Segmentation method for three-dimensional visualization of microscopic objects imaged with a confocal laser scanning microscope

2006 ◽  
Vol 15 (4) ◽  
pp. 043005 ◽  
Author(s):  
Jeffrey R. Anderson
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Segmentation Method ◽  
Scanning Microscope ◽  
Laser Scanning Microscope

Three-dimensional microscopy using a confocal laser scanning microscope

1985 ◽  
Vol 10 (2) ◽  
pp. 53 ◽  
Author(s):  
K. Carlsson ◽  
P. E. Danielsson ◽  
A. Liljeborg ◽  
L. Majlöf ◽  
R. Lenz ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

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