scholarly journals Nanoscaffold's stiffness affects primary cortical cell network formation

2014 ◽  
Vol 32 (6) ◽  
pp. 06FD03 ◽  
Author(s):  
Sijia Xie ◽  
Bart Schurink ◽  
Floor Wolbers ◽  
Regina Luttge ◽  
Gerco Hassink
Keyword(s):  
Network Formation ◽  
Cortical Cell ◽  
Cell Network ◽  
Primary Cortical Cell

scholarly journals Acoustic Cell Patterning in Hydrogel for Three-Dimensional Cell Network Formation

Micromachines ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
Kyo-in Koo ◽  
Andreas Lenshof ◽  
Le Thi Huong ◽  
Thomas Laurell
Keyword(s):  
Network Formation ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Extrusion Process ◽  
Fibroblast Cells ◽  
Glass Capillary ◽  
Cell Network ◽  
Significant Difference ◽  
The One ◽  
Umbilical Vein Endothelial Cells

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.

scholarly journals Less is More: Oligomer extraction and hydrothermal annealing increase PDMS bonding forces for new microfluidics assembly and for biological studies

2017 ◽  
Author(s):  
L. J. Millet ◽  
A. Jain ◽  
M. U. Gillette
Keyword(s):  
Network Formation ◽  
Low Cost ◽  
Covalent Bond ◽  
High Strength ◽  
Combined Processes ◽  
Less Is More ◽  
Cell Network ◽  
Biological Studies ◽  
Clinical Biology ◽  
Network Studies

Key determinants in the emergence of complex cellular morphologies and functions are cues in the micro-environment. Primary among these is the presence of neighboring cells as networks form. Therefore, for high-resolution analysis, it is crucial to develop micro-environments that permit exquisite control of network formation. This is especially true in cell science, tissue engineering, and clinical biology. We introduce a new approach for assembling polydimethylsiloxane (PDMS)-based microfluidic environments that enhances cell network formation and analyses. We report that the combined processes of PDMS solvent-extraction (E-PDMS) and hydrothermal annealing create unique conditions that produce high-strength bonds between E-PDMS and glass – properties not associated with conventional PDMS. Extraction followed by hydrothermal annealing removes unbound oligomers, promotes polymer cross-linking, facilitates covalent bond formation with glass, and retains the highest biocompatibility. Our extraction protocol accelerates oligomer removal from 5 to 2 days. Resulting microfluidic platforms are uniquely suited for cell-network studies owing to high bond strengths, effectively corralling cellular extensions and eliminating harmful oligomers. We demonstrate simple, simultaneous actuation of multiple microfluidic domains for invoking ATP- and glutamate-induced Ca2+ signaling in glial-cell networks. These low-cost, simple E-PMDS modifications and flow manipulations further enable microfluidic technologies for cell-signaling and network studies as well as novel applications.

Mechanical influences on endothelial cell network formation in vitro

2010 ◽  
Vol 19 (03) ◽  
pp. 250-254 ◽  
Author(s):  
G. Kasper ◽  
K. M. van Scherpenzeel ◽  
P. Simon ◽  
H. J. Bail ◽  
M. A. Schütz ◽  
...  
Keyword(s):  
Mechanical Loading ◽  
Network Formation ◽  
Biochemical Modulation ◽  
Cell Network ◽  
Cyclic Stretching ◽  
Cyclic Compression ◽  
Responsible Mechanism ◽  
Tgf Β1 ◽  
Stimulation Of

SummaryWhile both the restoration of the blood supply and an appropriate local mechanical environment are critical for uneventful bone healing, their influence on each other remains unclear. Human bone fracture haematomas (< 72 h post-trauma) were cultivated for 3 days in fibrin matrices, with or without cyclic compression. Conditioned medium from these cultures enhanced the formation of vessel-like networks by HMEC-1 cells, and mechanical loading further elevated it, without affecting the cells’ metabolic activity. While haematomas released the angiogenesis-regulators, VEGF and TGF-β1, their concentrations were not affected by mechanical loading. However, direct cyclic stretching of the HMEC-1 cells decreased network formation. The appearance of the networks and a trend towards elevated VEGF under strain suggested physical disruption rather than biochemical modulation as the responsible mechanism. Thus, early fracture haematomas and their mechanical loading increase the paracrine stimulation of endothelial organisation in vitro, but direct periodic strains may disrupt or impair vessel assembly in otherwise favourable conditions.

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