ◽  
In Vivo Studies ◽  
Oxygen Monitor

scholarly journals Errata: Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats

2009 ◽  
Vol 14 (1) ◽  
pp. 019801
Author(s):  
Seonkyung Lee ◽  
Danthu H. Vu ◽  
Michael F. Hinds ◽  
Steven J. Davis ◽  
Alvin Liang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Diode Laser ◽  
In Vivo Studies ◽  
Oxygen Monitor

A Diode Laser-Based Singlet Oxygen Monitor for Photodynamic Therapy; in- vitro and in- vivo Studies

2006 ◽  
Author(s):  
Seonkyung Lee ◽  
Danthu H. Vu ◽  
Michael F. Hinds ◽  
Steven J. Davis ◽  
Tayyaba Hasan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Diode Laser ◽  
In Vivo Studies ◽  
Oxygen Monitor

A singlet oxygen monitor as an in vivo photodynamic therapy dosimeter

2009 ◽  
Author(s):  
S. Lee ◽  
K. Galbally-Kinney ◽  
M. F. Hinds ◽  
J. A. O'Hara ◽  
B. W. Pogue ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Oxygen Monitor

scholarly journals Heterometallic Ru–Pt metallacycle for two-photon photodynamic therapy

2018 ◽  
Vol 115 (22) ◽  
pp. 5664-5669 ◽  
Author(s):  
Zhixuan Zhou ◽  
Jiangping Liu ◽  
Thomas W. Rees ◽  
Heng Wang ◽  
Xiaopeng Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Near Infrared ◽  
Photon Absorption ◽  
In Vivo Studies ◽  
Target Tissue ◽  
Noninvasive Treatment ◽  
Two Photon Absorption ◽  
Two Photon

As an effective and noninvasive treatment of various diseases, photodynamic therapy (PTD) relies on the combination of light, a photosensitizer, and oxygen to generate cytotoxic reactive oxygen species that can damage malignant tissue. Much attention has been paid to covalent modifications of the photosensitizers to improve their photophysical properties and to optimize the pathway of the photosensitizers interacting with cells within the target tissue. Herein we report the design and synthesis of a supramolecular heterometallic Ru–Pt metallacycle via coordination-driven self-assembly. While inheriting the excellent photostability and two-photon absorption characteristics of the Ru(II) polypyridyl precursor, the metallacycle also exhibits red-shifted luminescence to the near-infrared region, a larger two-photon absorption cross-section, and higher singlet oxygen generation efficiency, making it an excellent candidate as a photosensitizer for PTD. Cellular studies reveal that the metallacycle selectively accumulates in mitochondria and nuclei upon internalization. As a result, singlet oxygen generated by photoexcitation of the metallacycle can efficiently trigger cell death via the simultaneous damage to mitochondrial function and intranuclear DNA. In vivo studies on tumor-bearing mice show that the metallacycle can efficiently inhibit tumor growth under a low light dose with minimal side effects. The supramolecular approach presented in this work provides a paradigm for the development of PDT agents with high efficacy.

Linear and high-molecular-weight poly-porphyrins for efficient photodynamic therapy

2021 ◽  
Author(s):  
Nan Zheng ◽  
Xiahui Li ◽  
Shangwei Huangfu ◽  
Kangkai Xia ◽  
Ruofei Yue ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Photodynamic Therapy ◽  
Quantum Yield ◽  
Singlet Oxygen ◽  
High Molecular Weight ◽  
Linear Poly ◽  
High Molecular Weight Poly

A linear poly-porphyrin with high Mw and conjugated by PEG and acetazolamide was developed with enhanced singlet oxygen quantum yield, improved photo-toxicity and excellent in vivo photodynamic therapy.

scholarly journals A self-assembled Ru–Pt metallacage as a lysosome-targeting photosensitizer for 2-photon photodynamic therapy

2019 ◽  
Vol 116 (41) ◽  
pp. 20296-20302 ◽  
Author(s):  
Zhixuan Zhou ◽  
Jiangping Liu ◽  
Juanjuan Huang ◽  
Thomas W. Rees ◽  
Yiliang Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Building Blocks ◽  
Cell Uptake ◽  
Photon Absorption ◽  
In Vivo Studies ◽  
Infrared Light ◽  
Ros Generation ◽  
Multicellular Spheroids

Photodynamic therapy (PDT) is a treatment procedure that relies on cytotoxic reactive oxygen species (ROS) generated by the light activation of a photosensitizer. The photophysical and biological properties of photosensitizers are vital for the therapeutic outcome of PDT. In this work a 2D rhomboidal metallacycle and a 3D octahedral metallacage were designed and synthesized via the coordination-driven self-assembly of a Ru(II)-based photosensitizer and complementary Pt(II)-based building blocks. The metallacage showed deep-red luminescence, a large 2-photon absorption cross-section, and highly efficient ROS generation. The metallacage was encapsulated into an amphiphilic block copolymer to form nanoparticles to encourage cell uptake and localization. Upon internalization into cells, the nanoparticles selectively accumulate in the lysosomes, a favorable location for PDT. The nanoparticles are almost nontoxic in the dark, and can efficiently destroy tumor cells via the generation of ROS in the lysosomes under 2-photon near-infrared light irradiation. The superb PDT efficacy of the metallacage-containing nanoparticles was further validated by studies on 3D multicellular spheroids (MCS) and in vivo studies on A549 tumor-bearing mice.

939 TBr4 and BBr2 as Sensitizers for Photodynamic Therapy Against Human Colorectal Adenocarcinoma – in Vivo Studies

2012 ◽  
Vol 48 ◽  
pp. S226
Author(s):  
M. Laranjo ◽  
A.M. Abrantes ◽  
A.C. Serra ◽  
M. Pineiro ◽  
L. Carvalho ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Colorectal Adenocarcinoma ◽  
In Vivo Studies

Molecular Basis of the Vulnerability of Photosystem II to Damage by Light

1995 ◽  
Vol 22 (2) ◽  
pp. 201 ◽  
Author(s):  
J Barber
Keyword(s):  
Triplet State ◽  
Singlet Oxygen ◽  
D1 Protein ◽  
Primary Electron ◽  
In Vivo Studies ◽  
Cytochrome B559 ◽  
Acceptor Side ◽  
Donor And Acceptor ◽  
Primary Cleavage

Using isolated reaction centres and cores of photosystem I1 (PSII) it has been possible to elucidate the details of two separate pathways which lead to photoinhibition. The acceptor side pathway involves charge recombination resulting in the formation of the triplet state of the primary electron donor, P680. This triplet state is harmless in the absence of oxygen but in its presence gives rise to highly reactive singlet oxygen. We have shown that this singlet oxygen specifically attacks the chlorophyll of P680 itself. This process, plus other possibilities, gives rise to degradation of Dl protein involving a primary cleavage in the stromal loop joining putative transmembrane regions four and five, to yield 23 kDa N-terminal and 10 kDa C-terminal fragments. In contrast a donor side pathway is oxygen independent and is due to detrimental secondary oxidations brought about by P680+. Oxidation of accessory chlorophyll (C670) and β-carotene are observed and D1 protein is degraded by a primary cleavage in the lumenal loop between the putative transmembrane segments one and two to yield 24 kDa C-terminal and 9 kDa N-terminal fragments. In vivo studies indicate that the acceptor pathway is more common. The reason for the inherent vulnerability of PSII to photoinduced damage is discussed in terms of the special nature of P68O and the implications of the role of cytochrome b559 as a versatile protectant against donor and acceptor side photoinactivation is also considered. The likely dimeric organisation of PSII in vivo adds an additional factor to the general discussion of the molecular processes which underlie the vulnerability of PSII to photoinduced damage.

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