Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes

2020 ◽  
Vol 142 (11) ◽  
Author(s):  
Elizabeth Feeney ◽  
Devis Galesso ◽  
Cynthia Secchieri ◽  
Francesca Oliviero ◽  
Roberta Ramonda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Inflammatory Arthritis ◽  
Tribological Behavior ◽  
Friction Coefficients ◽  
Low Concentrations ◽  
Synovial Fluids ◽  
Erratic Behavior ◽  
Lubricating Properties

Abstract Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients. Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype. The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA). The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement. Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities. In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA. While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients. Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin. Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior. Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.

The role of protein content on the steady and oscillatory shear rheology of model synovial fluids

Soft Matter ◽  
2014 ◽  
Vol 10 (32) ◽  
pp. 5965-5973 ◽  
Author(s):  
Z. Zhang ◽  
S. Barman ◽  
G. F. Christopher
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Acid Solution ◽  
Protein Content ◽  
Shear Viscosity ◽  
Steady Shear ◽  
Interfacial Rheology ◽  
Steady Shear Viscosity ◽  
Synovial Fluids

Model synovial fluid steady shear viscosity to hyaluronic acid solution are identical when interfacial rheology effects are removed.

Endothelin-1 Production by Human Synoviocytes

1998 ◽  
Vol 35 (2) ◽  
pp. 290-294 ◽  
Author(s):  
Hiroshi Yoshida ◽  
Yuji Imafuku ◽  
Morihiro Ohhara ◽  
Masayuki Miyata ◽  
Reji Kasukawa ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Inflammatory Arthritis ◽  
Synovial Cells ◽  
Type B ◽  
Serum Samples ◽  
Synovial Macrophage ◽  
Significant Difference ◽  
Synovial Fluids ◽  
Synovial Tissues

Immunoreactive (ir)-endothelin (ET)-l concentrations in serum samples and synovial fluids from patients with rheumatoid arthritis were higher than concentrations in sera obtained from healthy volunteers. No significant difference in ir-ET-1 concentrations in synovial fluid was observed between rheumatoid arthritis patients and osteoarthritis patients. Cultured fluids of synovial cells collected from synovial tissues and leucocytes from synovial fluids of rheumatoid arthritis patients were studied to determine the origin of ir-ET-1 in synovial fluids. Ir-ET-1 was detected in the cultured fluids of synovial macrophage-like type A cells, but not in those of fibroblast-like type B cells from the synovial tissues or leucocytes from the synovial fluids. Longitudinal studies showed that the ir-ET-1 concentration in the cultured fluid reached a peak around 24 h after starting the culture. ET-1 secreted from macrophage-like synoviocytes may be involved in the pathogenesis of inflammatory arthritis.

Role of Synovial Fluid Constituents on Tribological Behavior of Synovial Joints - Application for Developing Novel Therapeutic Substitutes

2014 ◽  
Vol 658 ◽  
pp. 477-482
Author(s):  
Mirela Maria Sava ◽  
Dana Mihaela Suflet ◽  
Yves Berthier ◽  
Ana Maria Trunfio-Sfarghiu
Keyword(s):  
Synovial Fluid ◽  
Lipid Bilayers ◽  
Tribological Behavior ◽  
Ex Vivo ◽  
Structural Complexity ◽  
Joint Diseases ◽  
Major Health Problem ◽  
Synovial Joints ◽  
Lubricating Properties

Joint diseases represent a major health problem because they evolve towards the wear of cartilage for which no treatment is really effective. The difficulties in identifying the causes of these diseases are related to the biochemical and structural complexity of synovial fluid that allow cartilage lubrication. Thereby, recent studies show that synovial fluid contains micro-vesicles filled with a glycoprotein gel (hyaluronic acid and protein) surrounded by stacks of lipid bilayers which gives it excellent lubricating properties [1]. On the other side, this stable structure in vivo, becomes unstable in the ex vivo conditions. In this context, the study aims to tests the lubricating properties of synovial fluid constituents in order to identify their role on the tribological behavior of synovial joints and to develop a biomimetic synovial fluid using self-assembly of polyelectrolytes in order to stabilize the ex vivo structure for the potential therapeutic synovial fluid substitutes.Our results show that the new synthetized polysaccharides influence the friction coefficient, the stability and the wear of lipids bilayers, providing lubricating properties superior to synovial fluid biological constituents. In addition, their capacity to be structured in micro-spheres and included in lipid micro-vesicles indicates them as good biomimetic lubricants. This will allow a longer stability of synovial fluid substitutes in ex-vivo conditions in order to achieve the best lubricating properties and to improve the joint diseases treatment.

Tribological Behavior and Mechanical Properties of Graphene/PEEK Composites

2017 ◽  
Vol 5 ◽  
Author(s):  
J. Morris ◽  
R. Ríos ◽  
M. Castro ◽  
J.A. Puértolas
Keyword(s):  
Mechanical Properties ◽  
Tribological Behavior ◽  
Mechanical Resistance ◽  
Intrinsic Stiffness ◽  
Lubricating Properties

PEEK has been implemented in various orthopaedic applications due to its many beneficial mechanical, tribological, and radiolucent properties. These properties can be further tailored by introducing fillers into the PEEK matrix. In this work, graphene has been incorporated due to its high mechanical resistance, intrinsic stiffness, and lubricating properties [2].

scholarly journals The Efficiency of Conservative Methods of the Treatment at Early Stages of Osteoarthritis

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Biochemical Properties ◽  
Knee Joints ◽  
Loss Of Function ◽  
Chronic Disorder ◽  
Injectable Treatment ◽  
New Treatment ◽  
New Treatments ◽  
Lubricating Properties

Osteoarthritis (OA) is one of the leading causes of disability in the elder population. OA is a chronic disorder characterized by joint pain and inflammation, increasing physical disability and continuous cartilage degeneration.The changes of lubricating properties of synovial fluid lead to significant pain and loss of function. One of the strategies uses the high molecular weight molecule hyaluronic acid as either an injectable treatment. The injection of hyaluronic acid in the joints, improves the biochemical properties of synovial fluid into osteoarthritis of knee joints. The clinical effect is pain relief and disease modifying activity. Hyaluronic acid is a relatively new treatment that has shown varied results through several clinical trials. It can be used as a scaffold for engineering new treatments and several new preparations have been just added to the markets.

scholarly journals Properties of Journal Bearing Materials That Determine Their Wear Resistance on the Example of Aluminum-Based Alloys

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 535
Author(s):  
Alexander Mironov ◽  
Iosif Gershman ◽  
Eugeniy Gershman ◽  
Pavel Podrabinnik ◽  
Ekaterina Kuznetsova ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Wear Resistance ◽  
Journal Bearing ◽  
Tribological Behavior ◽  
Secondary Structures ◽  
Magnesium Content ◽  
Wear Rates ◽  
Relative Extension ◽  
Bearing Materials ◽  
Antifriction Alloys

Potential relations of tribological characteristics of aluminum antifriction alloys with their compositions and mechanical properties were investigated. In this regard, the properties of eight aluminum alloys containing tin from 5.4% to 11% doped with lead, copper, silicon, zinc, magnesium, and titanium were studied. Mechanical properties such as hardness, strength, relative extension, and impact strength were analyzed. Within the tribological tests seizure load and wear of material were evaluated and secondary structures were studied afterwards. The absence of a definitive correlation between tribological behavior and mechanical properties was shown. It was determined that doping tin over 6% is excessive. The seizure load of the alloys increases with the magnesium content. Secondary structures of the alloys with higher wear rates contain one order less magnesium and tin.

scholarly journals Hyaluronic Acid in Synovial Fluid

1970 ◽  
Vol 11 (2) ◽  
pp. 139-155 ◽  
Author(s):  
Nils W. Rydell ◽  
Judson Butler ◽  
Endre A. Balazs
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid

scholarly journals Analysis of Chemisorbed Tribo-Film for Ceramic-on-Ceramic Hip Joint Prostheses by Raman Spectroscopy

2021 ◽  
Vol 12 (2) ◽  
pp. 29
Author(s):  
Risha Rufaqua ◽  
Martin Vrbka ◽  
Dušan Hemzal ◽  
Dipankar Choudhury ◽  
David Rebenda ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Hyaluronic Acid ◽  
Hip Joint ◽  
Total Joint Replacement ◽  
Film Formation ◽  
Frictional Coefficient ◽  
Synovial Fluids ◽  
Frictional Coefficients ◽  
Joint Prostheses ◽  
Ceramic On Ceramic

To understand the possible lubricant mechanism in ceramic-on-ceramic hip joint prostheses, biochemical reactions of the synovial fluid and the corresponding frictional coefficients were studied. The experiments were performed in a hip joint simulator using the ball-on-cup configuration with balls and cups made from two types of ceramics, BIOLOX®forte and BIOLOX®delta. Different lubricants, namely albumin, γ-globulin, hyaluronic acid and three model synovial fluids, were studied in the experiments and Raman spectroscopy was used to analyze the biochemical responses of these lubricants at the interface. BIOLOX®delta surface was found less reactive to proteins and model fluid lubricants. In contrast, BIOLOX®forte ball surface has shown chemisorption with both proteins, hyaluronic acid and model fluids imitating total joint replacement and osteoarthritic joint. There was no direct correlation between the measured frictional coefficient and the observed chemical reactions. In summary, the study reveals chemistry of lubricant film formation on ceramic hip implant surfaces with various model synovial fluids and their components.

scholarly journals The Role of Hyaluronic Acid in Cartilage Boundary Lubrication

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1606 ◽  
Author(s):  
Weifeng Lin ◽  
Zhang Liu ◽  
Nir Kampf ◽  
Jacob Klein
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Surface Force ◽  
Force Balance ◽  
New Paradigm ◽  
Low Friction ◽  
Cartilage Surface ◽  
Boundary Lubricant ◽  
Lipid Layers

Hydration lubrication has emerged as a new paradigm for lubrication in aqueous and biological media, accounting especially for the extremely low friction (friction coefficients down to 0.001) of articular cartilage lubrication in joints. Among the ensemble of molecules acting in the joint, phosphatidylcholine (PC) lipids have been proposed as the key molecules forming, in a complex with other molecules including hyaluronic acid (HA), a robust layer on the outer surface of the cartilage. HA, ubiquitous in synovial joints, is not in itself a good boundary lubricant, but binds the PC lipids at the cartilage surface; these, in turn, massively reduce the friction via hydration lubrication at their exposed, highly hydrated phosphocholine headgroups. An important unresolved issue in this scenario is why the free HA molecules in the synovial fluid do not suppress the lubricity by adsorbing simultaneously to the opposing lipid layers, i.e., forming an adhesive, dissipative bridge between them, as they slide past each other during joint articulation. To address this question, we directly examined the friction between two hydrogenated soy PC (HSPC) lipid layers (in the form of liposomes) immersed in HA solution or two palmitoyl–oleoyl PC (POPC) lipid layers across HA–POPC solution using a surface force balance (SFB). The results show, clearly and surprisingly, that HA addition does not affect the outstanding lubrication provided by the PC lipid layers. A possible mechanism indicated by our data that may account for this is that multiple lipid layers form on each cartilage surface, so that the slip plane may move from the midplane between the opposing surfaces, which is bridged by the HA, to an HA-free interface within a multilayer, where hydration lubrication is freely active. Another possibility suggested by our model experiments is that lipids in synovial fluid may complex with HA, thereby inhibiting the HA molecules from adhering to the lipids on the cartilage surfaces.

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