scholarly journals Temperature dependence of Na + −H + exchange, Na + −HCO 3 − co‐transport, intracellular buffering and intracellular pH in guinea‐pig ventricular myocytes

2003 ◽  
Vol 552 (3) ◽  
pp. 715-726 ◽  
Author(s):  
Frederick F.‐T. Ch'en ◽  
Emma Dilworth ◽  
Pawel Swietach ◽  
Ruth S. Goddard ◽  
Richard D. Vaughan‐Jones
Keyword(s):  
Guinea Pig ◽  
Temperature Dependence ◽  
Intracellular Ph ◽  
Ventricular Myocytes

Na-independent Cl(-)-HCO3- exchange mediates recovery of pHi from alkalosis in guinea pig ventricular myocytes

1994 ◽  
Vol 267 (1) ◽  
pp. H85-H91 ◽  
Author(s):  
P. Xu ◽  
K. W. Spitzer
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Initial Rate ◽  
Ph Sensitive ◽  
Disulfonic Acid ◽  
Weak Base ◽  
External Application ◽  
Fluorescent Indicator ◽  
Ethanesulfonic Acid

The pH-sensitive fluorescent indicator, carboxy-seminaphthorhodafluor 1 (SNARF 1) was used to assess the contribution of forward Na-independent Cl(-)-HCO3- exchange (1 external Cl- exchanged for 1 internal HCO3-) to intracellular pH (pHi) recovery from alkalosis in adult ventricular myocytes (guinea pig). Intracellular alkalosis was elicited by external application of the weak base, trimethylamine. In the absence of CO2-HCO3- (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solution) the initial rate of pHi recovery from alkalosis (pHi = 7.25-7.75) was slow and independent of pHi, yielding an apparent net HCO3- efflux of 0.36 +/- 0.11 mM/min. In CO2-HCO3(-)-buffered solution, the initial rate of pHi recovery and net HCO3- efflux were much faster and markedly increased by raising pHi. At pHi approximately 7.25, net HCO3- efflux was approximately 2 mM/min and rose to 9 mM/min at pHi approximately 7.6. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (0.4 mM) decreased net HCO3- efflux by 78.1 +/- 8.9% in CO2-HCO3(-)-buffered solution. Reduction in extracellular Cl- concentration from 135 to 20 mM markedly slowed the rate of pHi recovery from alkalosis and reduced net HCO3- efflux. pHi recovery from alkalosis was unaffected by removal of external sodium or exposure to 1 mM amiloride. These results indicate that forward Na-independent Cl(-)-HCO3- exchange mediates pHi recovery from alkalosis in guinea pig ventricular myocytes.

Effect of S20787, a novel Cl−-HCO3− exchange inhibitor, on intracellular pH regulation in guinea pig ventricular myocytes

2001 ◽  
Vol 8 (5) ◽  
pp. 395-405 ◽  
Author(s):  
Shih-Hurng Loh ◽  
Chien-Sung Tsai ◽  
Cheng-I Lin ◽  
Jong-Shiaw Jin ◽  
Richard D. Vaughan-Jones
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Ph Regulation ◽  
Intracellular Ph Regulation ◽  
Exchange Inhibitor

Effect of S20787, a Novel Cl–-HCO–3 Exchange Inhibitor, on Intracellular pH Regulation in Guinea Pig Ventricular Myocytes

2001 ◽  
Vol 8 (5) ◽  
pp. 395-405
Author(s):  
Shih-Hurng Loh ◽  
Chien-Sung Tsai ◽  
Cheng-I Lin ◽  
Jong-Shiaw Jin ◽  
Richard D. Vaughan-Jones
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Ph Regulation ◽  
Intracellular Ph Regulation ◽  
Exchange Inhibitor

scholarly journals Inhibition of Na-K pump current in guinea pig ventricular myocytes by dihydroouabain occurs at high- and low-affinity sites.

1989 ◽  
Vol 64 (6) ◽  
pp. 1063-1069 ◽  
Author(s):  
D J Mogul ◽  
H H Rasmussen ◽  
D H Singer ◽  
R E Ten Eick
Keyword(s):  
Guinea Pig ◽  
Ventricular Myocytes ◽  
Pump Current
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