scholarly journals The Fate of Beta-Lactam Resistance Determinats within the Wastewater Treatment Plant in Żywiec and in Final Effluent Receiving Żywieckie Lake

Author(s):  
Kinga Bondarczuk ◽  
Zofia Piotrowska-Seget
2021 ◽  
Author(s):  
Miguel Uyaguari

Abstract Background: Wastewater treatment plants are an essential part of maintaining the health and safety of the general public. However, they are also an anthropogenic source of antibiotic resistance genes. In this study, we characterized the resistome, the distribution of classes 1-3 integron-integrase genes (intI1, intI2, and intI3) as mobile genetic element biomarkers, and the bacterial and phage community compositions in the North End Sewage Treatment Plant in Winnipeg, Manitoba. Samples were collected from raw sewage, returned activated sludge, final effluent, and dewatered sludge. A total of 28 bacterial and viral metagenomes were sequenced over two seasons, fall and winter. Integron-integrase genes, the 16S rRNA gene, and the coliform beta-glucuronidase gene were also quantified during this time period. Results: Bacterial classes observed above 1% relative abundance in all treatments were Actinobacteria (39.24% ± 0.25%), Beta-proteobacteria (23.99% ± 0.16%), Gamma-proteobacteria (11.06% ± 0.09%), and Alpha-proteobacteria (9.18 ± 0.04%). Families within the Caudovirales order: Siphoviridae (48.69% ± 0.10%), Podoviridae (23.99% ± 0.07%), and Myoviridae (19.94% ± 0.09%) were the dominant phage observed throughout the NESTP. The most abundant bacterial genera (in terms of average percent relative abundance) in influent, returned activated sludge, final effluent, and sludge, respectively, includes Mycobacterium (37.4%, 18.3%, 46.1%, and 7.7%), Acidovorax (8.9%, 10.8%, 5.4%, and 1.3%), and Polaromonas (2.5%, 3.3%, 1.4%, and 0.4%).The most abundant class of antibiotic resistance in bacterial samples was tetracycline resistance (17.86% ± 0.03%) followed by peptide antibiotics (14.24% ± 0.03%), and macrolides (10.63% ± 0.02%). Similarly, the phage samples contained a higher prevalence of macrolide (30.12% ± 0.30%), peptide antibiotic (10.78% ± 0.13%), and tetracycline (8.69% ± 0.11%) resistance. In addition, intI1 was the most abundant integron-integrase gene throughout treatment (1.14x104 gene copies/mL) followed by intI3 (4.97x103 gene copies/mL) while intI2 abundance remained low (6.4x101 gene copies/mL).Conclusions: The wastewater treatment plant successfully reduced the abundance of bacteria, DNA bacteriophages, and antibiotic resistance genes although many of them still remained in effluent and biosolids. The presence of integron-integrase genes throughout treatment and in effluent suggests that antibiotic resistance genes could be actively disseminating resistance between both environmental and pathogenic bacteria.


2021 ◽  
Vol 8 ◽  
Author(s):  
Lisa M. Erdle ◽  
Dorsa Nouri Parto ◽  
David Sweetnam ◽  
Chelsea M. Rochman

Washing clothing is a known pathway for microfibers to reach the environment. Previous research has investigated microfiber capture close to the source (i.e., the washing machine), and demonstrated washing machine filters as a potential mitigation strategy. Widespread deployment into homes may be an effective solution to prevent microfiber emissions. Here, we investigated the effectiveness of washing machine filters at the level of a community. We installed filters in 97 homes in a small town, representing approximately 10% of households connected to the municipal wastewater treatment plant (WWTP). We evaluated treated final effluent and found a significant reduction in microfibers after filter installation. Furthermore, lint samples from filters revealed an average weekly lint capture of 6.4 g, equivalent to 179,200–2,707,200 microfibers. This research shows that microfiber filters on washing machines are effective at scale, and this result can help inform policy decisions to reduce microfiber emissions from laundering textiles.


2006 ◽  
Vol 54 (11-12) ◽  
pp. 301-308 ◽  
Author(s):  
E. Haramoto ◽  
H. Katayama ◽  
K. Oguma ◽  
H. Yamashita ◽  
A. Tajima ◽  
...  

The seasonal profiles of microorganisms in raw sewage, secondary-treated sewage, and final effluent at a wastewater treatment plant in Tokyo, Japan, were quantitatively determined each month for one year, from July 2003 to June 2004. Human noroviruses, which were determined by real-time PCR, in raw sewage varied from 0.17–260 copies/mL for genotype 1 and from 2.4–1900 copies/mL for genotype 2, showing much higher values in winter, the epidemic season. The concentration of total coliforms, Escherichia coli, or F-specific phages in raw sewage was almost constant throughout the year. Human noroviruses of genotype 2 were removed most effectively (3.69 log10 on average) at the wastewater treatment plant, followed by E. coli (3.37 log10), total coliforms (3.05 log10), F-specific phages (2.81 log10), and human noroviruses of genotype 1 (2.27 log10). The removal ratio of human noroviruses was almost constant, independent of the initial concentration of the viruses in raw sewage, which led to the increasing concentration of human noroviruses in final effluent in winter. None of the tested bacteria was judged to be a reliable indicator of human noroviruses in final effluent.


2014 ◽  
Vol 69 (11) ◽  
pp. 2221-2229 ◽  
Author(s):  
M. Kim ◽  
P. Guerra ◽  
A. Shah ◽  
M. Parsa ◽  
M. Alaee ◽  
...  

Ninety-nine pharmaceuticals and personal care products (PPCPs) were analyzed in influent, final effluent, and biosolids samples from a wastewater treatment plant employing a membrane bioreactor (MBR). High concentrations in influent were found for acetaminophen, caffeine, metformin, 2-hydroxy-ibuprofen, paraxanthine, ibuprofen, and naproxen (104–105 ng/L). Final effluents contained clarithromycin, metformin, atenolol, carbamazepine, and trimethoprim (>500 ng/L) at the highest concentrations, while triclosan, ciprofloxacin, norfloxacin, triclocarban, metformin, caffeine, ofloxacin, and paraxanthine were found at high concentrations in biosolids (>103 ng/g dry weight). PPCP removals varied from −34% to >99% and 23 PPCPs had ≥90% removal. Of the studied PPCPs, 26 compounds have been rarely or never studied in previous membrane bioreactor (MBR) investigations. The removal pathway showed that acetaminophen, 2-hydroxy-ibuprofen, naproxen, ibuprofen, codeine, metformin, enalapril, atorvastatin, caffeine, paraxanthine, and cotinine exhibited high degradation/transformation. PPCPs showing strong sorption to solids included triclocarban, triclosan, miconazole, tetracycline, 4-epitetracycline, norfloxacin, ciprofloxacin, doxycycline, paroxetine, and ofloxacin. Trimethoprim, oxycodone, clarithromycin, thiabendazole, hydrochlorothiazide, erythromycin-H2O, carbamazepine, meprobamate, and propranolol were not removed during treatment, and clarithromycin was even formed during treatment. This investigation extended our understanding of the occurrence and fate of PPCPs in an MBR process through the analysis of the largest number of compounds in an MBR study to date.


2009 ◽  
Vol 59 (5) ◽  
pp. 1019-1024 ◽  
Author(s):  
Cao Guomin ◽  
Yang Guoping ◽  
Sheng Mei ◽  
Wang Yongjian

Wastewaters from phenol and rubber synthesis were treated by the activated sludge process in a large-scale chemical factory in Shanghai, but the final effluent quality cannot conform with the local discharge limit without using river water for dilution. Therefore, this chemical factory had to upgrade its wastewater treatment plant. To fully use the present buildings and equipment during upgrading of the chemical factory's wastewater treatment plant and to save operation costs, a sequential biological pre-treatement, chemical oxidation, and biological post-treatment (or BCB for short) process had been proposed and investigated in a pilot trial. The pilot trial results showed that about 80% COD in the chemical wastewater could be removed through anoxic and aerobic degradation in the biological pre-treatement section, and the residual COD in the effluent of the biological pre-treatment section belongs to refractory chemicals which cannot be removed by the normal biological process. The refractory chemicals were partial oxidized using Fenton's reagent in the chemical oxidation section to improve their biodegradability; subsequently the wastewater was treated by the SBR process in the biological post-treatment section. The final effluent COD reached the first grade discharge limit (<100 mg l−1) of Chinese Notational Integrated Wastewater Discharge Standard (GB8978-1996) even if without using any dilution water. Compared with the original dilution and biological process, the operation cost of the BCB process increased by about 0.5 yuan (RMB) per cubic metre wastewater, but about 1,240,000 m3 a−1 dilution water could be saved and the COD emission could be cut down by 112 tonne each year.


2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.


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