Label-Free and Near-Field Mapping of Molecular Diffusion (Saline Solution/Water) Using Surface Plasmon Resonance (SPR) Refractive Index Field Imaging

2008 ◽  
Vol 130 (8) ◽  
Author(s):  
Il Tai Kim ◽  
Kenneth D. Kihm
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Diffusion ◽  
Saline Solution ◽  
Near Field ◽  
Label Free ◽  
Field Mapping ◽  
Field Imaging

Advances in surface plasmon resonance biosensing research

Nanoscale ◽  
2021 ◽  
Author(s):  
Qi Wang ◽  
Zihan Ren ◽  
Wan-Ming Zhao ◽  
Lei Wang ◽  
Xin Yan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Label Free ◽  
Surface Plasmon Reso

The surface plasmon reso-nance (SPR) phenomenon is of wide interest for its sen-sitivity to changes in sur-face refractive index for label-free, high sensitivity and rapid detection of bi-omarkers. This paper...

Label-Free Protein Detection via Gold Nanoparticles and Localized Surface Plasmon Resonance

2009 ◽  
Vol 74 ◽  
pp. 95-98 ◽  
Author(s):  
Shao Li Zhu ◽  
Jing Bo Zhang ◽  
Lanry Yung Lin Yue ◽  
Dany Hartono ◽  
Ai Qun Liu
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Protein Detection ◽  
Glass Slide ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Ultrapure Water

Protein plays key role in cellular processes and diseases diagnosis [1-3]. This paper reports a label-free detection of proteins through localized surface plasmon resonance (LSPR) and realized Au nanoparticles (AuNPs) immobilized on glass slide. Glass slide is first sonicated with 5% v/v glassware detergent in ultrapure water for 30 mins, and then in ultrapure water for another 30 mins. A 5% v/v aminopropyltriethoxysilane (APTES) solution in ultrapure water is prepared and the glass slide is immersed in the 5% v/v APTES solution for 20 mins. The glass slide is sonicated for 60 mins in ultrapure water. The purpose of the sonication is to remove the multi-layers of APTES which are formed on the glass surface. The silanized glass slide is immersed in AuNPs solution for 40 mins. Finally, the protein solution is added on the surface of the slide. The surface absorption of proteins induces a significant difference of environmental refractive index around the AuNPs. The shift in the wavelength of the LSPR spectra is very sensitive to the change in the surface refractive index of the nanoparticles. The shifts in the LSPR spectra are primarily determined by the volatility and refractive indices of the protein species. In this paper, the LSPR-nanobiosensor is designed and fabricated. The LSPR band responses are measured by a real-time UV–vis spectrometer with a CCD array detector. The response time of the protein–LSPR spectrum is less than 3 seconds and the response is reversible and reproducible.

scholarly journals Plasmonic nano-aperture label-free imaging (PANORAMA)

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nareg Ohannesian ◽  
Ibrahim Misbah ◽  
Steven H. Lin ◽  
Wei-Chuan Shih
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Near Field ◽  
Dark Field ◽  
Localized Surface Plasmon ◽  
Wide Field ◽  
Label Free ◽  
Surface Sensitivity ◽  
Surface Localization

AbstractLabel-free optical imaging of nanoscale objects faces fundamental challenges. Techniques based on propagating surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) have shown promises. However, challenges remain to achieve diffraction-limited resolution and better surface localization in SPR imaging. LSPR imaging with dark-field microscopy on metallic nanostructures suffers from low light throughput and insufficient imaging capacity. Here we show ultra-near-field index modulated PlAsmonic NanO-apeRture lAbel-free iMAging (PANORAMA) which uniquely relies on unscattered light to detect sub-100 nm dielectric nanoparticles. PANORAMA provides diffraction-limited resolution, higher surface sensitivity, and wide-field imaging with dense spatial sampling. Its system is identical to a standard bright-field microscope with a lamp and a camera – no laser or interferometry is needed. In a parallel fashion, PANORAMA can detect, count and size individual dielectric nanoparticles beyond 25 nm, and dynamically monitor their distance to the plasmonic surface at millisecond timescale.

scholarly journals Surface Plasmon Resonance Imaging of Excitable Cells

2018 ◽  
Author(s):  
Carmel L. Howe ◽  
Kevin F. Webb ◽  
Sidahmed A. Abayzeed ◽  
David J. Anderson ◽  
Chris Denning ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Numerical Aperture ◽  
Imaging Systems ◽  
Live Cells ◽  
Objective Lens ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free

AbstractSurface plasmons are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detects temporal variations in the refractive index at a point. SPR has also been used in imaging systems where the spatial variations of refractive index in the sample provide the contrast mechanism. A high numerical aperture objective lens has been used to design SPR microscopy systems with the ability to image adherent live cells. Addressing research questions in cell physiology and pharmacology often requires the development of a multimodal microscope where complementary information can be obtained.In this paper, we present the development of a multimodal microscope that combines surface plasmon resonance imaging with a number of additional imaging modalities including bright-field, epi-fluorescence, total internal reflection microscopy (TIRM) and SPR fluorescence microscopy. We used a high numerical aperture objective lens to achieve SPR and TIR microscopy with the ability to image adherent live cells non-invasively. The platform has been used to image live cell cultures demonstrating both fluorescent and label-free techniques. The SPR and TIR imaging systems feature a wide field of view (300 µm) that allows measurements from multiple cells while the resolution is sufficient to image fine cellular processes. The ability of the platform to perform label-free functional imaging of living cell was demonstrated by imaging the spatial variations in contraction of stem cell-derived cardiomyocytes. This technique has a promise for non-invasive imaging of the development of cultured cells over very long periods of time.

scholarly journals Optical Characterization of Ultra-Thin Films of Azo-Dye-Doped Polymers Using Ellipsometry and Surface Plasmon Resonance Spectroscopy

Photonics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 41
Author(s):  
Najat Andam ◽  
Siham Refki ◽  
Hidekazu Ishitobi ◽  
Yasushi Inouye ◽  
Zouheir Sekkat
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Spectroscopic Ellipsometry ◽  
Plasmon Resonance ◽  
Complex Refractive Index ◽  
Resonance Spectroscopy ◽  
Doped Polymers

The determination of optical constants (i.e., real and imaginary parts of the complex refractive index (nc) and thickness (d)) of ultrathin films is often required in photonics. It may be done by using, for example, surface plasmon resonance (SPR) spectroscopy combined with either profilometry or atomic force microscopy (AFM). SPR yields the optical thickness (i.e., the product of nc and d) of the film, while profilometry and AFM yield its thickness, thereby allowing for the separate determination of nc and d. In this paper, we use SPR and profilometry to determine the complex refractive index of very thin (i.e., 58 nm) films of dye-doped polymers at different dye/polymer concentrations (a feature which constitutes the originality of this work), and we compare the SPR results with those obtained by using spectroscopic ellipsometry measurements performed on the same samples. To determine the optical properties of our film samples by ellipsometry, we used, for the theoretical fits to experimental data, Bruggeman’s effective medium model for the dye/polymer, assumed as a composite material, and the Lorentz model for dye absorption. We found an excellent agreement between the results obtained by SPR and ellipsometry, confirming that SPR is appropriate for measuring the optical properties of very thin coatings at a single light frequency, given that it is simpler in operation and data analysis than spectroscopic ellipsometry.

scholarly journals Numerical Study on the Surface Plasmon Resonance Tunability of Spherical and Non-Spherical Core-Shell Dimer Nanostructures

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1728
Author(s):  
Joshua Fernandes ◽  
Sangmo Kang
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Surface Plasmon Resonance ◽  
Aspect Ratio ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Shell Thickness ◽  
Numerical Study ◽  
Field Enhancement ◽  
Core Shell

The near-field enhancement and localized surface plasmon resonance (LSPR) on the core-shell noble metal nanostructure surfaces are widely studied for various biomedical applications. However, the study of the optical properties of new plasmonic non-spherical nanostructures is less explored. This numerical study quantifies the optical properties of spherical and non-spherical (prolate and oblate) dimer nanostructures by introducing finite element modelling in COMSOL Multiphysics. The surface plasmon resonance peaks of gold nanostructures should be understood and controlled for use in biological applications such as photothermal therapy and drug delivery. In this study, we find that non-spherical prolate and oblate gold dimers give excellent tunability in a wide range of biological windows. The electromagnetic field enhancement and surface plasmon resonance peak can be tuned by varying the aspect ratio of non-spherical nanostructures, the refractive index of the surrounding medium, shell thickness, and the distance of separation between nanostructures. The absorption spectra exhibit considerably greater dependency on the aspect ratio and refractive index than the shell thickness and separation distance. These results may be essential for applying the spherical and non-spherical nanostructures to various absorption-based applications.

Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Localized Surface Plasmon ◽  
Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

scholarly journals Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4348
Author(s):  
Piotr Mrozek ◽  
Ewa Gorodkiewicz ◽  
Paweł Falkowski ◽  
Bogusław Hościło
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Real Life ◽  
Reflectance Measurement ◽  
Near Surface ◽  
Bimetallic Surface ◽  
Calibration Curves ◽  
Surface Sensing

Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations.

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