Application of EBL fabricated nanostructured substrates for surface enhanced Raman spectroscopy detection of protein A in aqueous solution

2013 ◽  
Vol 31 (6) ◽  
pp. 06F901 ◽  
Author(s):  
Luis Gutierrez-Rivera ◽  
Robert F. Peters ◽  
Steven K. Dew ◽  
Maria Stepanova
Keyword(s):  
Aqueous Solution ◽  
Raman Spectroscopy ◽  
Protein A ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Nanostructured Substrates

A femtogram level competitive immunoassay of mercury(ii) based on surface-enhanced Raman spectroscopy

2014 ◽  
Vol 50 (65) ◽  
pp. 9112-9114 ◽  
Author(s):  
Yuzhen Wang ◽  
Shuai Chen ◽  
Chao Wei ◽  
Minmin Xu ◽  
Jianlin Yao ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Raman Spectroscopy ◽  
Specific Surface ◽  
Surface Enhanced Raman Spectroscopy ◽  
Competitive Immunoassay ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A femtogram level and specific surface enhanced Raman spectroscopy (SERS) based competitive immunoassay was developed to detect Hg(ii) in aqueous solution.

scholarly journals Surface-Enhanced Raman Spectroscopy of Carbon Nanotubes in Aqueous Solution

2012 ◽  
Vol 70 (14) ◽  
pp. 1533 ◽  
Author(s):  
Yang Niu ◽  
Qinghai Liu ◽  
Juan Yang ◽  
Dongliang Gao ◽  
Xiaojun Qin ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Aqueous Solution ◽  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Detection of resistance protein A (MxA) in paper-based immunoassays with surface enhanced Raman spectroscopy with AuAg nanoshells

Nanoscale ◽  
2019 ◽  
Vol 11 (22) ◽  
pp. 10819-10827 ◽  
Author(s):  
Lorenzo Russo ◽  
Maria Sánchez-Purrà ◽  
Cristina Rodriguez-Quijada ◽  
Brianna M. Leonardo ◽  
Victor Puntes ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Protein A ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Resistance Protein

Hollow AuAg nanoshells enable Surface Enhanced Raman Spectroscopy readout of a paper immunoassay for myxovirus protein A (MxA), a biomarker that can distinguish viral vs. bacterial infections.

Droplet based microfluidics: spectroscopic characterization of levofloxacin and its SERS detection

2015 ◽  
Vol 17 (33) ◽  
pp. 21236-21242 ◽  
Author(s):  
I. J. Hidi ◽  
M. Jahn ◽  
K. Weber ◽  
D. Cialla-May ◽  
J. Popp
Keyword(s):  
Aqueous Solution ◽  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Spectroscopic Characterization ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection

The determination of the absorption behavior of levofloxacin (levaquin) on the surface of silver nanoparticles and its determination in aqueous solution by droplet based microfluidics combined with surface enhanced Raman spectroscopy.

scholarly journals SARS-CoV-2 Receptor Binding Domain as a Stable-Potential Target for SARS-CoV-2 Detection by Surface—Enhanced Raman Spectroscopy

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4617
Author(s):  
Chawki Awada ◽  
Mohammed Mahfoudh BA Abdullah ◽  
Hassan Traboulsi ◽  
Chahinez Dab ◽  
Adil Alshoaibi
Keyword(s):  
Raman Spectroscopy ◽  
Detection Limit ◽  
Protein A ◽  
Protein Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Perfect Agreement ◽  
Antibody Recognition ◽  
Surface Enhanced ◽  
Single Protein ◽  
Surface Enhanced Raman

In this work, we report a new approach for detecting SARS-CoV-2 RBD protein (RBD) using the surface-enhanced Raman spectroscopy (SERS) technique. The optical enhancement was obtained thanks to the preparation of nanostructured Ag/Au substrates. Fabricated Au/Ag nanostructures were used in the SERS experiment for RBD protein detection. SERS substrates show higher capabilities and sensitivity to detect RBD protein in a short time (3 s) and with very low power. We were able to push the detection limit of proteins to a single protein detection level of 1 pM. The latter is equivalent to 1 fM as a detection limit of viruses. Additionally, we have shown that the SERS technique was useful to figure out the presence of RBD protein on antibody functionalized substrates. In this case, the SERS detection was based on protein-antibody recognition, which led to shifts in the Raman peaks and allowed signal discrimination between RBD and other targets such as Bovine serum albumin (BSA) protein. A perfect agreement between a 3D simulated model based on finite element method and experiment was reported confirming the SERS frequency shift potential for trace proteins detection. Our results could open the way to develop a new prototype based on SERS sensitivity and selectivity for rapid detection at a very low concentration of virus and even at a single protein level.

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