Parallel single molecule detection with a fully integrated single-photon 2×2 CMOS detector array

2004 ◽  
Vol 9 (5) ◽  
pp. 913 ◽  
Author(s):  
Michael Gösch ◽  
Alexandre Serov ◽  
Tiemo Anhut ◽  
Theo Lasser ◽  
Alexis Rochas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Photon ◽  
Single Molecule Detection ◽  
Detector Array ◽  
Fully Integrated

Toward single-molecule detection with very compact DNA sequencer based on single-photon avalanche diode array

2008 ◽  
Author(s):  
Ivan Rech ◽  
Stefano Marangoni ◽  
Angelo Gulinatti ◽  
Massimo Ghioni ◽  
Sergio Cova
Keyword(s):  
Single Molecule ◽  
Single Photon ◽  
Single Molecule Detection ◽  
Diode Array ◽  
Avalanche Diode ◽  
Dna Sequencer

Single-Molecule Detection in the Near-IR Using Continuous-Wave Diode Laser Excitation with an Avalanche Photon Detector

1998 ◽  
Vol 52 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Steven A. Soper ◽  
Benjamin L. Legendre
Keyword(s):  
Diode Laser ◽  
Single Molecule ◽  
Continuous Wave ◽  
Single Photon ◽  
Single Molecules ◽  
Single Molecule Detection ◽  
Semiconductor Diode ◽  
Semiconductor Diode Laser ◽  
Near Ir ◽  
Flowing Sample

While single-molecule detection in flowing sample streams has been reported by a number of groups, the instrumentation can be somewhat prohibitive for many applications due to the complexity and extensive expertise required to operate such a device. In this paper we report on the construction of a single-molecule detection device that is rugged, compact, inexpensive, and easily operated by individuals not well trained in optics and laser operations. The single-molecule detection apparatus consists of a semiconductor diode laser operating in a continuous-wave (CW) mode and a single photon avalanche diode transducer for converting the detected photons into transistor–transistor logic (TTL) pulses for displaying the data. In addition, the sampling volume is produced by a single-component lens, to create a volume on the order of 1 pL, allowing the sampling of microliter volumes of material on reasonable time scales. The device is targeted for operation in the near-IR region (700–1000 nm), where matrix interferences are minimal. Our data will demonstrate the detection of single molecules for the near-IR dyes IR-132 and IR-125, in methanol solvents in flowing sample streams at sampling rates of 100–250 samples/s. Detection efficiencies for the investigated near-IR dyes were found to be 98% for IR-132 and 50% for IR-125. Previous attempts in our laboratory to detect single molecules of IR-125 using time-gated detection were unsuccessful because of the short upper-state lifetime of this fluorophore (τf = 472 ps).

scholarly journals Single-molecule detection on a portable 3D-printed microscope

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
James W. P. Brown ◽  
Arnaud Bauer ◽  
Mark E Polinkovsky ◽  
Akshay Bhumkar ◽  
Dominic J. B. Hunter ◽  
...  
Keyword(s):  
Single Molecule ◽  
Amyloid Fibrils ◽  
Single Photon ◽  
Photon Counting ◽  
Correlation Spectroscopy ◽  
Single Molecule Detection ◽  
Single Photon Counting ◽  
Fluorescence Correlation ◽  
3D Printed ◽  
Simple Format

AbstractSingle-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and fluorescence correlation spectroscopy (FCS) in a simple format and is widely applicable to the detection of single fluorophores, proteins, liposomes or bacteria. The power of single-molecule detection is demonstrated by detecting single α-synuclein amyloid fibrils, that are currently evaluated as biomarkers for Parkinson’s disease, with an improved sensitivity of >100,000-fold over bulk measurements.

scholarly journals Noise and Breakdown Characterization of SPAD Detectors with Time-Gated Photon-Counting Operation

Sensors ◽  
2021 ◽  
Vol 21 (16) ◽  
pp. 5287
Author(s):  
Hiwa Mahmoudi ◽  
Michael Hofbauer ◽  
Bernhard Goll ◽  
Horst Zimmermann
Keyword(s):  
Breakdown Voltage ◽  
Single Photon ◽  
Photon Counting ◽  
Low Noise ◽  
Dark Count ◽  
Fully Integrated ◽  
Trade Offs ◽  
And Performance ◽  
Noise Models

Being ready-to-detect over a certain portion of time makes the time-gated single-photon avalanche diode (SPAD) an attractive candidate for low-noise photon-counting applications. A careful SPAD noise and performance characterization, however, is critical to avoid time-consuming experimental optimization and redesign iterations for such applications. Here, we present an extensive empirical study of the breakdown voltage, as well as the dark-count and afterpulsing noise mechanisms for a fully integrated time-gated SPAD detector in 0.35-μm CMOS based on experimental data acquired in a dark condition. An “effective” SPAD breakdown voltage is introduced to enable efficient characterization and modeling of the dark-count and afterpulsing probabilities with respect to the excess bias voltage and the gating duration time. The presented breakdown and noise models will allow for accurate modeling and optimization of SPAD-based detector designs, where the SPAD noise can impose severe trade-offs with speed and sensitivity as is shown via an example.

scholarly journals A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases

2021 ◽  
Author(s):  
Li-juan Wang ◽  
Le Liang ◽  
Bing-jie Liu ◽  
BingHua Jiang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Amplification Cascade

A controlled T7 transcription-driven symmetric amplification cascade machinery is developed for single-molecule detection of multiple repair glycosylases.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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