scholarly journals Synthesis of Silica Nanoparticles with Physical Encapsulation of Near-Infrared Fluorescent Dyes and Their Tannic Acid Coating

ACS Omega ◽  
2021 ◽  
Author(s):  
Yoshio Nakahara ◽  
Yukiho Nakajima ◽  
Soichiro Okada ◽  
Jun Miyazaki ◽  
Setsuko Yajima
Keyword(s):  
Silica Nanoparticles ◽  
Tannic Acid ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Acid Coating

scholarly journals Shortwave Infrared Imaging with J-Aggregates Stabilized in Hollow Mesoporous Silica Nanoparticles

2018 ◽  
Author(s):  
Wei Chen ◽  
ChiAn Cheng ◽  
Emily Cosco ◽  
Shyam Ramakrishnan ◽  
Jakob Lingg ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Contrast Agents ◽  
Silica Nanoparticles ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
J Aggregates ◽  
Hollow Mesoporous Silica ◽  
Shortwave Infrared

Tissue is translucent to shortwave infrared (SWIR) light, rendering optical imaging superior in this region. However, the widespread use of optical SWIR imaging has been limited, in part, by the lack of bright, biocompatible contrast agents that absorb and emit light above 1000 nm. J-aggregation offers a means to transform stable, near-infrared (NIR) fluorophores into red-shifted SWIR contrast agents. Here we demonstrate that hollow mesoporous silica nanoparticles (HMSNs) can template the J-aggregation of NIR fluorophore IR-140 to result in nanomaterials that absorb and emit SWIR light. The J-aggregates inside PEGylated HMSNs are stable for multiple weeks in buffer and enable high resolution imaging <i>in vivo</i>with 980 nm excitation.

scholarly journals INDOCYANINE GREEN ENHANCED LAPAROSCOPIC CHOLECYSTECTOMY IN ACUTE PATIENTS: DO WE NEED A REVISION OF THE SAFETY PARADIGMS?

2017 ◽  
Vol 64 (2) ◽  
pp. 151-154
Author(s):  
Catalin Alius ◽  
◽  
Eugen-Sebastian Gradinaru ◽  
Adriana Elena Nica ◽  
◽  
...  
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Augmented Reality ◽  
Surgical Technique ◽  
Indocyanine Green ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Operating Time ◽  
Biliary Anatomy ◽  
Early Laparoscopic Cholecystectomy ◽  
Small Cohort

Introduction. Rapid developments in medical technology have allowed the incorporation of Indocyanine green (ICG) fluorescent cholangyography in the surgical technique armamentarium. The visualization of the biliary anatomy with augmented reality devices during surgery reduces complications and offer the perspective of challenging the safety paradigms which prohibited surgery in certain acute biliary conditions. Materials and methods. 43 consecutive patients were enrolled in a prospective interventional study and randomly divided into a cohort of 19 patients who had ICG injected prior to laparoscopic cholecystectomy and a cohort of 23 patients who received no fluorescent dye prior to surgery. In the ICG lot a Near Infrared Fluorescent System was used for the acquisition of fluorescent data in order to produce real time augmented reality imaging (ICG fluorescent cholangiography). The surgical technique and the indications for surgery were the same for the same in both cohorts of patients. Results and discussion. The cohort of patients receiving ICG had no complications and the mean operating time was 10 minutes less. The biliary anatomy was identified immediately in the ICG cohort with a specificity of 89.4% for the common bule duct and 73.6% for the cystic duct. In the non ICG cohort 21% of the CBDs and 43.4% of the cystic ducts were identified with difficulty during the procedure. Conclusion. We demonstrated in a small cohort of patients that early laparoscopic cholecystectomy is safe and can be performed quicker with the aid of fluorescent dyes. In order to challenge the safety paradigms around the early laparoscopic cholecystectomy a larger study is necessary.

scholarly journals Optimization and functionalization of red-shifted rhodamine dyes

2019 ◽  
Author(s):  
Jonathan B. Grimm ◽  
Ariana N. Tkachuk ◽  
Heejun Choi ◽  
Boaz Mohar ◽  
Natalie Falco ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Poor Performance ◽  
Biological Imaging ◽  
Infrared Light ◽  
Long Wavelength ◽  
General Chemical ◽  
Chemical Groups ◽  
Rhodamine Dyes

ABSTRACTExpanding the palette of fluorescent dyes is vital for pushing the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.

scholarly journals Bright and stable near-infrared Pluronic–silica nanoparticles as contrast agents for in vivo optical imaging

2016 ◽  
Vol 4 (33) ◽  
pp. 5560-5566 ◽  
Author(s):  
Lesan Yan ◽  
Huiquan Wang ◽  
Anqi Zhang ◽  
Calvin Zhao ◽  
Yongping Chen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Silica Nanoparticles ◽  
Near Infrared ◽  
Circulation Time ◽  
In Vivo Optical Imaging

The IR780@NPs exhibited excellent characteristics for in vivo imaging with a long circulation time and high retention in tumor and sentinel lymph node.

scholarly journals The Use of Fluorescent Anti-CEA Antibodies to Label, Resect and Treat Cancers: A Review

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1819
Author(s):  
Michael A. Turner ◽  
Thinzar M. Lwin ◽  
Siamak Amirfakhri ◽  
Hiroto Nishino ◽  
Robert M. Hoffman ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Dyes ◽  
Review Article ◽  
In Vivo Studies ◽  
Murine Models ◽  
Exclusion Criteria ◽  
Major Barrier ◽  
Tumor Margins ◽  
Cancer Types

A major barrier to the diagnosis and effective treatment of solid-tumor cancers is the difficulty in detection and visualization of tumor margins in primary and metastatic disease. The use of fluorescence can augment the surgeon’s ability to detect cancer and aid in its resection. Several cancer types express carcinoembryonic antigen (CEA) including colorectal, pancreatic and gastric cancer. Antibodies to CEA have been developed and tagged with near-infrared fluorescent dyes. This review article surveyed the use of CEA antibodies conjugated to fluorescent probes for in vivo studies since 1990. PubMed and Google Scholar databases were queried, and 900 titles and abstracts were screened. Fifty-nine entries were identified as possibly meeting inclusion/exclusion criteria and were reviewed in full. Forty articles were included in the review and their citations were screened for additional entries. A total of 44 articles were included in the final review. The use of fluorescent anti-CEA antibodies has been shown to improve detection and resection of tumors in both murine models and clinically. The cumulative results indicate that fluorescent-conjugated anti-CEA antibodies have important potential to improve cancer diagnosis and surgery. In an emerging technology, anti-CEA fluorescent antibodies have also been successfully used for photoimmunotherapy treatment for cancer.

Role of Aqueous-Phase Calcination in Synthesis of Ultra-Stable Dye-Embedded Fluorescent Nanoparticles for Cellular Probing

2021 ◽  
pp. 000370282110275
Author(s):  
Rachel Rex ◽  
Soumik Siddhanta ◽  
Ishan Barman
Keyword(s):  
Silica Nanoparticles ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Aqueous Media ◽  
Clinical Diagnostics ◽  
Live Cell ◽  
Metal Nanoparticle ◽  
Superior Performance ◽  
Fluorescent Nanoparticles

Fluorescence imaging is a major driver of discovery in biology, and an invaluable asset in clinical diagnostics. To overcome quenching limitations of conventional fluorescent dyes and further improve intensity, nanoparticle-based constructs have been the subject of intense investigation, and within this realm, dye-doped silica-coated nanoparticles have garnered significant attention. Despite their growing popularity in research, fluorescent silica nanoparticles suffer from a significant flaw. The degradation of these nanoparticles in biological media by hydrolytic dissolution is underreported, leading to serious misinterpretations, and limiting their applicability for live cell and in vivo imaging. Here, the development of an ultra-stable, dye-embedded, silica-coated metal nanoparticle is reported, and its superior performance in long-term live cell imaging is demonstrated. While conventional dye-doped silica nanoparticles begin to degrade within an hour in aqueous media, by leveraging a modified liquid calcination process, this new construct is shown to be stable for at least 24 h. The stability of this metal-enhanced fluorescent probe in biologically relevant temperatures and media, and its demonstrated utility for cell imaging, paves the way for its future adoption in biomedical research.

scholarly journals Bioinspired programmable wettability arrays for droplets manipulation

2020 ◽  
Vol 117 (9) ◽  
pp. 4527-4532 ◽  
Author(s):  
Lingyu Sun ◽  
Feika Bian ◽  
Yu Wang ◽  
Yuetong Wang ◽  
Xiaoxuan Zhang ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Near Infrared ◽  
Hydrophobic Surface ◽  
Transformation Property ◽  
Laboratory Research ◽  
Liquid Droplets ◽  
Energy Transformation ◽  
Droplet Transfer ◽  
Hydrophobic Property ◽  
Trimethylolpropane Triacrylate

The manipulation of liquid droplets demonstrates great importance in various areas from laboratory research to our daily life. Here, inspired by the unique microstructure of plant stomata, we present a surface with programmable wettability arrays for droplets manipulation. The substrate film of this surface is constructed by using a coaxial capillary microfluidics to emulsify and pack graphene oxide (GO) hybridN-isopropylacrylamide (NIPAM) hydrogel solution into silica nanoparticles-dispersed ethoxylated trimethylolpropane triacrylate (ETPTA) phase. Because of the distribution of the silica nanoparticles on the ETPTA interface, the outer surface of the film could achieve favorable hydrophobic property under selective fluorosilane decoration. Owing to the outstanding photothermal energy transformation property of the GO, the encapsulated hydrophilic hydrogel arrays could shrink back into the holes to expose their hydrophobic surface with near-infrared (NIR) irradiation; this imparts the composite film with remotely switchable surface droplet adhesion status. Based on this phenomenon, we have demonstrated controllable droplet sliding on programmable wettability pathways, together with effective droplet transfer for printing with mask integration, which remains difficult to realize by existing techniques.

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