scholarly journals Microstructural Parameter-Based Modeling for Transport Properties of Collagen Matrices

2015 ◽  
Vol 137 (6) ◽  
Author(s):  
Seungman Park ◽  
Catherine Whittington ◽  
Sherry L. Voytik-Harbin ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Transport Properties ◽  
Fluid Velocity ◽  
Building Blocks ◽  
Computational Method ◽  
Collagen Gels ◽  
Microstructural Parameter ◽  
Collagen Matrices ◽  
Branching Points ◽  
Matrices For Tissue Engineering

Recent advances in modulating collagen building blocks enable the design and control of the microstructure and functional properties of collagen matrices for tissue engineering and regenerative medicine. However, this is typically achieved by iterative experimentations and that process can be substantially shortened by computational predictions. Computational efforts to correlate the microstructure of fibrous and/or nonfibrous scaffolds to their functionality such as mechanical or transport properties have been reported, but the predictability is still significantly limited due to the intrinsic complexity of fibrous/nonfibrous networks. In this study, a new computational method is developed to predict two transport properties, permeability and diffusivity, based on a microstructural parameter, the specific number of interfibril branching points (or branching points). This method consists of the reconstruction of a three-dimensional (3D) fibrous matrix structure based on branching points and the computation of fluid velocity and solute displacement to predict permeability and diffusivity. The computational results are compared with experimental measurements of collagen gels. The computed permeability was slightly lower than the measured experimental values, but diffusivity agreed well. The results are further discussed by comparing them with empirical correlations in the literature for the implication for predictive engineering of collagen matrices for tissue engineering applications.

Morphology of collagen matrices for tissue engineering (biocompatibility, biodegradation, tissue response)

2015 ◽  
Vol 77 (6) ◽  
pp. 29 ◽  
Author(s):  
A. B. Shekhter ◽  
A. E. Guller ◽  
L. P. Istranov ◽  
E. V. Istranova ◽  
D. V. Butnaru ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Response ◽  
Collagen Matrices ◽  
Matrices For Tissue Engineering

Influence of Collagen Status on Microstructures of Porous Collagen/TCP Composites

2007 ◽  
Vol 330-332 ◽  
pp. 495-498
Author(s):  
Chao Zou ◽  
Wen Jian Weng ◽  
Xu Liang Deng ◽  
Kui Cheng ◽  
Pi Yi Du ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Tricalcium Phosphate ◽  
Freeze Drying ◽  
Alkali Treatment ◽  
Test Results ◽  
Collagen Matrices ◽  
The Difference ◽  
Porous Composites ◽  
Better Than

Two starting collagens, sponge and floc collagen, were used to prepare collagen/tricalcium phosphate (TCP) composites. The resulting composites were porous and had 200μm pore size. However, there was a difference in the microstructure of the pore walls for the composites derived from the two collagens, the pore walls in sponge collagen/TCP composite were still porous and had 200 nm micropores size, TCP particles were trapped in collagen matrices. While floc collagen/TCP composite had smooth and dense walls in which TCP particles were embedded. The difference could be attributed to the starting collagen with different status. Sponge collagen has a soft structure, which easily becomes disassembled fibrils during alkali treatment, the disassembled fibrils are integrated again to form a dense morphology for pore walls after freeze-drying. While floc collagen has already a low disassembly degree, the alkali treatment could not be able to separate the fibrils, this remains as micropores in pore walls after freeze-drying. Both porous composites are significant in bone tissue engineering or regeneration. MTT test results showed the two composites had good cytocompatibility, and sponge collagen/TCP composite was somewhat better than floc collagen/TCP composite, which could result from that micropores derived roughness in pore walls of sponge collagen/TCP composite is suitable for cell growth.

scholarly journals Tuning Transport Properties in Thermoelectric Nanocomposites through Inorganic Ligands and Heterostructured Building Blocks

ACS Nano ◽  
2019 ◽  
Vol 13 (6) ◽  
pp. 6572-6580 ◽  
Author(s):  
Maria Ibáñez ◽  
Aziz Genç ◽  
Roger Hasler ◽  
Yu Liu ◽  
Oleksandr Dobrozhan ◽  
...  
Keyword(s):  
Transport Properties ◽  
Building Blocks ◽  
Inorganic Ligands ◽  
Thermoelectric Nanocomposites

scholarly journals Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

2020 ◽  
Vol 24 (10) ◽  
pp. 3425-3436 ◽  
Author(s):  
Sebastian Blatt ◽  
Valentin Burkhardt ◽  
Peer W. Kämmerer ◽  
Andreas M. Pabst ◽  
Keyvan Sagheb ◽  
...  
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Staining ◽  
Collagen Matrices ◽  
Platelet Rich Fibrin ◽  
Angiogenic Potential ◽  
Branching Points ◽  
Growth Factor Release ◽  
Factor Release

Abstract Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm, jason, collprotect) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

scholarly journals Total synthesis of aStreptococcus pneumoniaeserotype 12F CPS repeating unit hexasaccharide

2017 ◽  
Vol 13 ◽  
pp. 164-173 ◽  
Author(s):  
Peter H Seeberger ◽  
Claney L Pereira ◽  
Subramanian Govindan
Keyword(s):  
Total Synthesis ◽  
Capsular Polysaccharide ◽  
Severe Disease ◽  
Building Blocks ◽  
Positive Bacterium ◽  
Linear Approach ◽  
Starting Point ◽  
Branching Points ◽  
Glycoconjugate Vaccines ◽  
Sequential Assembly

The Gram-positive bacteriumStreptococcus pneumoniaecauses severe disease globally. Vaccines that preventS. pneumoniaeinfections induce antibodies against epitopes within the bacterial capsular polysaccharide (CPS). A better immunological understanding of the epitopes that protect from bacterial infection requires defined oligosaccharides obtained by total synthesis. The key to the synthesis of theS. pneumoniaeserotype 12F CPS hexasaccharide repeating unit that is not contained in currently used glycoconjugate vaccines is the assembly of the trisaccharide β-D-GalpNAc-(1→4)-[α-D-Glcp-(1→3)]-β-D-ManpNAcA, in which the branching points are equipped with orthogonal protecting groups. A linear approach relying on the sequential assembly of monosaccharide building blocks proved superior to a convergent [3 + 3] strategy that was not successful due to steric constraints. The synthetic hexasaccharide is the starting point for further immunological investigations.

Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering

2018 ◽  
Vol 6 (3) ◽  
pp. 550-561 ◽  
Author(s):  
Koji Nagahama ◽  
Naho Oyama ◽  
Kimika Ono ◽  
Atsushi Hotta ◽  
Keiko Kawauchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Building Blocks ◽  
The Body ◽  
Bioactive Molecules ◽  
In Vivo Tissue Engineering ◽  
Injectable Gels

Nanocomposite injectable gels, which self-replenish regenerative extracellular microenvironments within the gels in the body by utilizing host-derived bioactive molecules as building blocks, are reported.

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