Parallel two-channel near- and far-field fluorescence microscopy

2007 ◽  
Vol 12 (3) ◽  
pp. 034012 ◽  
Author(s):  
Dorinel Verdes ◽  
Thomas Ruckstuhl ◽  
Stefan Seeger
Keyword(s):  
Fluorescence Microscopy ◽  
Far Field

Two-Point Separation in Far-Field Super-Resolution Fluorescence Microscopy Based on Two-Color Fluorescence Dip Spectroscopy, Part I: Experimental Evaluation

2005 ◽  
Vol 59 (7) ◽  
pp. 868-872 ◽  
Author(s):  
Takeshi Watanabe ◽  
Yoshinori Iketaki ◽  
Takashige Omatsu ◽  
Kimihisa Yamamoto ◽  
Masaaki Fujii
Keyword(s):  
Fluorescence Microscopy ◽  
Phase Modulation ◽  
Laser Light ◽  
Super Resolution ◽  
Separation Distance ◽  
Diffraction Limit ◽  
Far Field ◽  
Fluorescent Beads ◽  
Fluorescent Image ◽  
Point Separation

The two-point resolution of a novel two-color far-field super-resolution fluorescence microscopy was evaluated by measuring fluorescent beads 100 nm in diameter. This microscopy is based on a combination of two-color fluorescence dip spectroscopy and a phase-modulation technique for a laser beam. By simply introducing two-color laser light, the size of the fluorescent image of a bead was shrunk down to a diameter of 250 nm from the diffraction-limited image with a diameter of 360 nm. For two closely adjacent fluorescent beads with a separation distance of 350 nm, the two-color microscope clearly gave separated fluorescence images, while the conventional one-color fluorescence microscope could not resolve them. It has been proved that our technique breaks Rayleigh's diffraction limit.

scholarly journals Molecular Orientation Affects Localization Accuracy in Superresolution Far-Field Fluorescence Microscopy

Nano Letters ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 209-213 ◽  
Author(s):  
Johann Engelhardt ◽  
Jan Keller ◽  
Patrick Hoyer ◽  
Matthias Reuss ◽  
Thorsten Staudt ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Molecular Orientation ◽  
Far Field ◽  
Localization Accuracy

Far-field fluorescence microscopy at the macromolecular scale

2006 ◽  
Author(s):  
S. W. Hell ◽  
K. Willig ◽  
M. Hofmann ◽  
C. Eggeling ◽  
V. Westphal
Keyword(s):  
Fluorescence Microscopy ◽  
Far Field

scholarly journals Simultaneous near-field and far-field fluorescence microscopy of single molecules

2011 ◽  
Vol 19 (7) ◽  
pp. 6836 ◽  
Author(s):  
Thomas Ruckstuhl ◽  
Dorinel Verdes ◽  
Christian M. Winterflood ◽  
Stefan Seeger
Keyword(s):  
Fluorescence Microscopy ◽  
Near Field ◽  
Single Molecules ◽  
Far Field

Far-field fluorescence microscopy beyond the diffraction limit

1999 ◽  
Vol 16 (4) ◽  
pp. 909 ◽  
Author(s):  
A. M. van Oijen ◽  
J. Köhler ◽  
J. Schmidt ◽  
M. Müller ◽  
G. J. Brakenhoff
Keyword(s):  
Fluorescence Microscopy ◽  
Diffraction Limit ◽  
Far Field

Far-field fluorescence microscopy with repetitive excitation

1999 ◽  
Vol 6 (3) ◽  
pp. 283-290 ◽  
Author(s):  
A. Schönle ◽  
S.W. Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Far Field

scholarly journals Two-Point-Separation in Far-Field Super-Resolution Fluorescence Microscopy Based on Two-Color Fluorescence Dip Spectroscopy

Hyomen Kagaku ◽  
2004 ◽  
Vol 25 (8) ◽  
pp. 466-472
Author(s):  
Takeshi WATANABE ◽  
Yoshinori IKETAKI ◽  
Takashige OMATSU ◽  
Kimihisa YAMAMOTO ◽  
Masaaki FUJII
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Far Field ◽  
Point Separation
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