scholarly journals Calcitonin gene-related peptide activated ATP-sensitive K+ currents in rabbit arterial smooth muscle via protein kinase A.

1994 ◽  
Vol 475 (1) ◽  
pp. 9-13 ◽  
Author(s):  
J M Quayle ◽  
A D Bonev ◽  
J E Brayden ◽  
M T Nelson
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Calcitonin Gene Related Peptide ◽  
Arterial Smooth Muscle ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
K Currents ◽  
Kinase A

Protein kinase A mediates activation of ATP-sensitive K+ currents by CGRP in gallbladder smooth muscle

1994 ◽  
Vol 267 (3) ◽  
pp. G494-G499 ◽  
Author(s):  
L. Zhang ◽  
A. D. Bonev ◽  
G. M. Mawe ◽  
M. T. Nelson
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Whole Cell Patch Clamp ◽  
Camp Analogue ◽  
Calcitonin Gene ◽  
Transduction Mechanisms ◽  
Spontaneous Action ◽  
Spontaneous Action Potentials ◽  
Kinase A

The signal transduction mechanisms underlying the activation of ATP-sensitive potassium (KATP) current by calcitonin gene-related peptide (CGRP) in gallbladder smooth muscle were examined with intracellular microelectrode recording and whole cell patch-clamp techniques. In the intact gallbladder preparation, the adenylyl cyclase activator forskolin hyperpolarized the membrane potential and abolished spontaneous action potentials. This response was inhibited by the KATP channel blocker glibenclamide. CGRP (10 nM), forskolin (10 microM), the membrane-permeable adenosine 3',5'-cyclic monophosphate (cAMP) analogue adenosine 3',5'-cyclic monophosphothioate (Sp-cAMP[S]; 500 microM), and the catalytic subunit of protein kinase A (100 U/ml) activated glibenclamide-sensitive currents in enzymatically dissociated gallbladder smooth muscle cells. CGRP activation of potassium currents was prevented by dialysis of the cell cytoplasm with guanosine 5'-O-(2-thiodiphosphate) (5 mM) or a specific peptide inhibitor of protein kinase A (2.3 microM). Okadaic acid (5 microM), a phosphatase inhibitor, slowed the deactivation of the KATP current, following removal of CGRP. The results of this study indicate that CGRP hyperpolarizes gallbladder smooth muscle by elevation of cAMP and subsequent stimulation of protein kinase A.

Adenosine A1 Receptor-Mediated Inhibition of Protein Kinase A-Induced Calcitonin Gene-Related Peptide Release from Rat Trigeminal Neurons

2001 ◽  
Vol 59 (6) ◽  
pp. 1533-1541 ◽  
Author(s):  
Alan M. Carruthers ◽  
Lynda A. Sellers ◽  
David W. Jenkins ◽  
Emma M. Jarvie ◽  
Wasyl Feniuk ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Adenosine A1 Receptor ◽  
Related Peptide ◽  
Trigeminal Neurons ◽  
Calcitonin Gene ◽  
Peptide Release ◽  
Adenosine A1 ◽  
A1 Receptor ◽  
Kinase A
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