scholarly journals Comparison of intracellular pH transients in single ventricular myocytes and isolated ventricular muscle of guinea-pig.

1990 ◽  
Vol 424 (1) ◽  
pp. 343-365 ◽  
Author(s):  
C Bountra ◽  
T Powell ◽  
R D Vaughan-Jones
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Ventricular Muscle ◽  
Single Ventricular Myocytes

Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system

2000 ◽  
Vol 278 (4) ◽  
pp. H1371-H1382 ◽  
Author(s):  
Kenneth W. Spitzer ◽  
Philip R. Ershler ◽  
Rita L. Skolnick ◽  
Richard D. Vaughan-Jones
Keyword(s):  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Weak Acid ◽  
Confocal Imaging ◽  
Ion Diffusion ◽  
External Application ◽  
Ph Gradients ◽  
Regional Myocardial Ischemia ◽  
Single Ventricular Myocytes ◽  
Myocyte Function

This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pHi) within single ventricular myocytes. The spatial distribution of pHi in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pHi were induced by local external application of NH4Cl, CO2, or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 μm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pHi gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should be useful for studying the effects of localized acidosis on myocyte function, estimating intracellular ion diffusion rates, and, possibly, inducing regional changes in other important intracellular ions.

5-hydroxydecanoate inhibits ATP-sensitive K+ channel currents in guinea-pig single ventricular myocytes

1992 ◽  
Vol 220 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Tatsuto Notsu ◽  
Kiyokazu Ohhashi ◽  
Isao Tanaka ◽  
Hiroshi Ishikawa ◽  
Takeshi Niho ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Ventricular Myocytes ◽  
K Channel ◽  
Single Ventricular Myocytes ◽  
Channel Currents

Na-independent Cl(-)-HCO3- exchange mediates recovery of pHi from alkalosis in guinea pig ventricular myocytes

1994 ◽  
Vol 267 (1) ◽  
pp. H85-H91 ◽  
Author(s):  
P. Xu ◽  
K. W. Spitzer
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Ventricular Myocytes ◽  
Initial Rate ◽  
Ph Sensitive ◽  
Disulfonic Acid ◽  
Weak Base ◽  
External Application ◽  
Fluorescent Indicator ◽  
Ethanesulfonic Acid

The pH-sensitive fluorescent indicator, carboxy-seminaphthorhodafluor 1 (SNARF 1) was used to assess the contribution of forward Na-independent Cl(-)-HCO3- exchange (1 external Cl- exchanged for 1 internal HCO3-) to intracellular pH (pHi) recovery from alkalosis in adult ventricular myocytes (guinea pig). Intracellular alkalosis was elicited by external application of the weak base, trimethylamine. In the absence of CO2-HCO3- (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered solution) the initial rate of pHi recovery from alkalosis (pHi = 7.25-7.75) was slow and independent of pHi, yielding an apparent net HCO3- efflux of 0.36 +/- 0.11 mM/min. In CO2-HCO3(-)-buffered solution, the initial rate of pHi recovery and net HCO3- efflux were much faster and markedly increased by raising pHi. At pHi approximately 7.25, net HCO3- efflux was approximately 2 mM/min and rose to 9 mM/min at pHi approximately 7.6. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (0.4 mM) decreased net HCO3- efflux by 78.1 +/- 8.9% in CO2-HCO3(-)-buffered solution. Reduction in extracellular Cl- concentration from 135 to 20 mM markedly slowed the rate of pHi recovery from alkalosis and reduced net HCO3- efflux. pHi recovery from alkalosis was unaffected by removal of external sodium or exposure to 1 mM amiloride. These results indicate that forward Na-independent Cl(-)-HCO3- exchange mediates pHi recovery from alkalosis in guinea pig ventricular myocytes.

scholarly journals Temperature dependence of Na + −H + exchange, Na + −HCO 3 − co‐transport, intracellular buffering and intracellular pH in guinea‐pig ventricular myocytes

2003 ◽  
Vol 552 (3) ◽  
pp. 715-726 ◽  
Author(s):  
Frederick F.‐T. Ch'en ◽  
Emma Dilworth ◽  
Pawel Swietach ◽  
Ruth S. Goddard ◽  
Richard D. Vaughan‐Jones
Keyword(s):  
Guinea Pig ◽  
Temperature Dependence ◽  
Intracellular Ph ◽  
Ventricular Myocytes
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