Morphological ontogeny of Damaeolus ornatissimus (Acari: Oribatida: Damaeolidae), with comments on Damaeolus Paoli

2020 ◽  
Vol 25 (3) ◽  
pp. 459-478
Author(s):  
Stanisław Seniczak ◽  
Otilia Ivan ◽  
Anna Seniczak

The morphological ontogeny of Damaeolus ornatissimus Csiszár, 1962 from Romania is described and illustrated. The nymphs of this species are quadrideficient and eupheredermous, i.e. they carry the exuvial scalps of previous instars on the gastronotum. The bothridial seta of juveniles is fusiform, with long apical flagellum, and all prodorsal and gastronotal setae are smooth, and some of them are covered with thick layer of granular cerotegument. The larva has polygonal pattern on the gastronotum, two pairs of setae on paraproctal valves and 12 pairs of gastronotal setae, the nymphs have 12 pairs of latter setae. In all instars, seta d on all genua and tibiae is present.

Author(s):  
M.D. Bentzon ◽  
J. v. Wonterghem ◽  
A. Thölén

We report on the oxidation of a magnetic fluid. The oxidation results in magnetic super lattice crystals. The “atoms” are hematite (α-Fe2O3) particles with a diameter ø = 6.9 nm and they are covered with a 1-2 nm thick layer of surfactant molecules.Magnetic fluids are homogeneous suspensions of small magnetic particles in a carrier liquid. To prevent agglomeration, the particles are coated with surfactant molecules. The magnetic fluid studied in this work was produced by thermal decomposition of Fe(CO)5 in Declin (carrier liquid) in the presence of oleic acid (surfactant). The magnetic particles consist of an amorphous iron-carbon alloy. For TEM investigation a droplet of the fluid was added to benzine and a carbon film on a copper net was immersed. When exposed to air the sample starts burning. The oxidation and electron irradiation transform the magnetic particles into hematite (α-Fe2O3) particles with a median diameter ø = 6.9 nm.


Author(s):  
G.Y. Fan ◽  
Bruce Mrosko ◽  
Mark H. Ellisman

A lens coupled CCD camera showing single electron sensitivity has been built for TEM applications. The design is illustrated in Fig. 1. The bottom flange of a JEM-4000EX microscope is replaced by a special flange which carries a large rectangular leaded glass window, 22 mm thick. A 20 μm thick layer of red phosphor is coated on the window, and the entire window is sputter-coated with a thin layer of Au/Pt. A two-lens relay system is used to provide efficient coupling between the image on the phosphor scintillator and the CCD imager. An f1.0 lens (Goerz optical) with front focal length 71.6 mm is used as the collector. A mirror prism, of the Amici type, is used to "bend" the optical path by 90° to prevent X-rays which may penetrate the leaded glass from hitting the CCD detector. Images may be relayed directly to the camera (1:1) or demagnified by a factor of up to 3:1 by moving the lens assembly.


Author(s):  
Randy Moore

Previous work has indicated that the graft incompatihility between Sedrmi telephoides and Solanum pennellil involves cell necrosis that results In a thick layer of collapsed cells at the graft Interface. This necrotic layer insulates the stock from the scion, which results in abscission of the Sedum scion after 4-6 weeks due to desiccation and starvation. Thus, cell autolysis (which is restricted to Sedum) characterizes the Incompatibility response in this system (1). In order to elucidate the events that lead to cell autolysis, and thus better understand the cellular site and mode of action of cellular incompatibility, the appearance and fate of the hydrolytlc enzyme acid phosphatase (AP) was followed in both the compatible Sedum autograft and the incompatible Sedum/Solanum heterograft. Acid phosphatase was localized by a modified Gomori-type reaction; positive (i.e., including NaF inhibitor) and negative (lacking substrate) controls showed no enzymatic precipitate. Following an initial association with the endoplasmic reticulum (ER) and dictyosomes at 6-10 hours after grafting, AP activity in the compatible Sedum autograft is associated primarily with the plasmalemma (Fig. 1). By 18-24 hours after grafting, the AP activity is restricted to the tono-plast and vacuole (Fig. 2). This strict compartmentation and absence of enzyme from the cytosol is maintained throughout the development of the compatible graft. While AP activity in the incompatible Sedum/Solanum heterograft is Initially similar to the compatible Sedum autograft (i.e., initially found on the ER and dictyosomes), there is a marked difference in enzyme localization in the two graft partners as the incompatibility response develops. As in the compatible autograft, Solanum cells at the graft interface show an Increase in AP activity that Is restricted to the vacuole and tonoplast, with little or no enzyme activity in the cytosol (Fig. 3). In comparable Sedum cells, however, there is a dramatic Increase In AP activity in the cytosol (Fig. h); this cytosollc AP activity is associated with thin fibril-like structures (Fig. 5) measuring approximately 60 A in diameter. This high cytoplasmic AP activity In Sedum cells results in cell autolysis, death, and eventual cell collapse to form the characteristic necrotic layer separating the two graft partners.


2020 ◽  
Vol 89 (3) ◽  
pp. 30201 ◽  
Author(s):  
Xi Guan ◽  
Shiyu Wang ◽  
Wenxing Liu ◽  
Dashan Qin ◽  
Dayan Ban

Organic solar cells based on planar copper phthalocyanine (CuPc)/C60 heterojunction have been characterized, in which a 2 nm-thick layer of bathocuproine (BCP) is inserted into the CuPc layer. The thin layer of BCP allows hole current to tunnel it through but blocks the exciton diffusion, thereby altering the steady-state exciton profile in the CuPc zone (zone 1) sandwiched between BCP and C60. The short-circuit current density (JSC) of device is limited by the hole-exciton scattering effect at the BCP/CuPc (zone 1) interface. Based on the variation of JSC with the width of zone 1, the exciton diffusion length of CuPc is deduced to be 12.5–15 nm. The current research provides an easy and helpful method to determine the exciton diffusion lengths of organic electron donors.


Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 971
Author(s):  
Young Soo Yu ◽  
Chi Bum Ahn ◽  
Kuk Hui Son ◽  
Jin Woo Lee

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.


Materials ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 143 ◽  
Author(s):  
Alesia Paddubskaya ◽  
Marina Demidenko ◽  
Konstantin Batrakov ◽  
Gintaras Valušis ◽  
Tommi Kaplas ◽  
...  

By exploring the Salisbury screen approach, we propose and demonstrate a thin film absorber of terahertz (THz) radiation. The absorber is comprised of a less than 100 nm thick layer of pyrolytic carbon deposited on a stretchable polydimethylsiloxane (PDMS) film followed by the metal film. We demonstrate that being overall less than 200 microns thick, such a sandwich structure absorbs resonantly up to 99.9%of the incident THz radiation, and that the absorption resonance is determined by the polymer thickness, which can be adjusted by stretching.


Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 379
Author(s):  
Domagoj Mudronja ◽  
Frederik Vanmeert ◽  
Stjepko Fazinic ◽  
Koen Janssens ◽  
Darko Tibljas ◽  
...  

Stone monuments and buildings are susceptible to weathering. Carbonate-based stones are especially vulnerable in acidic environments, whereas magmatic acidic stones are more susceptible to chemical weathering in basic environments. To slow down surface corrosion of limestone and marble artworks/buildings, protective coatings which inhibit calcite dissolution have been proposed. In this work, samples from two stone types with different porosity were treated with ammonium oxalate (AmOx) to create a protective layer of calcium oxalate (CaOx) using the previously developed brushing method. Two different synchrotron microscopy experiments were performed to determine its protective capability. X-ray powder diffraction (SR-μ-XRPD) in transmission geometry allowed visualization of the distributions of calcium carbonate and oxalates along the sample depths. In a second step, X-ray fluorescence (SR-μ-XRF) was used to check the efficiency/integrity of the protective surface coating layer. This was done by measuring the sulfur distribution on the stone surface after exposing the protected stones to sulfuric acid. XRPD showed the formation of a protective oxalate layer with a thickness of 5–15 µm on the less porous stone, while a 20–30 µm thick layer formed on the more porous stone. The XRF study showed that the optimal treatment time depends on the stone porosity. Increasing the treatment time from 1 to 3 h resulted in a decreased efficiency of the protective layer for the low porosity stone. We assume that this is due to the formation of vertical channels (cracks) in the protective layer.


2019 ◽  
Vol 23 (3) ◽  
pp. 1611-1631 ◽  
Author(s):  
Ilari Lehtonen ◽  
Ari Venäläinen ◽  
Matti Kämäräinen ◽  
Antti Asikainen ◽  
Juha Laitila ◽  
...  

Abstract. Trafficability in forest terrain is controlled by ground-bearing capacity, which is crucial from the timber harvesting point of view. In winter, soil frost affects the most the bearing capacity, especially on peatland soils which have in general low bearing capacity. Ground frost similarly affects the bearing capacity of forest truck roads. A 20 cm thick layer of frozen soil or 40 cm thick layer of snow on the ground may already be sufficient for heavy forest harvesters. In this work, we studied the impacts of climate change on soil frost conditions and, consequently, on ground-bearing capacity from the timber harvesting point of view. The number of days with good wintertime bearing capacity was modelled by using a soil temperature model with a snow accumulation model and wide set of downscaled climate model data until the end of the 21st century. The model was calibrated for different forest and soil types. The results show that by the mid-21st century, the conditions with good bearing capacity will decrease in wintertime in Finland, most likely by about 1 month. The decrease in soil frost and wintertime bearing capacity will be more pronounced during the latter half of the century, when drained peatlands may virtually lack soil frost in most of winters in southern and western Finland. The projected decrease in the bearing capacity, accompanied with increasing demand for wood harvesting from drained peatlands, induces a clear need for the development of sustainable and resource-efficient logging practices for drained peatlands. This is also needed to avoid unnecessary harvesting damages, like rut formation on soils and damage to tree roots and stems.


Author(s):  
Hamid Ait Abderrahamane ◽  
Kamran Siddiqui ◽  
Georgios Vatistas

This paper deals with the dynamics of polygonal shapes resulting from the symmetry breaking of hollow-vortex core in a shallow water layer produced by a rotating disk near the bottom within a stationary cylindrical container. These polygonal shapes are investigated through image analysis. It is found that a given polygon rotates at the frequency close to one–third of the corresponding disk frequency and the flow dynamics around the apexes of the polygon is characterized by a frequency which is close to one–third of the frequency of the given polygonal pattern. The results also suggest a possible resonance between the satellite vortices at the apexes of the patterns and the bulk flow.


2015 ◽  
Vol 2015 ◽  
pp. 1-18 ◽  
Author(s):  
Alex Shlemov ◽  
Nina Golyandina ◽  
David Holloway ◽  
Alexander Spirov

Recent progress in microscopy technologies, biological markers, and automated processing methods is making possible the development of gene expression atlases at cellular-level resolution over whole embryos. Raw data on gene expression is usually very noisy. This noise comes from both experimental (technical/methodological) and true biological sources (from stochastic biochemical processes). In addition, the cells or nuclei being imaged are irregularly arranged in 3D space. This makes the processing, extraction, and study of expression signals and intrinsic biological noise a serious challenge for 3D data, requiring new computational approaches. Here, we present a new approach for studying gene expression in nuclei located in a thick layer around a spherical surface. The method includes depth equalization on the sphere, flattening, interpolation to a regular grid, pattern extraction by Shaped 3D singular spectrum analysis (SSA), and interpolation back to original nuclear positions. The approach is demonstrated on several examples of gene expression in the zebrafish egg (a model system in vertebrate development). The method is tested on several different data geometries (e.g., nuclear positions) and different forms of gene expression patterns. Fully 3D datasets for developmental gene expression are becoming increasingly available; we discuss the prospects of applying 3D-SSA to data processing and analysis in this growing field.


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