Influence of red cell surface charge on red cell membrane curvature

1988 ◽  
Vol 413 (1) ◽  
pp. 77-82 ◽  
Author(s):  
R. Grebe ◽  
H. Wolff ◽  
H. Schmid-Sch�nbein
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Surface Charge ◽  
Membrane Curvature ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Cell Surface Charge

roles of carrier proteins. The identification and usefulness of blood group antigens as markers will be described and possible explanations for their variation in expression will be discussed. Most red cell antigens have been investigated because they are clinically important [1]. The antibodies to some antigens have caused haemolytic disease of the newborn and/or transfusion reactions. Other antigens are involved in haemolytic anaemia and some are important in transplantation. Red cell antigens provided a tool for investigation of the red cell surface and for use as genetic, immunological and biochemical markers. More than 500 red cell antigens are serologically defined, about half of which have been officially recognised and have been numbered by the International Society of Blood Transfusion Working Party on Terminology for Red Cell Surface Antigens [2,3]. Antigens are divided into systems (antigens controlled by a locus or closely linked loci) and three holding files: collections (related antigens whose genetic relationship is unknown), antigens of high incidence or antigens of low incidence. THE MAIEA TECHNIQUE Sometimes if an antigen has a very high or a very low incidence it is hard to relate it to other antigens or to assign it to a system. Immunochemical studies and in the case of high incidence antigens, use of cells of rare phenotype can be informative and recently the MAIEA technique, monoclonal antibody specific immobilisation of erythrocyte antigens, has proved useful. MAIEA was an adaptation of a technique, MAIPA, frequently used for studying platelets. MAIEA can be used to assign red cells antigens, as recognised by human alloantisera, to particular components of the red cell membrane [4]. Location of antigens on specific red cell membrane components The Knops system consists of 4 high incidence antigens Kna, McCa, Sla and Yka with frequencies greater than 90% in populations tested. There is also a low incidence antigen Knb found in Whites [3]. The antibodies to these public antigens are difficult to identify serologically. The antigens show a wide variation of strength on different donor’s cells. There is a null phenotype, the Helgeson phenotype, which appears from serological tests to lack all 4 antigens [5]. Cells which lack one Knops antigen may have weakened expression of other Knops antigens. The mists about these serologically difficult antigens were cleared when Moulds et al [6] and Rao et al [7] independently showed that these antigens were carried on the CR1 (complement receptor 1, CD35) protein. The related antigen Csa was not located on CR1, so Csa and Csb were left in the Cost collection [3].

1995 ◽  
pp. 188-188
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Working Party ◽  
Red Cell ◽  
Cell Surface Antigens ◽  
Serological Tests ◽  
Red Cell Membrane ◽  
Red Cell Antigens ◽  
High Incidence ◽  
Low Incidence

RED-CELL SURFACE CHARGE IN GLOMERULAR DISEASE

The Lancet ◽  
1986 ◽  
Vol 328 (8507) ◽  
pp. 635-636 ◽  
Author(s):  
J. Feehally ◽  
A. Samanta ◽  
H. Kinghorn ◽  
A.C. Burden ◽  
J. Walls ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Glomerular Disease ◽  
Red Cell ◽  
Cell Surface Charge

RED-CELL SURFACE CHARGE IN PATIENTS WITH NEPHROTIC SYNDROME

The Lancet ◽  
1988 ◽  
Vol 331 (8600) ◽  
pp. 1459 ◽  
Author(s):  
H.T. Cohen ◽  
A.K. Singh ◽  
B.S. Kasinath ◽  
E.J. Lewis
Keyword(s):  
Nephrotic Syndrome ◽  
Cell Surface ◽  
Surface Charge ◽  
Red Cell ◽  
Cell Surface Charge

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

Bio-fabricated silver nanoparticles preferentially targets Gram positive depending on cell surface charge

2016 ◽  
Vol 83 ◽  
pp. 548-558 ◽  
Author(s):  
Debasis Mandal ◽  
Sandeep Kumar Dash ◽  
Balaram Das ◽  
Sourav Chattopadhyay ◽  
Totan Ghosh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Surface ◽  
Surface Charge ◽  
Gram Positive ◽  
Cell Surface Charge
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