scholarly journals The Olfactory Mucosa of the Sheep

1970 ◽  
Vol 23 (2) ◽  
pp. 447 ◽  
Author(s):  
Jean E Kratzing
Keyword(s):  
Electron Microscopy ◽  
Lamina Propria ◽  
Light And Electron Microscopy ◽  
Free Edge ◽  
Cell Types ◽  
Olfactory Nerve ◽  
Olfactory Mucosa ◽  
Basal Cells ◽  
Supporting Cells ◽  
Receptor Cells

The olfactory mucosa of the sheep was studied by light and electron microscopy. The epithelium conforms to the general vertebrate pattern and consists of olfactory receptor cells, supporting, and basal cells. The free edge of the epithelium is made up of long microvilli from the supporting cells and olfactory rods of the receptor cells, each carrying 40-50 cilia. All cell types contain large dark granules which may be the site of olfactory pigment. The basement membrane is not visible in light microscopy and is fine and discontinuous in electron microscopy. Bowman's glands are simple, tubular, mucus-secreting glands in the lamina propria. Their cells contain basal granules resembling those in the epithelial cells. The lamina propria also contains bundles of fine, unmyelinated, olfactory nerve fibres which are the proximal continuations of the receptor cells.

Fine Structure of the Eye of a Nudibranch Mollusc, Hermissenda Crassicornis

1967 ◽  
Vol 2 (3) ◽  
pp. 349-358
Author(s):  
R. M. EAKIN ◽  
JANE A. WESTFALL ◽  
M. J. DENNIS
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Optic Nerve ◽  
Light And Electron Microscopy ◽  
The Other ◽  
Sensory Cells ◽  
Nerve Fibres ◽  
Supporting Cells ◽  
Small Bodies ◽  
Receptor Cells

The eye of a nudibranch, Hermissenda crassicornis, was studied by light and electron microscopy. Three kinds of cells were observed: large sensory cells, each bearing at one end an array of microvilli (rhabdomere) and at the other end an axon which leaves the eye by the optic nerve; large pigmented supporting cells; and small epithelial cells, mostly corneal. There are five sensory cells, and the same number of nerve fibres in the optic nerve. The receptor cells contain an abundance of small vesicles, 600-800 Å in diameter. The lens is a spheroidal mass of osmiophilic, finely granular material. A basal lamina and a capsule of connective tissue enclose the eye. In some animals the eye is ‘infected’ with very small bodies, 4-5 µ in diameter, thought to be symbionts.

Immunohistochemical Analysis of the Olfactory Mucosa by Use of Antibodies to Brain Proteins and Cytokeratin

1989 ◽  
Vol 98 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Masuo Yamagishi ◽  
Satoshi Hasegawa ◽  
Yuichi Nakano ◽  
Sugata Takahashi ◽  
Toshihiko Iwanaga
Keyword(s):  
Lamina Propria ◽  
Neuron Specific Enolase ◽  
Immunohistochemical Analysis ◽  
Olfactory Mucosa ◽  
Basal Cells ◽  
Neurofilament Protein ◽  
Receptor Cells ◽  
Nerve Bundles ◽  
Protein Immunoreactivity ◽  
Cerebellar Purkinje Cells

The present study deals with the immunohistochemical detection of four brain-derived proteins and cytokeratin in the normal olfactory mucosa of humans and guinea pigs. Neurofilament protein immunoreactivity was found in the olfactory vesicles, dendrites, and perikaryon of receptor cells, and in thick nerve bundles located deep in the lamina propria. The antiserum to neuron-specific enolase (NSE) selectively stained olfactory receptor cells throughout the length of the bundles. The NSE immunoreactivity also was recognized in nerve bundles of various sizes throughout the lamina propria. Glia-specific S-100 protein immunoreactivity was present in Bowman's glands as well as in all nerve bundles in the lamina propria, but not in any cellular elements constituting the olfactory epithelium. Immunoreactivity for spot-35 protein, which was considered to be specific for cerebellar Purkinje cells, was found in flasklike cells (microvillar cells) occurring near the free surface of the epithelium. The basal cells in the olfactory and respiratory epithelium were stained positively with a cytokeratin antiserum.

Immunocytochemical localization of S-100 beta beta protein in olfactory and supporting cells of lamb olfactory epithelium.

1989 ◽  
Vol 37 (12) ◽  
pp. 1825-1833 ◽  
Author(s):  
M G Rambotti ◽  
C Saccardi ◽  
A Spreca ◽  
M C Aisa ◽  
I Giambanco ◽  
...  
Keyword(s):  
Schwann Cells ◽  
Olfactory Epithelium ◽  
Plasma Membranes ◽  
Immune Reaction ◽  
Protein S ◽  
Cell Types ◽  
Olfactory Nerve ◽  
Basal Cells ◽  
Supporting Cells ◽  
S 100

By immunocytochemistry, we have identified two novel cell types, olfactory and supporting cells of lamb olfactory epithelium, expressing S-100 beta beta protein. S-100 immune reaction product was observed on ciliary and plasma membranes, on axonemes and in the cytoplasm adjacent to plasma membranes and to basal bodies of olfactory vesicles. A brief treatment of olfactory mucosae with Triton X-100 before fixation is necessary for detection of S-100 beta beta protein within olfactory vesicles. In the absence of such a treatment, the immune reaction product is restricted to ciliary and plasma membranes. On the other hand, irrespective of pre-treatment of olfactory mucosae, S-100 beta immune reaction product in supporting cells is restricted to microvillar and plasma membranes. The anti-S-100 beta antiserum used in these studies does not bind to basal cells of the olfactory epithelium or to cells of the olfactory glands, whereas it binds to Schwann cells of the olfactory nerve. An anti-S-100 alpha antiserum does not bind to cellular elements of the olfactory mucosa, Schwann cells, or axons of the olfactory nerve. The present data provide, for the first time, evidence for the presence of S-100 beta beta protein in mammalian neurons (olfactory cells).

The structure of the gills of the axolotl, Ambystoma mexicanum

1987 ◽  
Vol 45 ◽  
pp. 824-825
Author(s):  
Mohinder S. Jarial
Keyword(s):  
Leydig Cells ◽  
Secretory Granules ◽  
Light And Electron Microscopy ◽  
Cell Types ◽  
Ambystoma Mexicanum ◽  
Basal Cells ◽  
Granular Cells ◽  
Gill Filaments ◽  
Mitotic Figures ◽  
Apical Membranes

The axolotl is a strictly aquatic salamander in which the larval external gills are retained throughout life. The external gills of the adult axolotl have been studied by light and electron microscopy for ultrastructural evidence of ionic transport. The thin epidermis of the gill filaments and gill stems is composed of 3 cell types: granular cells, the basal cells and a sparce population of intervening Leydig cells. The gill epidermis is devoid of muscles, and no mitotic figures were observed in any of its cells.The granular cells cover the gill surface as a continuous layer (Fig. 1, G) and contain secretory granules of different forms, located apically (Figs.1, 2, SG). Some granules are found intimately associated with the apical membrane while others fuse with it and release their contents onto the external surface (Fig. 3). The apical membranes of the granular cells exhibit microvilli which are covered by a PAS+ fuzzy coat, termed “glycocalyx” (Fig. 2, MV).

scholarly journals Histoanatomy and surface ultrastructure of the olfactory organ of the freshwater tank goby, Glossogobius giuris (Hamilton, 1822)

2020 ◽  
Vol 28 (3) ◽  
pp. 141-148
Author(s):  
Saroj Kumar Ghosh
Keyword(s):  
Nerve Fibers ◽  
Olfactory Organ ◽  
Basal Cells ◽  
Supporting Cells ◽  
Blood Capillaries ◽  
Surface Ultrastructure ◽  
Receptor Cells ◽  
Mucosal Lining ◽  
Characteristic Features ◽  
Glossogobius Giuris

AbstractCharacteristic features of histology and fine morphology of the olfactory organ in the tank goby, Glossogobius giuris (Perciformes, Gobiidae, Gobiinae), were investigated with light and scanning electron microscopy. The olfactory cavity contained single lamellae that were exposed to the aquatic environment by small anterior and posterior nostrils. Typical olfactory rosettes were not observed. Histologically, each lamella consisted of two layers of epithelium; wrapping the central core that was composed of connective tissue stroma with nerve fibers and blood capillaries. The mucosal lining of lamella was merged with sensory and non-sensory olfactory cells, identified on the basis of structural characters, surface specializations, and staining features. The principal sensory elements were ciliated receptor cells that were characterized by apical dendritic processes expanded from cell soma and microvillous receptor cells equipped with multiple tiny dendrons on the mucosal surface. The bead-like appearance of several labyrinth cells, mucous cells with secreted mucin, scattered lymphatic cells, stratified epithelial cells bearing microfolds, and condensed ciliated supporting cells were observed in the non-sensory epithelia. Undifferentiated basal cells were embedded in the deeper zone of the epithelium above the basement membrane. The cellular organization of the olfactory lining was interpreted with chemoreception of the fish concerned.

Probable mechanoreceptor structures of osphradia in marine Caenogastropoda

2020 ◽  
Vol 30 (1) ◽  
pp. 33-39
Author(s):  
N. N. Kamardin
Keyword(s):  
Electron Microscopy ◽  
Apical Surface ◽  
Supporting Cells ◽  
Receptor Cells ◽  
Single Receptor

TEM and SEM electron microscopy have been used to study osphradia in 6 species of marine Caenogastropoda. The ultrastructural features of mechanoreceptor cells that perform the Littorina osmoreception function in osphradium organs are presented. Mechanoreception is based on a possible change in the volume of cisterns of microvilli of supporting cells, which can be transmitted by the cilia of nearby mechanoreceptor cells. These cells obviously, have mechanosensory channels on the apical surface. It has been first discovered in predatory molluscs actively searching for food, that single receptor cells with a mobile sensilla consisting of several cilium were joined together. They are located along the groove zone and follow the direction and force of the movement of water along the osphradium petals.

scholarly journals Proximodistal degeneration of C-fibers detached from their perikarya.

1983 ◽  
Vol 97 (1) ◽  
pp. 6-14 ◽  
Author(s):  
P Cancalon
Keyword(s):  
Electron Microscopy ◽  
Cell Body ◽  
Constant Velocity ◽  
Light And Electron Microscopy ◽  
Olfactory Nerve ◽  
Slow Flow ◽  
Temperature Dependent ◽  
C Fibers ◽  
Cytoskeletal Elements

Degeneration was followed in the garfish olfactory nerve after removal of the mucosa containing the cell bodies. Degeneration, as measured by a decrease in the weight of consecutive 3-mm nerve segments, spreads at constant velocity from the site of injury toward the synaptic area. The proximodistal degeneration is temperature dependent and progresses from 0.3 mm/d at 10 degrees C to 13.0 mm/d at 35 degrees C. Between 14 and 35 degrees C, the velocity increases linearly with temperature. At all the temperatures investigated, these proximodistal degeneration velocities are identical to the rates of slow intraaxonal flow measured in axons detached from their cell bodies, or to the rates measured in regenerating fibers, and, except at 10 degrees C, are 3.3 times faster than the rate of slow flow in intact nerves. These results were confirmed by light and electron microscopy. We hypothesize that the collapse and subsequent degeneration of the axons is the result of a proximodistal depletion of cytoskeletal elements no longer provided by the cell body to the axon by slow intraaxonal flow. A significant number of axons disappeared rapidly from the nerve before the arrival of the slow degenerative wave. From studies by other groups, this rapid degeneration may be the result of a lack of rapidly transported, mainly membranous components.

scholarly journals The Fine Structure of the Wheat Scutellum During Germination

1972 ◽  
Vol 25 (3) ◽  
pp. 469 ◽  
Author(s):  
JG Swift ◽  
TP O'brien
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Light And Electron Microscopy ◽  
Cell Types ◽  
Wall Material ◽  
Cytological Evidence ◽  
Starch Grains ◽  
Parenchyma Cells ◽  
Cytological Changes

The cytological changes that take place in the scutellar epithelium and parenchyma during the first 5 days of germination are described by light and electron microscopy. Within 6 hr small starch grains appear in the plastids of both cell types and the size and number of starch grains increase gradually as germination proceeds. Later in germination starch disappears again from the plastids in the epithelial cells, but large starch grains still remain in the parenchyma cells. The reserves of the protein bodies are hydrolysed and the residual vacuoles undergo extensive coales-cence. Modifications in the appearance of the wall material of the epithelial cells as these cells elongate are illustrated and possible functional bases for these changes are suggested. The cells of the scutellar epithelium show no cytological evidence for their known functions of diastase secretion and nutrient absorption.

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