In vivo determination of optical properties of normal and tumor tissue with white light reflectance and an empirical light transport model during endoscopy

2005 ◽  
Vol 10 (3) ◽  
pp. 034018 ◽  
Author(s):  
P. R. Bargo ◽  
S. A. Prahl ◽  
T. T. Goodell ◽  
R. A. Sleven ◽  
G. Koval ◽  
...  
Keyword(s):  
Optical Properties ◽  
White Light ◽  
Tumor Tissue ◽  
Transport Model ◽  
Light Transport ◽  
Light Reflectance

scholarly journals Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 153
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Ioannis Raptis ◽  
Panagiota Petrou ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
White Light ◽  
Optical Biosensor ◽  
Reflectance Spectroscopy ◽  
Fruit Juices ◽  
Sample Treatment ◽  
Assay Sensitivity ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

Fast, sensitive and selective determination of herbicide glyphosate in water samples with a White Light Reflectance Spectroscopy immunosensor

Talanta ◽  
2020 ◽  
Vol 214 ◽  
pp. 120854 ◽  
Author(s):  
Eleftheria Stavra ◽  
Panagiota S. Petrou ◽  
Georgios Koukouvinos ◽  
Anastasios Economou ◽  
Dimitris Goustouridis ◽  
...  
Keyword(s):  
White Light ◽  
Water Samples ◽  
Reflectance Spectroscopy ◽  
Selective Determination ◽  
Light Reflectance ◽  
Herbicide Glyphosate

In vivo determination of 5-bromo-2′-deoxyuridine incorporation into DNA tumor tissue by a new 32P-postlabelling thin-layer chromatographic method

1997 ◽  
Vol 694 (2) ◽  
pp. 333-341 ◽  
Author(s):  
Jacob J Steinberg ◽  
Gary W Oliver ◽  
Nazih Farah ◽  
Payman Simoni ◽  
Raz Winiarsky ◽  
...  
Keyword(s):  
Thin Layer ◽  
Chromatographic Method ◽  
Tumor Tissue

Rapid and sensitive label-free determination of aflatoxin M1 levels in milk through a White Light Reflectance Spectroscopy immunosensor

2019 ◽  
Vol 282 ◽  
pp. 104-111 ◽  
Author(s):  
Dimitra Tsounidi ◽  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Konstantinos Misiakos ◽  
Grigorios Zisis ◽  
...  
Keyword(s):  
White Light ◽  
Reflectance Spectroscopy ◽  
Aflatoxin M1 ◽  
Label Free ◽  
Light Reflectance
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