scholarly journals Neurosteroid regulation of GABAA receptor single-channel kinetic properties of mouse spinal cord neurons in culture.

1992 ◽  
Vol 456 (1) ◽  
pp. 215-245 ◽  
Author(s):  
R E Twyman ◽  
R L Macdonald
Keyword(s):  
Spinal Cord ◽  
Gabaa Receptor ◽  
Single Channel ◽  
Kinetic Properties ◽  
Spinal Cord Neurons ◽  
Mouse Spinal Cord ◽  
Channel Kinetic

Effect of cytoplasmic chloride concentration on open-state preference of glycine-activated chloride channels in cultured spinal cord cells

1993 ◽  
Vol 69 (3) ◽  
pp. 860-867 ◽  
Author(s):  
A. I. McNiven ◽  
A. R. Martin
Keyword(s):  
Spinal Cord ◽  
Chloride Channels ◽  
Single Channel ◽  
Chloride Concentration ◽  
Constant Field ◽  
Spinal Cord Neurons ◽  
Methane Sulphonate ◽  
State Preference ◽  
Lower Permeability ◽  
Open States

1. Glycine was bath-applied to outside-out patches taken from cultured mouse spinal cord neurons. Glycine-activated chloride channels had at least six open states with permeabilities of 40 (A), 68 (B), 100 (C), 145 (D), 180 (E), and 250 (F) al/s (1 attoliter = 10(-18) liter). Usually no more than two such states were seen in any given patch. The usual extracellular chloride concentration was 157 mM; chloride concentration in the electrode (on the cytoplasmic face of the patch) was varied between 7 and 170 mM, replaced at lower concentrations with either of the inert anions gluconate or methane sulphonate. 2. With normal (7 mM) chloride concentration in the electrode, openings to state D were the most probable, and the average permeability of all openings was 115 al/s. When the cytoplasmic chloride concentration was 20 mM, the most probable open state was the lower-permeability state (C), and the average permeability was 92 al/s. In symmetrical 170 mM chloride, the most probable open state was B and the mean permeability 77 al/s. 3. The glycine-activated channels had a vanishingly small permeability to fluoride. However, in experiments in which 150 mM gluconate in the pipette was replaced by fluoride, the most probable open state was B, as in the high-chloride solution. Thus the two halides had similar effects on open state preference. 4. With external and internal chloride concentrations of 7 and 157 mM, respectively, single-channel current-voltage relations could be fit with constant field relations appropriate to one or more of the permeability states.(ABSTRACT TRUNCATED AT 250 WORDS)

Spontaneous and GABA-induced single channel currents in cultured murine spinal cord neurons

1985 ◽  
Vol 63 (10) ◽  
pp. 1228-1233 ◽  
Author(s):  
David A. Mathers
Keyword(s):  
Spinal Cord ◽  
Time Constant ◽  
Single Channel ◽  
Resting Potential ◽  
Embryonic Mouse ◽  
Spinal Cord Neurons ◽  
Frequency Distributions ◽  
Exponential Term ◽  
Single Channel Currents ◽  
Channel Currents

Intracellular and patch clamp recordings were made from embryonic mouse spinal cord neurons growing in primary cell culture. Outside-out membrane patches obtained from these cells usually showed spontaneous single channel currents when studied at the resting potential (−56 ± 1.5 mV). In 18 out of 30 patches tested, spontaneous single channel activity was abolished by making Tris+ the major cation on both sides of the membrane. The remaining patches continued to display spontaneous single channel currents under these conditions. These events reversed polarity at a patch potential of 0 mV and displayed a mean single channel conductance of 24 ± 1.2 pS. Application of the putative inhibitory transmitter γ-aminobutyric acid (0.5–10 μM) to outside-out patches of spinal cord cell membrane induced single channel currents in 10 out of 15 patches tested. These channels had a primary conductance of 29 ± 2.8 pS in symmetrical 145 mM Cl solutions. Frequency distributions for the open times of these channels were well fit by the sum of a fast exponential term ("of") with a time constant τof = 4 ± 1.3 ms and a slow exponential term ("os") with a time constant τos = 24 ± 8.1 ms. Frequency distributions for channel closed times were also well fit by a double exponential equation, with time constants τcf = 2 ± 0.2 ms and τcs = 62 ± 20.9 ms.

scholarly journals Calcium-dependent action potentials in mouse spinal cord neurons in cell culture

1981 ◽  
Vol 220 (2) ◽  
pp. 408-415 ◽  
Author(s):  
Eric J. Heyer ◽  
Robert L. Macdonald ◽  
Gregory K. Bergey ◽  
Phillip G. Nelson
Keyword(s):  
Spinal Cord ◽  
Cell Culture ◽  
Action Potentials ◽  
Spinal Cord Neurons ◽  
Mouse Spinal Cord ◽  
Calcium Dependent
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