Method used to measure interaction of proteins with dual-beam optical tweezers

2006 ◽  
Vol 11 (6) ◽  
pp. 064035 ◽  
Author(s):  
E. Qu ◽  
Honglian Guo ◽  
Chunhua Xu ◽  
Chunxiang Liu ◽  
Zhaolin Li ◽  
...  
Keyword(s):  
1999 ◽  
Vol 39 (supplement) ◽  
pp. S94
Author(s):  
M. Tomishige ◽  
S. Manno ◽  
Y. Takakuwa ◽  
A. Kusumi
Keyword(s):  

2016 ◽  
Vol 18 (37) ◽  
pp. 25823-25830 ◽  
Author(s):  
Dipankar Mondal ◽  
Paresh Mathur ◽  
Debabrata Goswami

We present a novel method of microrheology based on femtosecond optical tweezers, which in turn enables us to directly measure and controlin situtemperature at microscale volumes at the solid–liquid interface.


2014 ◽  
Vol 85 (5) ◽  
pp. 053108 ◽  
Author(s):  
Dino Ott ◽  
S. Nader S. Reihani ◽  
Lene B. Oddershede

2017 ◽  
Vol 114 (5) ◽  
pp. 1015-1020 ◽  
Author(s):  
Marco Grison ◽  
Ulrich Merkel ◽  
Julius Kostan ◽  
Kristina Djinović-Carugo ◽  
Matthias Rief

Stable anchoring of titin within the muscle Z-disk is essential for preserving muscle integrity during passive stretching. One of the main candidates for anchoring titin in the Z-disk is the actin cross-linker α-actinin. The calmodulin-like domain of α-actinin binds to the Z-repeats of titin. However, the mechanical and kinetic properties of this important interaction are still unknown. Here, we use a dual-beam optical tweezers assay to study the mechanics of this interaction at the single-molecule level. A single interaction of α-actinin and titin turns out to be surprisingly weak if force is applied. Depending on the direction of force application, the unbinding forces can more than triple. Our results suggest a model where multiple α-actinin/Z-repeat interactions cooperate to ensure long-term stable titin anchoring while allowing the individual components to exchange dynamically.


2021 ◽  
Author(s):  
Tengfang Kuang ◽  
Zijie Liu ◽  
WEI XIONG ◽  
Xiang Han ◽  
Guangzong Xiao ◽  
...  

2006 ◽  
Author(s):  
John P. Rickgauer ◽  
Derek N. Fuller ◽  
Douglas E. Smith

2005 ◽  
Author(s):  
Gregor G. Knoener ◽  
Simon J. W. Parkin ◽  
Norman R. Heckenberg ◽  
Halina Rubinsztein-Dunlop

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