Near-infrared fluorescent dyes for enhanced contrast in optical mammography: phantom experiments

2001 ◽  
Vol 6 (2) ◽  
pp. 134 ◽  
Author(s):  
B. Ebert ◽  
U. Sukowski ◽  
D. Grosenick ◽  
H. Wabnitz ◽  
K. T. Moesta ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Dyes ◽  
Optical Mammography ◽  
Phantom Experiments

Estimation of detection limits of a clinical fluorescence optical mammography system for the near-infrared fluorophore IRDye800CW: phantom experiments

2012 ◽  
Vol 17 (7) ◽  
pp. 0760221 ◽  
Author(s):  
Arthur Adams ◽  
Jurgen E. M. Mourik ◽  
Marjolein van der Voort ◽  
Paul C. Pearlman ◽  
Tim Nielsen ◽  
...  
Keyword(s):  
Near Infrared ◽  
Detection Limits ◽  
Optical Mammography ◽  
Phantom Experiments

scholarly journals Synthesis of Silica Nanoparticles with Physical Encapsulation of Near-Infrared Fluorescent Dyes and Their Tannic Acid Coating

ACS Omega ◽  
2021 ◽  
Author(s):  
Yoshio Nakahara ◽  
Yukiho Nakajima ◽  
Soichiro Okada ◽  
Jun Miyazaki ◽  
Setsuko Yajima
Keyword(s):  
Silica Nanoparticles ◽  
Tannic Acid ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Acid Coating

scholarly journals INDOCYANINE GREEN ENHANCED LAPAROSCOPIC CHOLECYSTECTOMY IN ACUTE PATIENTS: DO WE NEED A REVISION OF THE SAFETY PARADIGMS?

2017 ◽  
Vol 64 (2) ◽  
pp. 151-154
Author(s):  
Catalin Alius ◽  
◽  
Eugen-Sebastian Gradinaru ◽  
Adriana Elena Nica ◽  
◽  
...  
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Augmented Reality ◽  
Surgical Technique ◽  
Indocyanine Green ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Operating Time ◽  
Biliary Anatomy ◽  
Early Laparoscopic Cholecystectomy ◽  
Small Cohort

Introduction. Rapid developments in medical technology have allowed the incorporation of Indocyanine green (ICG) fluorescent cholangyography in the surgical technique armamentarium. The visualization of the biliary anatomy with augmented reality devices during surgery reduces complications and offer the perspective of challenging the safety paradigms which prohibited surgery in certain acute biliary conditions. Materials and methods. 43 consecutive patients were enrolled in a prospective interventional study and randomly divided into a cohort of 19 patients who had ICG injected prior to laparoscopic cholecystectomy and a cohort of 23 patients who received no fluorescent dye prior to surgery. In the ICG lot a Near Infrared Fluorescent System was used for the acquisition of fluorescent data in order to produce real time augmented reality imaging (ICG fluorescent cholangiography). The surgical technique and the indications for surgery were the same for the same in both cohorts of patients. Results and discussion. The cohort of patients receiving ICG had no complications and the mean operating time was 10 minutes less. The biliary anatomy was identified immediately in the ICG cohort with a specificity of 89.4% for the common bule duct and 73.6% for the cystic duct. In the non ICG cohort 21% of the CBDs and 43.4% of the cystic ducts were identified with difficulty during the procedure. Conclusion. We demonstrated in a small cohort of patients that early laparoscopic cholecystectomy is safe and can be performed quicker with the aid of fluorescent dyes. In order to challenge the safety paradigms around the early laparoscopic cholecystectomy a larger study is necessary.

scholarly journals Optimization and functionalization of red-shifted rhodamine dyes

2019 ◽  
Author(s):  
Jonathan B. Grimm ◽  
Ariana N. Tkachuk ◽  
Heejun Choi ◽  
Boaz Mohar ◽  
Natalie Falco ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Poor Performance ◽  
Biological Imaging ◽  
Infrared Light ◽  
Long Wavelength ◽  
General Chemical ◽  
Chemical Groups ◽  
Rhodamine Dyes

ABSTRACTExpanding the palette of fluorescent dyes is vital for pushing the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.

scholarly journals The Use of Fluorescent Anti-CEA Antibodies to Label, Resect and Treat Cancers: A Review

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1819
Author(s):  
Michael A. Turner ◽  
Thinzar M. Lwin ◽  
Siamak Amirfakhri ◽  
Hiroto Nishino ◽  
Robert M. Hoffman ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Dyes ◽  
Review Article ◽  
In Vivo Studies ◽  
Murine Models ◽  
Exclusion Criteria ◽  
Major Barrier ◽  
Tumor Margins ◽  
Cancer Types

A major barrier to the diagnosis and effective treatment of solid-tumor cancers is the difficulty in detection and visualization of tumor margins in primary and metastatic disease. The use of fluorescence can augment the surgeon’s ability to detect cancer and aid in its resection. Several cancer types express carcinoembryonic antigen (CEA) including colorectal, pancreatic and gastric cancer. Antibodies to CEA have been developed and tagged with near-infrared fluorescent dyes. This review article surveyed the use of CEA antibodies conjugated to fluorescent probes for in vivo studies since 1990. PubMed and Google Scholar databases were queried, and 900 titles and abstracts were screened. Fifty-nine entries were identified as possibly meeting inclusion/exclusion criteria and were reviewed in full. Forty articles were included in the review and their citations were screened for additional entries. A total of 44 articles were included in the final review. The use of fluorescent anti-CEA antibodies has been shown to improve detection and resection of tumors in both murine models and clinically. The cumulative results indicate that fluorescent-conjugated anti-CEA antibodies have important potential to improve cancer diagnosis and surgery. In an emerging technology, anti-CEA fluorescent antibodies have also been successfully used for photoimmunotherapy treatment for cancer.

scholarly journals Optical Imaging of Kidney Cancer with Novel Near Infrared Heptamethine Carbocyanine Fluorescent Dyes

2013 ◽  
Vol 189 (2) ◽  
pp. 702-710 ◽  
Author(s):  
Xiaojian Yang ◽  
Chen Shao ◽  
Ruoxiang Wang ◽  
Chia-Yi Chu ◽  
Peizhen Hu ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Kidney Cancer ◽  
Near Infrared ◽  
Fluorescent Dyes

Abstract 4304: Evaluation of different near-infrared fluorescent dyes

2014 ◽  
Author(s):  
Maria P. Lambros ◽  
Antonio Sanchez ◽  
Surbhi Desai ◽  
Labanya Kondapalli
Keyword(s):  
Near Infrared ◽  
Fluorescent Dyes

Near-infrared small molecular fluorescent dyes for photothermal therapy

2019 ◽  
Vol 30 (7) ◽  
pp. 1353-1360 ◽  
Author(s):  
Yisha Chen ◽  
Li Li ◽  
Weijie Chen ◽  
Haiyan Chen ◽  
Jun Yin
Keyword(s):  
Photothermal Therapy ◽  
Near Infrared ◽  
Fluorescent Dyes

scholarly journals Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense OligonucleotidesIn Vivo

2014 ◽  
Vol 2014 ◽  
pp. 1-10
Author(s):  
Maria Chiara Munisso ◽  
Tetsuji Yamaoka
Keyword(s):  
Fluorescence Intensity ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Large Change ◽  
Optical Systems ◽  
Stable Complex ◽  
Tissue Samples ◽  
Reliable Assessment ◽  
New Methodologies

The availability of fluorescent dyes and the advances in the optical systems forin vivoimaging have stimulated an increasing interest in developing new methodologies to study and quantify the biodistribution of labeled agents. However, despite these great achievements, we are facing significant challenges in determining if the observed fluorescence does correspond to the quantity of the dye in the tissues. In fact, although the far-red and near-infrared lights can propagate through several centimetres of tissue, they diffuse within a few millimetres as consequence of the elastic scattering of photons. In addition, when dye-labeled oligonucleotides form stable complex with cationic carriers, a large change in the fluorescence intensity of the dye is observed. Therefore, the measured fluorescence intensity is altered by the tissue heterogeneity and by the fluctuation of dye intensity. Hence, in this study a quantification strategy for fluorescence-labeled oligonucleotides was developed to solve these disadvantageous effects. Our results proved that upon efficient homogenization and dilution with chaotropic agents, such as guanidinium thiocyanate, it is possible to achieve a complete fluorescence intensity recovery. Furthermore, we demonstrated that this method has the advantage of good sensitivity and reproducibility, as well as easy handling of the tissue samples.

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