scholarly journals Unitary recordings of near threshold responses of receptor cells in the olfactory mucosa of the frog.

1978 ◽  
Vol 277 (1) ◽  
pp. 423-435 ◽  
Author(s):  
W Drongelen
Keyword(s):  
Olfactory Mucosa ◽  
Receptor Cells ◽  
Near Threshold

scholarly journals Histomorphological and microanatomical characteristics of the olfactory organ of freshwater carp, Cirrhinus reba (Hamilton)

2016 ◽  
Vol 24 (4) ◽  
pp. 201-208
Author(s):  
Saroj Kumar Ghosh ◽  
Padmanabha Chakrabarti
Keyword(s):  
Apical Part ◽  
Sensory Epithelium ◽  
Olfactory Mucosa ◽  
Cellular Organization ◽  
Mucous Cells ◽  
Functional Importance ◽  
Receptor Cells ◽  
Dorsal Portion ◽  
Olfactory Rosette ◽  
Scanning Electron

Abstract The morphoanatomy, cellular organization, and surface architecture of the olfactory apparatus in Cirrhinus reba (Hamilton) is described using light and scanning electron microscopy. The oval shaped olfactory rosette contained 32 ± 2 primary lamellae on each side of the median raphe, and was lodged on the floor of the olfactory chamber. The olfactory lamellae were basically flat and compactly arranged in the rosette. The olfactory chamber communicated to the outside aquatic environment through inlet and outlet apertures with a conspicuous nasal flap in between. The mid dorsal portion of the olfactory lamellae was characterized by a linguiform process. Sensory and non-sensory regions were distributed separately on each lamella. The sensory epithelium occupied the apical part including the linguiform process, whereas the resting part of the lamella was covered with non-sensory epithelium. The sensory epithelium comprised both ciliated and microvillous receptor cells distinguished by the architecture on their apical part. The non-sensory epithelium possessed mucous cells, labyrinth cells, and stratified epithelial cells with distinctive microridges. The functional importance of the different cells lining the olfactory mucosa was correlated with the ecological habits of the fish examined.

Immunohistochemical Analysis of the Olfactory Mucosa by Use of Antibodies to Brain Proteins and Cytokeratin

1989 ◽  
Vol 98 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Masuo Yamagishi ◽  
Satoshi Hasegawa ◽  
Yuichi Nakano ◽  
Sugata Takahashi ◽  
Toshihiko Iwanaga
Keyword(s):  
Lamina Propria ◽  
Neuron Specific Enolase ◽  
Immunohistochemical Analysis ◽  
Olfactory Mucosa ◽  
Basal Cells ◽  
Neurofilament Protein ◽  
Receptor Cells ◽  
Nerve Bundles ◽  
Protein Immunoreactivity ◽  
Cerebellar Purkinje Cells

The present study deals with the immunohistochemical detection of four brain-derived proteins and cytokeratin in the normal olfactory mucosa of humans and guinea pigs. Neurofilament protein immunoreactivity was found in the olfactory vesicles, dendrites, and perikaryon of receptor cells, and in thick nerve bundles located deep in the lamina propria. The antiserum to neuron-specific enolase (NSE) selectively stained olfactory receptor cells throughout the length of the bundles. The NSE immunoreactivity also was recognized in nerve bundles of various sizes throughout the lamina propria. Glia-specific S-100 protein immunoreactivity was present in Bowman's glands as well as in all nerve bundles in the lamina propria, but not in any cellular elements constituting the olfactory epithelium. Immunoreactivity for spot-35 protein, which was considered to be specific for cerebellar Purkinje cells, was found in flasklike cells (microvillar cells) occurring near the free surface of the epithelium. The basal cells in the olfactory and respiratory epithelium were stained positively with a cytokeratin antiserum.

Olfactory mucosa tissue-based biosensor: A bioelectronic nose with receptor cells in intact olfactory epithelium

2010 ◽  
Vol 146 (2) ◽  
pp. 527-533 ◽  
Author(s):  
Qingjun Liu ◽  
Weiwei Ye ◽  
Hui Yu ◽  
Ning Hu ◽  
Liping Du ◽  
...  
Keyword(s):  
Olfactory Epithelium ◽  
Olfactory Mucosa ◽  
Receptor Cells

scholarly journals The Olfactory Mucosa of the Sheep

1970 ◽  
Vol 23 (2) ◽  
pp. 447 ◽  
Author(s):  
Jean E Kratzing
Keyword(s):  
Electron Microscopy ◽  
Lamina Propria ◽  
Light And Electron Microscopy ◽  
Free Edge ◽  
Cell Types ◽  
Olfactory Nerve ◽  
Olfactory Mucosa ◽  
Basal Cells ◽  
Supporting Cells ◽  
Receptor Cells

The olfactory mucosa of the sheep was studied by light and electron microscopy. The epithelium conforms to the general vertebrate pattern and consists of olfactory receptor cells, supporting, and basal cells. The free edge of the epithelium is made up of long microvilli from the supporting cells and olfactory rods of the receptor cells, each carrying 40-50 cilia. All cell types contain large dark granules which may be the site of olfactory pigment. The basement membrane is not visible in light microscopy and is fine and discontinuous in electron microscopy. Bowman's glands are simple, tubular, mucus-secreting glands in the lamina propria. Their cells contain basal granules resembling those in the epithelial cells. The lamina propria also contains bundles of fine, unmyelinated, olfactory nerve fibres which are the proximal continuations of the receptor cells.

Olfactory Mucosa of Patients with Olfactory Disturbance following Head Trauma

1994 ◽  
Vol 103 (4) ◽  
pp. 279-284 ◽  
Author(s):  
Masuo Yamagishi ◽  
Ryusuke Okazoe ◽  
Yoichi Ishizuka
Keyword(s):  
Head Trauma ◽  
Olfactory Receptor ◽  
Neuron Specific Enolase ◽  
Protein S ◽  
Olfactory Mucosa ◽  
Olfactory Receptor Cells ◽  
Receptor Cells ◽  
Nerve Bundles ◽  
S 100 ◽  
Olfactory Disturbance

The olfactory mucosa in 7 patients with olfactory disturbance following head trauma were sampled for biopsy with special biopsy forceps and examined by immunohistochemical staining with anti—neuron-specific enolase (NSE) and S-100 protein (S-100) antibodies. The residual olfactory receptor cells and nerve bundles were counted, and the degree of degeneration was determined. In 5 patients, olfactory receptor cells that reacted with anti-NSE antiserum remained, although the number varied with the patient, and in 2 patients the receptor cells disappeared. In the lamina propria, the S-100–immunoreactive olfactory nerves were retained in 6 patients. The outcome was poor in all cases regardless of the number of residual receptor cells and nerve bundles. These results indicate that the degree of impairment of the peripheral olfactory region after head trauma differs from case to case, and that even if the receptor cells and nerve bundles remain, it is difficult to improve the condition, although some cases of malingering may be contained.

Receptor cells of the catfish olfactory mucosa

1983 ◽  
Vol 8 (2) ◽  
pp. 203-209 ◽  
Author(s):  
P. Cancalon
Keyword(s):  
Olfactory Mucosa ◽  
Receptor Cells

Cytoplasmic Organization in the Receptor Cells of the Crista Ampullaris

1972 ◽  
Vol 30 ◽  
pp. 60-61 ◽  
Author(s):  
Robert F. Dunn
Keyword(s):  
Fine Particles ◽  
Apical Cell ◽  
Apical Region ◽  
Receptor Cells ◽  
Cuticular Plate ◽  
Morphological Organization ◽  
Crista Ampullaris ◽  
Cytoplasmic Organization ◽  
High Degree ◽  
General Appearance

Receptor cells of the cristae in the vestibular labyrinth of the bullfrog, Rana catesbiana, show a high degree of morphological organization. Four specialized regions may be distinguished: the apical region, the supranuclear region, the paranuclear region, and the basilar region.The apical region includes a single kinocilium, approximately 40 stereocilia, and many small microvilli all projecting from the apical cell surface into the lumen of the ampulla. A cuticular plate, located at the base of the stereocilia, contains filamentous attachments of the stereocilia, and has the general appearance of a homogeneous aggregation of fine particles (Fig. 1). An accumulation of mitochondria is located within the cytoplasm basal to the cuticular plate.

Ultrastructural aspects of olfactory signal transduction and its development

1994 ◽  
Vol 52 ◽  
pp. 142-143
Author(s):  
Bert Ph. M. Menco
Keyword(s):  
Signal Transduction ◽  
Olfactory Receptor ◽  
Receptor Cell ◽  
Freeze Substitution ◽  
Luminal Surface ◽  
Tissue Sections ◽  
Olfactory Receptor Cells ◽  
Receptor Cells ◽  
Olfactory Signal ◽  
Odor Molecule

Vertebrate olfactory receptor cells are specialized neurons that have numerous long tapering cilia. The distal parts of these cilia line the interface between the external odorous environment and the luminal surface of the olfactory epithelium. The length and number of these cilia results in a large surface area that presumably increases the chance that an odor molecule will meet a receptor cell. Advanced methods of cryoprepration and immuno-gold labeling were particularly useful to preserve the delicate ultrastructural and immunocytochemical features of olfactory cilia required for localization of molecules involved in olfactory signal-transduction. We subjected olfactory tissues to freeze-substitution in acetone (unfixed tissues) or methanol (fixed tissues) followed by low temperature embedding in Lowicryl K11M for that purpose. Tissue sections were immunoreacted with several antibodies against proteins that are presumably important in olfactory signal-transduction.

Hearing in Mice via GSR Audiometry

1963 ◽  
Vol 6 (4) ◽  
pp. 359-368 ◽  
Author(s):  
Charles I. Berlin
Keyword(s):  
Inverse Relationship ◽  
Sound Pressure ◽  
Single Units ◽  
Sensitivity Curve ◽  
Basic Tool ◽  
Eighth Nerve ◽  
Pure Tones ◽  
Conditioned Responses ◽  
Frequency Intensity ◽  
Near Threshold

Hearing in mice has been difficult to measure behaviorally. With GSR as the basic tool, the sensitivity curve to pure tones in mice has been successfully outlined. The most sensitive frequency-intensity combination was 15 000 cps at 0-5 dB re: 0.0002 dyne/cm 2 , with responses noted from 1 000 to beyond 70 000 cps. Some problems of reliability of conditioning were encountered, as well as findings concerning the inverse relationship between the size of GSR to unattenuated tones and the sound pressure necessary to elicit conditioned responses at or near threshold. These data agree well with the sensitivity of single units of the eighth nerve of the mouse.

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