scholarly journals Evidence that cell surface charge reduction modifes capillary red cell velocity-flux relationships in hamster cremaster muscle.

1995 ◽  
Vol 489 (1) ◽  
pp. 193-201 ◽  
Author(s):  
H Vink ◽  
P A Wieringa ◽  
J A Spaan
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Red Cell ◽  
Charge Reduction ◽  
Cremaster Muscle ◽  
Cell Surface Charge ◽  
Cell Velocity ◽  
Red Cell Velocity

Attenuation of blood flow-induced dilation in arterioles after muscle contraction

1994 ◽  
Vol 266 (5) ◽  
pp. H2114-H2121 ◽  
Author(s):  
M. Cabel ◽  
V. Smiesko ◽  
P. C. Johnson
Keyword(s):  
Shear Rate ◽  
Muscle Contraction ◽  
Vessel Diameter ◽  
Similar Degree ◽  
Red Cell ◽  
Cremaster Muscle ◽  
Third Order ◽  
Cell Velocity ◽  
Luminal Flow ◽  
Red Cell Velocity

The response of third-order arterioles (n = 15) in rat cremaster muscle to increased luminal flow was studied after brief (20-30 s) occlusion of a neighboring arteriole in pentobarbital-anesthetized rats. Red cell velocity increased almost fivefold (485 +/- 54% of control) during occlusion, and vessel diameter increased 63 +/- 11%. Initially, the calculated wall shear rate increased to 430 +/- 40% of control during occlusion but then decreased to 308 +/- 35% of control as a consequence of arteriolar dilation. The muscle was subsequently stimulated to contract for 1 min, and the occlusion procedure was repeated after arteriolar diameter and red cell velocity had returned to control levels. In this instance the vessel dilation was 34 +/- 10% or about one-half of that seen during the previous occlusion, although velocity and shear rate rose to a similar degree (474 +/- 54 and 397 +/- 35%, respectively). Dilation during a third occlusion 2-7 min after the vessel recovered from the second occlusion was as great as during the first occlusion (77 +/- 20%). The results indicate that flow-induced dilation in arterioles of rat cremaster muscle is transiently attenuated after muscle contraction.

Capillary network geometry and red cell distribution in hamster cremaster muscle

1982 ◽  
Vol 242 (2) ◽  
pp. H211-H219 ◽  
Author(s):  
B. Klitzman ◽  
P. C. Johnson
Keyword(s):  
Blood Cells ◽  
Red Cell ◽  
Cell Distribution ◽  
Oxygen Level ◽  
Cremaster Muscle ◽  
Network Geometry ◽  
Cell Velocity ◽  
Flow Length ◽  
Red Cell Velocity ◽  
Vascular Geometry

Vascular geometry and red cell distribution were examined in 133 capillaries by means of intravital microscopy in the cremaster muscle of the pentobarbital-anesthetized hamster. Significant correlations (P less than 0.01) were found between diameter and flow, length and resistance, red cell transit time and flow, and red cell flux and hematocrit. Most unexpected was the lack of correlation (P greater than 0.2) between flow and resistance, length, or hematocrit. In addition, we analyzed the relation between blood flow and red cell flux at capillary bifurcations. The red blood cells had a slight (7%), but significant (P less than 0.025), tendency to enter the branch having either higher flow, red cell velocity, or pseudoshear rate (velocity/diameter). The net effect of the preferential red cell flow was to reduce mean capillary hematocrit from 13.7% prior to the bifurcation to 13.3% in the two branches. Finally, increasing the oxygen level of the superfusate above the muscle caused vasoconstriction and an increased heterogeneity of capillary red cell flux and hematocrit, decreasing the capacity for oxygen transport to the tissue.

RED-CELL SURFACE CHARGE IN GLOMERULAR DISEASE

The Lancet ◽  
1986 ◽  
Vol 328 (8507) ◽  
pp. 635-636 ◽  
Author(s):  
J. Feehally ◽  
A. Samanta ◽  
H. Kinghorn ◽  
A.C. Burden ◽  
J. Walls ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Glomerular Disease ◽  
Red Cell ◽  
Cell Surface Charge

RED-CELL SURFACE CHARGE IN PATIENTS WITH NEPHROTIC SYNDROME

The Lancet ◽  
1988 ◽  
Vol 331 (8600) ◽  
pp. 1459 ◽  
Author(s):  
H.T. Cohen ◽  
A.K. Singh ◽  
B.S. Kasinath ◽  
E.J. Lewis
Keyword(s):  
Nephrotic Syndrome ◽  
Cell Surface ◽  
Surface Charge ◽  
Red Cell ◽  
Cell Surface Charge

Effects of NO-Donors on Thrombus Formation and Microcirculation in Cerebral Vessels of the Rat

1996 ◽  
Vol 76 (01) ◽  
pp. 111-117 ◽  
Author(s):  
Yasuto Sasaki ◽  
Junji Seki ◽  
John C Giddings ◽  
Junichiro Yamamoto
Keyword(s):  
Blood Flow ◽  
Thrombus Formation ◽  
Dependent Manner ◽  
Red Cell ◽  
Cell Velocity ◽  
Red Cell Velocity ◽  
The Mean ◽  
Wall Shear ◽  
Dose Dependent

SummarySodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1), are known to liberate nitric oxide (NO). In this study the effects of SNP and SIN-1 on thrombus formation in rat cerebral arterioles and venules in vivo were assessed using a helium-neon (He-Ne) laser. SNP infused at doses from 10 Μg/kg/h significantly inhibited thrombus formation in a dose dependent manner. This inhibition of thrombus formation was suppressed by methylene blue. SIN-1 at a dose of 100 Μg/kg/h also demonstrated a significant antithrombotic effect. Moreover, treatment with SNP increased vessel diameter in a dose dependent manner and enhanced the mean red cell velocity measured with a fiber-optic laser-Doppler anemometer microscope (FLDAM). Blood flow, calculated from the mean red cell velocity and vessel diameters was increased significantly during infusion. In contrast, mean wall shear rates in the arterioles and venules were not changed by SNP infusion. The results indicated that SNP and SIN-1 possessed potent antithrombotic activities, whilst SNP increased cerebral blood flow without changing wall shear rate. The findings suggest that the NO released by SNP and SIN-1 may be beneficial for the treatment and protection of cerebral infarction

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

Bio-fabricated silver nanoparticles preferentially targets Gram positive depending on cell surface charge

2016 ◽  
Vol 83 ◽  
pp. 548-558 ◽  
Author(s):  
Debasis Mandal ◽  
Sandeep Kumar Dash ◽  
Balaram Das ◽  
Sourav Chattopadhyay ◽  
Totan Ghosh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Surface ◽  
Surface Charge ◽  
Gram Positive ◽  
Cell Surface Charge
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