scholarly journals Simultaneous expression of cardiac and skeletal muscle isoforms of the L-type Ca2+ channel in a rat heart muscle cell line.

1994 ◽  
Vol 478 (2) ◽  
pp. 315-329 ◽  
Author(s):  
R Mejía-Alvarez ◽  
G F Tomaselli ◽  
E Marban
Keyword(s):  
Skeletal Muscle ◽  
Cell Line ◽  
Muscle Cell ◽  
Heart Muscle ◽  
Rat Heart ◽  
Heart Muscle Cell ◽  
Simultaneous Expression ◽  
Muscle Cell Line ◽  
Muscle Isoforms

scholarly journals Stable transfection of fatty acid translocase (CD36) in a rat heart muscle cell line (H9c2)

1998 ◽  
Vol 39 (10) ◽  
pp. 2039-2047 ◽  
Author(s):  
Frans A. Van Nieuwenhoven ◽  
Joost J.F.P. Luiken ◽  
Yvonne F. De Jong ◽  
Paul A. Grimaldi ◽  
Ger J. Van der Vusse ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Muscle Cell ◽  
Heart Muscle ◽  
Rat Heart ◽  
Stable Transfection ◽  
Heart Muscle Cell ◽  
Fatty Acid Translocase ◽  
Muscle Cell Line

Activation by curare of acetylcholine receptor channels in a murine skeletal muscle cell line

1989 ◽  
Vol 67 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Catherine E. Morris ◽  
Madeleine R. Montpetit ◽  
Wade J. Sigurdson ◽  
Kuni Iwasa
Keyword(s):  
Skeletal Muscle ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Single Channels ◽  
Agonist Action ◽  
Murine Skeletal Muscle ◽  
Skeletal Muscle Cell Line ◽  
Muscle Cell Line

Curare action on nicotinic acetylcholine receptors has a number of facets, of which the best known is competitive antagonism. Here we describe the weak agonist action of 10−5 M curare on the murine skeletal muscle cell line, G8. Although curare induces no depolarization in G8 cells, single-channel recordings reveal short-lived curare-induced currents. A feature of these brief events is the multiplicity of conductance levels (of the four levels with conductances of 48, 37, 14, and 6 pS, none had a lifetime greater than 1.5 ms). Most well-resolved events (about 17% of which are to a subconductance) last less than 0.5 ms, with activation occurring predominantly as isolated events rather than in bursts. Agonism is not, however, a high probability action for curare: calculations based on the frequency of events at half-saturating conditions suggest that curare-induced channel openings occur during less than 1% of acetylcholine receptor – curare binding episodes. The outcome is (a) an agonist action too feeble to perturb the membrane voltage and (b) a powerful competitive antagonist action.Key words: curare, acetylcholine receptor, single channels, skeletal muscle.

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