scholarly journals Cyclic AMP mediates inhibition of the Na(+)-K+ electrogenic pump by serotonin in tactile sensory neurones of the leech.

1993 ◽  
Vol 462 (1) ◽  
pp. 229-242 ◽  
Author(s):  
S Catarsi ◽  
R Scuri ◽  
M Brunelli
Keyword(s):  
Cyclic Amp ◽  
Electrogenic Pump ◽  
Sensory Neurones

Capsaicin-Induced Ion Fluxes Increase Cyclic GMP but Not Cyclic AMP Levels in Rat Sensory Neurones in Culture

1989 ◽  
Vol 53 (4) ◽  
pp. 1203-1211 ◽  
Author(s):  
J. N. Wood ◽  
P. R. Coote ◽  
A. Minhas ◽  
I. Mullaney ◽  
M. McNeill ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Cyclic Gmp ◽  
Ion Fluxes ◽  
Sensory Neurones

scholarly journals Modulation of the serotonin-sensitive potassium channel in Aplysia sensory neurone cell body and growth cone

1986 ◽  
Vol 124 (1) ◽  
pp. 287-306
Author(s):  
S. A. Siegelbaum ◽  
F. Belardetti ◽  
J. S. Camardo ◽  
M. J. Shuster
Keyword(s):  
Cyclic Amp ◽  
Potassium Channel ◽  
Direct Evidence ◽  
Single Channel ◽  
Growth Cones ◽  
Single Channel Recording ◽  
Sensory Neurone ◽  
Sensory Neurones ◽  
Channel Modulation ◽  
Insight Into

Using single-channel recording, we have been able to obtain some insight into the molecular mechanism of a modulatory transmitter action in Aplysia sensory neurones. Our results show that serotonin produces a slow EPSP and increases action potential duration in the sensory neurones by producing prolonged closures of the S potassium channel. Such closures appear to be mediated by cyclic AMP-dependent phosphorylation of a membrane protein which may be the channel. Modulation of S channels by serotonin also occurs in sensory neurone growth cones. This provides the first direct evidence that channel modulation occurs in nerve processes and increases the likelihood of channel modulation at the nerve terminal.

scholarly journals Serotonin depresses the after-hyperpolarization through the inhibition of the Na+/K+ electrogenic pump in T sensory neurones of the leech

1991 ◽  
Vol 155 (1) ◽  
pp. 261-273
Author(s):  
S. Catarsi ◽  
M. Brunelli
Keyword(s):  
T Cells ◽  
Electrical Stimulation ◽  
Mechanism Of Action ◽  
Room Temperature ◽  
Saline Solution ◽  
Electrogenic Pump ◽  
Sensory Neurones

In T sensory neurones of the leech, a train of impulses elicited by intracellular electrical stimulation leads to an after-hyperpolarization of up to 30 mV, mainly due to the activation of the electrogenic Na+/K(+)-ATPase but partly to a Ca2(+)-activated K+ conductance. It was found that serotonin reversibly reduced the amplitude of this after-hyperpolarization. We investigated the mechanism of action of serotonin and found: (1) after inhibition of the Ca2(+)-activated K+ conductance with BaCl2 or CdCl2, serotonin was still able to reduce the after-hyperpolarization; (2) when penetration of T cells with microelectrodes leaking sodium was preceded by serotonin perfusion of the ganglia, the normal hyperpolarization due to the activation of the electrogenic pump was converted to a depolarization; (3) after long-lasting perfusion with K(+)-free saline solution (which inhibits the Na+/K+ pump), the application of CsCl caused repolarization by reactivating the electrogenic ATPase; serotonin slowed and reduced this repolarization; (4) serotonin potentiated the depolarization of T neurones caused by the inhibition of the Na+/K+ pump following cooling of ganglia and depressed the hyperpolarization after rewarming to room temperature. These data taken together suggest that serotonin directly inhibits the Na+/K+ electrogenic pump.

Serotonin and cyclic AMP close single K+ channels in Aplysia sensory neurones

Nature ◽  
1982 ◽  
Vol 299 (5882) ◽  
pp. 413-417 ◽  
Author(s):  
Steven A. Siegelbaum ◽  
Joseph S. Camardo ◽  
Eric R. Kandel
Keyword(s):  
Cyclic Amp ◽  
Sensory Neurones ◽  
K Channels

Cyclic AMP-dependent protein kinase closes the serotonin-sensitive K+channels of Aplysia sensory neurones in cell-free membrane patches

Nature ◽  
1985 ◽  
Vol 313 (6001) ◽  
pp. 392-395 ◽  
Author(s):  
M. J. Shuster ◽  
J. S. Camardo ◽  
S. A. Siegelbaum ◽  
E. R. Kandel
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Dependent Protein Kinase ◽  
Sensory Neurones ◽  
K Channels ◽  
Dependent Protein ◽  
Free Membrane

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors

2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten
Keyword(s):  
Cyclic Amp ◽  
Adenosine Monophosphate ◽  
Adenylyl Cyclases ◽  
Temporal Precision ◽  
Fluorescent Biosensors ◽  
Subcellular Domains ◽  
Spatio Temporal ◽  
Hydrolysis Of ◽  
Shed Light ◽  
Cyclic Amp Signaling

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.

Decreased cyclic AMP in the epidermis of lesions of psoriasis

1972 ◽  
Vol 105 (5) ◽  
pp. 695-701 ◽  
Author(s):  
J. J. Voorhees
Keyword(s):  
Cyclic Amp
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