Models of Cell Interaction Based on Differential Adhesion

1984 ◽  
Vol 106 (1) ◽  
pp. 36-41 ◽  
Author(s):  
S. Childress
Keyword(s):  
Three Dimensional ◽  
Numerical Algorithms ◽  
Cell Types ◽  
Cell Interaction ◽  
Cell Aggregates ◽  
Biological Cells ◽  
Mechanical Interaction ◽  
Cell Sheets ◽  
Differential Adhesion ◽  
Dimensional Cell

Recent efforts to model the mechanical interaction of aggregates of biological cells are reviewed. Differential adhesion is discussed as a means of creating a field of stress equivalent to tension elements at interfaces between unlike cell types. Several numerical algorithms are described and applied to shortening and folding of cell sheets, three-dimensional monolayers, and two-dimensional cell aggregates.

scholarly journals In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair

2018 ◽  
Vol 19 (8) ◽  
pp. 2272 ◽  
Author(s):  
Chi-Fen Hsieh ◽  
Zexing Yan ◽  
Ricarda Schumann ◽  
Stefan Milz ◽  
Christian Pfeifer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Sheet ◽  
Tendon Repair ◽  
Three Dimensional ◽  
Cell Types ◽  
Tendon Tissue ◽  
Cell Sheets ◽  
Tendon Tissue Engineering ◽  
Dimensional Cell

The poor and slow healing capacity of tendons requires novel strategies to speed up the tendon repair process. Hence, new and promising developments in tendon tissue engineering have become increasingly relevant. Previously, we have established a tendon progenitor cell line via ectopic expression of the tendon-related basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) in human bone marrow mesenchymal stem cells (hMSC-Scx). The aim of this study was to directly compare the characteristics of hMSC-Scx cells to that of primary human tendon stem/progenitors cells (hTSPCs) via assessment of self-renewal and multipotency, gene marker expression profiling, in vitro wound healing assay and three-dimensional cell sheet formation. As expected, hTSPCs were more naive than hMSC-Scx cells because of higher clonogenicity, trilineage differentiation potential, and expression of stem cell markers, as well as higher mRNA levels of several gene factors associated with early tendon development. Interestingly, with regards to wound healing, both cell types demonstrate a comparable speed of scratch closure, as well as migratory velocity and distance in various migration experiments. In the three-dimensional cell sheet model, hMSC-Scx cells and hTSPCs form compact tendinous sheets as histological staining, and transmission electron microscopy shows spindle-shaped cells and collagen type I fibrils with similar average diameter size and distribution. Taken together, hTSPCs exceed hMSC-Scx cells in several characteristics, namely clonogenicity, multipotentiality, gene expression profile and rates of tendon-like sheet formation, whilst in three-dimensional cell sheets, both cell types have comparable in vitro healing potential and collagenous composition of their three-dimensional cell sheets, making both cell types a suitable cell source for tendon tissue engineering and healing.

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

Three-dimensional cell aggregates composed of HUVECs and cbMSCs for therapeutic neovascularization in a mouse model of hindlimb ischemia

Biomaterials ◽  
2013 ◽  
Vol 34 (8) ◽  
pp. 1995-2004 ◽  
Author(s):  
Ding-Yuan Chen ◽  
Hao-Ji Wei ◽  
Kun-Ju Lin ◽  
Chieh-Cheng Huang ◽  
Chung-Chi Wang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Hindlimb Ischemia ◽  
Dimensional Cell

scholarly journals A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 179-189 ◽  
Author(s):  
Andreas R. Thomsen ◽  
Christine Aldrian ◽  
Peter Bronsert ◽  
Yi Thomann ◽  
Norbert Nanko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Volume Measurement ◽  
Cell Aggregates ◽  
Microwell Array ◽  
Dimensional Cell

Miniaturised conical measures for cell aggregates.

Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds

2019 ◽  
Vol 48 (3) ◽  
pp. 968-979
Author(s):  
Yan Liang ◽  
Alexander R. A. Szojka ◽  
Enaam Idrees ◽  
Melanie Kunze ◽  
Aillette Mulet-Sierra ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Aggregates ◽  
Dimensional Cell

scholarly journals Modelling molecular mechanisms of breast cancer and invasion: lessons from the normal gland

2007 ◽  
Vol 35 (1) ◽  
pp. 18-22 ◽  
Author(s):  
M.J. Bissell
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Cell Types ◽  
Genes And Environment ◽  
Different Cell Types ◽  
Cancerous Cells ◽  
Cancer Studies ◽  
Dimensional Cell

The interplay between genes and environment is complex, particularly when it comes to cancer. Studies on breast cancer cells have shown that environmental influences dominate over genotype in their effect on phenotype, and can cause cancerous cells to revert to a non-malignant phenotype, while remaining genotypically malignant. Using breast tissue in three-dimensional cell culture has proved a better model than traditional two-dimensional cell culture in that different cell types can be seen to behave differently to the same pro­-apoptotic signal, with normal cells surviving.

scholarly journals The Anti-Proliferative Effect of PI3K/mTOR and ERK Inhibition in Monolayer and Three-Dimensional Ovarian Cancer Cell Models

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 395
Author(s):  
Elizabeth Dunn ◽  
Kenny Chitcholtan ◽  
Peter Sykes ◽  
Ashley Garrill
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Alternative Pathway ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Erk Inhibition ◽  
Inhibitor Combination ◽  
Dimensional Cell

Most ovarian cancer patients are diagnosed with advanced stage disease, which becomes unresponsive to chemotherapeutic treatments. The PI3K/AKT/mTOR and the RAS/RAF/MEK/ERK kinase signaling pathways are attractive targets for potential therapeutic inhibitors, due to the high frequency of mutations to PTEN, PIK3CA, KRAS and BRAF in several ovarian cancer subtypes. However, monotherapies targeting one of these pathways have shown modest effects in clinical trials. This limited efficacy of the agents could be due to upregulation and increased signaling via the adjacent alternative pathway. In this study, the efficacy of combined PI3K/mTOR (BEZ235) and ERK inhibition (SCH772984) was investigated in four human ovarian cancer cell lines, grown as monolayer and three-dimensional cell aggregates. The inhibitor combination reduced cellular proliferation in a synergistic manner in OV-90 and OVCAR8 monolayers and in OV-90, OVCAR5 and SKOV3 aggregates. Sensitivity to the inhibitors was reduced in three-dimensional cell aggregates in comparison to monolayers. OV-90 cells cultured in large spheroids were sensitive to the inhibitors and displayed a robust synergistic antiproliferative response to the inhibitor combination. In contrast, OVCAR8 spheroids were resistant to the inhibitors. These findings suggest that combined PI3K/mTOR and ERK inhibition could be a useful strategy for overcoming treatment resistance in ovarian cancer and warrants further preclinical investigation. Additionally, in some cell lines the use of different three-dimensional models can influence cell line sensitivity to PI3K/mTOR and RAS/RAF/MEK/ERK pathway inhibitors.

scholarly journals 3D cell sheet structure augments mesenchymal stem cell cytokine production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sophia Bou-Ghannam ◽  
Kyungsook Kim ◽  
David W. Grainger ◽  
Teruo Okano
Keyword(s):  
Growth Factor ◽  
Connexin 43 ◽  
Cell Sheet ◽  
Three Dimensional ◽  
3D Culture ◽  
Cell Interaction ◽  
Interleukin 10 ◽  
Free Cell ◽  
Cell Sheets ◽  
Paracrine Factors

AbstractMesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation in three-dimensional (3D) tissue-like microenvironments. Toward this end, this study explored scaffold-free cell sheet technology as a new 3D platform. MSCs cultivated on temperature-responsive culture dishes to a confluent 2D monolayer were harvested by temperature reduction from 37 to 20 °C that induces a surface wettability transition from hydrophobic to hydrophilic. Release of culture-adherent tension induced spontaneous cell sheet contraction, reducing the diameter 2.4-fold, and increasing the thickness 8.0-fold to render a 3D tissue-like construct with a 36% increase in tissue volume. This 2D-to-3D transition reorganized MSC actin cytoskeleton from aligned to multidirectional, corresponding to a cell morphological change from elongated in 2D monolayers to rounded in 3D cell sheets. 3D culture increased MSC gene expression of cell interaction proteins, β-catenin, integrin β1, and connexin 43, and of pro-tissue regenerative cytokines, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10), and increased VEGF secretion per MSC 2.1-fold relative to 2D cultures. Together, these findings demonstrate that MSC therapeutic potency can be enhanced by 3D cell sheet tissue structure.

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