scholarly journals Measurement of the Dynamic Shear Modulus of Mouse Brain Tissue In Vivo by Magnetic Resonance Elastography

2008 ◽  
Vol 130 (2) ◽  
Author(s):  
Stefan M. Atay ◽  
Christopher D. Kroenke ◽  
Arash Sabet ◽  
Philip V. Bayly
Keyword(s):  
Magnetic Resonance ◽  
Shear Modulus ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Ex Vivo ◽  
Three Dimensions ◽  
Dynamic Shear Modulus ◽  
Dynamic Shear ◽  
High Deformation

In this study, the magnetic resonance (MR) elastography technique was used to estimate the dynamic shear modulus of mouse brain tissue in vivo. The technique allows visualization and measurement of mechanical shear waves excited by lateral vibration of the skull. Quantitative measurements of displacement in three dimensions during vibration at 1200Hz were obtained by applying oscillatory magnetic field gradients at the same frequency during a MR imaging sequence. Contrast in the resulting phase images of the mouse brain is proportional to displacement. To obtain estimates of shear modulus, measured displacement fields were fitted to the shear wave equation. Validation of the procedure was performed on gel characterized by independent rheometry tests and on data from finite element simulations. Brain tissue is, in reality, viscoelastic and nonlinear. The current estimates of dynamic shear modulus are strictly relevant only to small oscillations at a specific frequency, but these estimates may be obtained at high frequencies (and thus high deformation rates), noninvasively throughout the brain. These data complement measurements of nonlinear viscoelastic properties obtained by others at slower rates, either ex vivo or invasively.

Magnetic Resonance Elastography of the Mouse Brain In Vivo

2007 ◽  
Author(s):  
Stefan M. Atay ◽  
Philip V. Bayly
Keyword(s):  
Magnetic Resonance ◽  
Shear Modulus ◽  
Mouse Brain ◽  
Ex Vivo ◽  
Magnetic Resonance Elastography ◽  
Dynamic Shear Modulus ◽  
Dynamic Shear ◽  
High Deformation ◽  
Nonlinear Viscoelastic

In the current study we apply the magnetic resonance elastography (MRE) technique to estimate the dynamic shear modulus of mouse brain tissue in vivo. The frequency used (1200 Hz) is well above those reported previously [1]. Estimates of dynamic shear modulus range from 12,600–14,800 N/m2 at 1200 Hz. These data are strictly relevant only to small oscillations at this specific frequency, but these values are obtained at high frequencies (and thus high deformation rates) and non-invasively throughout the brain. These data complement measurements of nonlinear viscoelastic properties obtained by others at slower rates, either ex vivo or invasively.

scholarly journals Multiple Irradiation Affects Cellular and Extracellular Components of the Mouse Brain Tissue and Adhesion and Proliferation of Glioblastoma Cells in Experimental System In Vivo

2021 ◽  
Vol 22 (24) ◽  
pp. 13350
Author(s):  
Maxim O. Politko ◽  
Alexandra Y. Tsidulko ◽  
Oxana A. Pashkovskaya ◽  
Konstantin E. Kuper ◽  
Anastasia V. Suhovskih ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Mouse Brain ◽  
Glial Cells ◽  
Ex Vivo ◽  
Experimental System ◽  
Normal Brain ◽  
Biosynthetic Activity ◽  
Normal Brain Tissue

Intensive adjuvant radiotherapy (RT) is a standard treatment for glioblastoma multiforme (GBM) patients; however, its effect on the normal brain tissue remains unclear. Here, we investigated the short-term effects of multiple irradiation on the cellular and extracellular glycosylated components of normal brain tissue and their functional significance. Triple irradiation (7 Gy*3 days) of C57Bl/6 mouse brain inhibited the viability, proliferation and biosynthetic activity of normal glial cells, resulting in a fast brain-zone-dependent deregulation of the expression of proteoglycans (PGs) (decorin, biglycan, versican, brevican and CD44). Complex time-point-specific (24–72 h) changes in decorin and brevican protein and chondroitin sulfate (CS) and heparan sulfate (HS) content suggested deterioration of the PGs glycosylation in irradiated brain tissue, while the transcriptional activity of HS-biosynthetic system remained unchanged. The primary glial cultures and organotypic slices from triple-irradiated brain tissue were more susceptible to GBM U87 cells’ adhesion and proliferation in co-culture systems in vitro and ex vivo. In summary, multiple irradiation affects glycosylated components of normal brain extracellular matrix (ECM) through inhibition of the functional activity of normal glial cells. The changed content and pattern of PGs and GAGs in irradiated brain tissues are accompanied by the increased adhesion and proliferation of GBM cells, suggesting a novel molecular mechanism of negative side-effects of anti-GBM radiotherapy.

scholarly journals Automated Segmentation of in Vivo and Ex Vivo Mouse Brain Magnetic Resonance Images

2009 ◽  
Vol 8 (1) ◽  
pp. 7290.2009.00004 ◽  
Author(s):  
Alize E.H. Scheenstra ◽  
Rob C.G. van de Ven ◽  
Louise van der Weerd ◽  
Arn M.J.M. van den Maagdenberg ◽  
Jouke Dijkstra ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Mouse Brain ◽  
Ex Vivo ◽  
Magnetic Resonance Images ◽  
Automated Segmentation

Monocytes as Carriers of Magnetic Nanoparticles for Tracking Inflammation in the Epileptic Rat Brain

2019 ◽  
Vol 16 (7) ◽  
pp. 637-644 ◽  
Author(s):  
Hadas Han ◽  
Sara Eyal ◽  
Emma Portnoy ◽  
Aniv Mann ◽  
Miriam Shmuel ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Ex Vivo ◽  
In Vivo Studies ◽  
Nanoparticle Distribution ◽  
Spontaneous Seizures ◽  
Systemic Distribution ◽  
Hippocampal Ca1 ◽  
Pilocarpine Model

Background: Inflammation is a hallmark of epileptogenic brain tissue. Previously, we have shown that inflammation in epilepsy can be delineated using systemically-injected fluorescent and magnetite- laden nanoparticles. Suggested mechanisms included distribution of free nanoparticles across a compromised blood-brain barrier or their transfer by monocytes that infiltrate the epileptic brain. Objective: In the current study, we evaluated monocytes as vehicles that deliver nanoparticles into the epileptic brain. We also assessed the effect of epilepsy on the systemic distribution of nanoparticleloaded monocytes. Methods: The in vitro uptake of 300-nm nanoparticles labeled with magnetite and BODIPY (for optical imaging) was evaluated using rat monocytes and fluorescence detection. For in vivo studies we used the rat lithium-pilocarpine model of temporal lobe epilepsy. In vivo nanoparticle distribution was evaluated using immunohistochemistry. Results: 89% of nanoparticle loading into rat monocytes was accomplished within 8 hours, enabling overnight nanoparticle loading ex vivo. The dose-normalized distribution of nanoparticle-loaded monocytes into the hippocampal CA1 and dentate gyrus of rats with spontaneous seizures was 176-fold and 380-fold higher compared to the free nanoparticles (p<0.05). Seizures were associated with greater nanoparticle accumulation within the liver and the spleen (p<0.05). Conclusion: Nanoparticle-loaded monocytes are attracted to epileptogenic brain tissue and may be used for labeling or targeting it, while significantly reducing the systemic dose of potentially toxic compounds. The effect of seizures on monocyte biodistribution should be further explored to better understand the systemic effects of epilepsy.

Shear Modulus and Damping Ratio of Gravel Material

2011 ◽  
Vol 105-107 ◽  
pp. 1426-1432 ◽  
Author(s):  
De Gao Zou ◽  
Tao Gong ◽  
Jing Mao Liu ◽  
Xian Jing Kong
Keyword(s):  
Shear Modulus ◽  
Shear Strain ◽  
Strain Amplitude ◽  
Damping Ratio ◽  
Confining Pressure ◽  
Dynamic Shear Modulus ◽  
Test Results ◽  
Dynamic Shear ◽  
Data Points ◽  
Shear Strain Amplitude

Two of the most important parameters in dynamic analysis involving soils are the dynamic shear modulus and the damping ratio. In this study, a series of tests were performed on gravels. For comparison, some other tests carried out by other researchers were also collected. The test results show that normalized shear modulus and damping ratio vary with the shear strain amplitude, (1) normalized shear modulus decreases with the increase of dynamic shear strain amplitude, and as the confining pressure increases, the test data points move from the low end toward the high end; (2) damping ratio increases with the increase of shear strain amplitude, damping ratio is dependent on confining pressure where an increase in confining pressure decreased damping ratio. According to the test results, a reference formula is proposed to evaluate the maximum dynamic shear modulus, the best-fit curve and standard deviation bounds for the range of data points are also proposed.

scholarly journals The effect of fluid saturation on the dynamic shear modulus of tight sandstones

2017 ◽  
Vol 14 (5) ◽  
pp. 1072-1086 ◽  
Author(s):  
Dongqing Li ◽  
Jianxin Wei ◽  
Bangrang Di ◽  
Pinbo Ding ◽  
Da Shuai
Keyword(s):  
Shear Modulus ◽  
Dynamic Shear Modulus ◽  
Dynamic Shear ◽  
Fluid Saturation

Experimental Study on Dynamic Characteristics of Ionic Soil Stabilizer Reinforcing Red Clay

2011 ◽  
Vol 374-377 ◽  
pp. 1391-1395
Author(s):  
Xue Song Lu ◽  
Wei Xiang
Keyword(s):  
Shear Modulus ◽  
Shear Strain ◽  
Dynamic Condition ◽  
Damping Ratio ◽  
Confining Pressure ◽  
Natural Soil ◽  
Dynamic Shear Modulus ◽  
Dynamic Shear ◽  
Red Clay ◽  
Soil Stabilizer

Based on the red clay of Wuhan reinforced by Ionic Soil Stabilizer, the red clay soil is treated by different matches of ISS at first, then is tested in the Atterberg limits test and dynamic triaxia test. The results show that the plastic index decreases, and the red clay were greatly improved under the dynamic condition, the maximum dynamic shear modulus ratio acquired an incensement of 27.72% on average after mixing the ISS into the red clay. In addition, It was concluded that the confining pressure influenced the dynamic shear modulus and damping ratio to a certain extent. Given the same strain conditions, with the incensement of confining pressure increases, the dynamic shear modulus increased and the damping ratio decreased. Moreover, when plotting the dynamic shear modulus versus the dynamic shear strain, the similar curve can be formed for both the natural soil and the modified one, the dynamic shear modulus monotonously decreased with the incensement of the dynamic shear strain. However, the value of dynamic shear modulus differed in the same shear strain between the natural soil and the soil modified by ISS.

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