scholarly journals Biophysical and mechanical properties of red and white muscle fibres in fish

1969 ◽  
Vol 201 (1) ◽  
pp. 49-59 ◽  
Author(s):  
T. Hidaka ◽  
N. Toida
Keyword(s):  
Mechanical Properties ◽  
White Muscle ◽  
Muscle Fibres

The resting membrane potential of white muscle from brown trout (Salmo trutta) exposed to copper in soft, acidic water

2000 ◽  
Vol 203 (14) ◽  
pp. 2229-2236 ◽  
Author(s):  
M.W. Beaumont ◽  
E.W. Taylor ◽  
P.J. Butler
Keyword(s):  
Membrane Potential ◽  
Brown Trout ◽  
Salmo Trutta ◽  
White Muscle ◽  
Low Ph ◽  
Ammonium Ion ◽  
Muscle Membrane ◽  
Resting Membrane Potential ◽  
Muscle Fibres ◽  
Brown Trout Salmo Trutta

Previously, the distribution of ammonia between the intracellular and extracellular compartments has been used to predict a significant depolarisation of the resting membrane potential (E(M)) of white muscle from brown trout (Salmo trutta) exposed to a sub-lethal combination of copper and low pH. However, this prediction is based upon two assumptions (i) a relatively high membrane permeability for the ammonium ion with respect to that for ammonia gas and (ii) that this is unaltered by exposure to copper and low pH. Since there is conflicting evidence in the literature of the validity of these assumptions, in the present study E(M) was directly measured in white muscle fibres of trout exposed to copper and low pH (E(M)=−52.2+/−4.9 mV) and compared with that of unexposed, control animals (E(M)=−86.5+/−2.9 mV) (means +/− s.e.m., N=6). In confirming the predicted depolarisation, these data support the hypothesis of electrophysiological impairment as a factor in the reduction in the swimming performance of trout exposed to these pollutants. In addition, the results of this study support the role of a significant permeability of the muscle membrane to NH(4)(+) in determining the distribution of ammonia in fish.

Electrical and Mechanical Properties of the Red and White Muscles in the Silver Carp

1972 ◽  
Vol 57 (2) ◽  
pp. 551-567
Author(s):  
T. YAMAMOTO
Keyword(s):  
Mechanical Properties ◽  
White Muscle ◽  
Silver Carp ◽  
Membrane Resistance ◽  
Resting Potential ◽  
Resting Membrane Potential ◽  
Mechanical Responses ◽  
Red Muscle ◽  
Red And White Muscles ◽  
Specific Membrane

1. Electrical and mechanical properties of the red muscle (M. levator pinnae pectoralis) and white muscle (M. levator pinnae lateralis abdominis) in the silver carp (Carassius auratus Linné) were investigated by using caffeine and thymol. 2. A complete tetanus could be produced in the red muscle. But in the white muscle no tetanus was produced and there was a gradual decrease in tension during continuous stimulation, even at a frequency of 1 c/s or less. 3. Caffeine (0.5-1 mM) and thymol (0.25-0.5 mM) potentiated the twitch tension in both muscles without an increase in the resting tension; they produced a contracture in both muscles when the concentration was increased further. 4. The falling phase of the active state of contraction was nearly the same in both muscles and was prolonged by caffeine (0.5 mmM) and by thymol (0.25 mM). 5. The resting membrane potential of the red muscle was scarcely affected by caffeine (0.5-5 mM), whereas in the white muscles a depolarization of 10 mV was observed with caffeine of more than 2 mM. The resting potential of both muscles was little changed by o.25 mm thymol. However, at a concentration of more than 0.5mM thymol depolarized the membrane in both muscles to the same extent. 6. In caffeine (2-3 mM) solution the mean specific membrane resistance was reduced from 8.8 kΩ cm2 to 6.0 kΩ cm2 in the red muscle, and from 5.0 kΩ cm2 to 2.7 kΩ cm2 in the white muscle. In thymol (0.5-1 mM) solution it was reduced from 11.2 kΩcm2 to 6.5 kΩ cm2 in the red muscle, and from 5.4kΩ cm2 to 3.1 kΩ) cm2 in the white muscle. The specific membrane capacitance, calculated from the time constant and the membrane resistance, remained more or less the same in both muscles after a treatment with these agents. 7. Electrical properties of the muscles and the effects of caffeine and thymol on mechanical responses suggest that there are no fundamental differences between red and white muscles except for the excitation-contraction coupling. A lack of summation of twitch, a successive decline of twitch, and inability to produce potassium contracture in the white muscle may be due to the fact that the Ca-releasing mechanism is easily inactivated by depolarization of the membrane.

Early thermal experience has different effects on growth and muscle fibre recruitment in spring- and autumn-running Atlantic salmon populations

2000 ◽  
Vol 203 (17) ◽  
pp. 2553-2564 ◽  
Author(s):  
I.A. Johnston ◽  
H.A. McLay ◽  
M. Abercromby ◽  
D. Robins
Keyword(s):  
Atlantic Salmon ◽  
Satellite Cells ◽  
Temperature Regime ◽  
White Muscle ◽  
Growth Patterns ◽  
Muscle Fibres ◽  
Cross Sectional ◽  
Frequency Distributions ◽  
Muscle Satellite Cells ◽  
First Feeding

The consequence of early thermal experience for subsequent growth patterns was investigated in Atlantic salmon (Salmo salar L.). Spring- and autumn-running salmon were caught in upland (Baddoch) and lowland (Sheeoch) tributaries of the River Dee, Aberdeenshire, Scotland, respectively, on the final stages of their spawning migrations. The eggs were incubated at the simulated natural temperature regime of each stream, which was on average 2.8 degrees C lower for the Baddoch. The offspring, representing 11 families per population, were transferred at first feeding to constant environmental conditions (12–14 degrees C; 16h:8h light:dark photoperiod) and reared in replicate tanks. Salmon of both populations were longer and heavier at 6 and 12 weeks in fish initially reared under the cooler Baddoch regime. Length frequency distributions became bimodal after 18 weeks, and only the upper growth mode was studied. Modelling of length distributions at 40 weeks revealed significantly different patterns of muscle growth according to initial temperature regime, but only for the Sheeoch salmon. In fish of Sheeoch origin, significantly more white muscle fibres were recruited per mm(2) increase in myotomal cross-sectional area at Sheeoch than at Baddoch temperatures (P<0.01). After 40 weeks, the density of white fibres was 10.4 % higher in fish initially reared at the Sheeoch (533+/−6 mm(−2)) than at the Baddoch (483+/−5 mm(−2)) thermal regimes (means +/− s.e.m., 16 fish per group; P<0.001). Muscle satellite cells were identified using an antibody to c-met. At 24 weeks, the density of muscle satellite cells was 29 % higher in Sheeoch salmon reared to first feeding at the temperature of their natal stream than at cooler Baddoch temperatures (P<0.01). In contrast, the number and size distributions of white muscle fibres in the myotomes of Baddoch salmon were independent of early thermal experience.

The energetic cost of activation of white muscle fibres from the dogfish Scyliorhinus canicula

1997 ◽  
Vol 200 (3) ◽  
pp. 495-501 ◽  
Author(s):  
F Lou ◽  
N Curtin ◽  
R Woledge
Keyword(s):  
Heat Production ◽  
White Muscle ◽  
Isometric Force ◽  
Force Production ◽  
Total Heat ◽  
Energetic Cost ◽  
Maximum Efficiency ◽  
Muscle Fibres ◽  
Scyliorhinus Canicula ◽  
A Value

The energetic cost of activation was measured during an isometric tetanus of white muscle fibres from the dogfish Scyliorhinus canicula. The total heat production by the fibres was taken as a measure of the total energetic cost. This energy consists of two parts. One is due to crossbridge interaction which produces isometric force, and this part varies linearly with the degree of filament overlap in the fibres. The other part of the energy is that associated with activation of the crossbridges by Ca2+, mainly with uptake of Ca2+ into the sarcoplasmic reticulum by the ATP-driven Ca2+ pump. Total heat production was measured at various degrees of filament overlap beyond the optimum for force development. Extrapolation of heat versus force production data to evaluate the heat remaining at zero force gave a value of 34±5 % (mean ± s.e.m., N=24) for activation heat as a percentage of total heat production in a 2.0 s isometric tetanus. Values for 0.4 and 1.0 s of stimulation were similar. Comparison with values in the literature shows that the energetic cost of activation in dogfish muscle is very similar to that of frog skeletal muscle and it cannot explain the lower maximum efficiency of dogfish muscle compared with frog muscle. The proportion of energy for activation (Ca2+ turnover) is similar to that expected from a simple model in which Ca2+ turnover was varied to minimize the total energy cost for a contraction plus relaxation cycle.

White muscle strain in the common carp and red to white muscle gearing ratios in fish

1999 ◽  
Vol 202 (5) ◽  
pp. 521-528 ◽  
Author(s):  
J.M. Wakeling ◽  
I.A. Johnston
Keyword(s):  
Common Carp ◽  
High Speed ◽  
White Muscle ◽  
Current Data ◽  
Homogeneous Material ◽  
Muscle Fibres ◽  
Muscle Strain ◽  
The Common ◽  
Superficial Muscle ◽  
Helical Geometry

White muscle strains were recorded using sonomicrometry techniques for 70 fast-starts in the common carp Cyprinus carpio L. High-speed cine images were recorded simultaneously for 54 of these starts, and muscle strain was calculated independently from the digitized outlines of the fish. Sonomicrometry measurements of superficial muscle strain were not significantly different from the strain as calculated from the theory of simple bending of a homogeneous material: superficial muscle strain thus varied with chordwise distance from the spine. However, white muscle strain across a transverse section of the myotome shows less variation with chordwise position than would be expected from simple bending theory. Muscle strains measured using sonomicrometry thus do not necessarily represent the more uniform strain predicted for the whole section of the fish. White muscle strain can be accurately predicted from the spine curvatures as measured from the cine images if the gearing ratio between the red and white muscle fibres is known. A model for calculating the gearing ratio from the helical muscle fibre geometry was re-evaluated using current data for the kinematics of fast-starting C. carpio. This model predicted a mean gearing ratio of 2.8 for these fast-starts. A quicker, alternative approach to estimating gearing ratio from the position of the centroid of white fibre area is proposed and results in ratios similar to those calculated from the model of helical geometry. White muscle strains in fish can thus be estimated from measurements of spine curvature and muscle distribution alone.

scholarly journals Mechanics of myosin function in white muscle fibres of the dogfish, Scyliorhinus canicula

2012 ◽  
Vol 590 (8) ◽  
pp. 1973-1988 ◽  
Author(s):  
S. Park‐Holohan ◽  
M. Linari ◽  
M. Reconditi ◽  
L. Fusi ◽  
E. Brunello ◽  
...  
Keyword(s):  
White Muscle ◽  
Muscle Fibres ◽  
Scyliorhinus Canicula

scholarly journals Suxamethonium Fasciculations: A Topographical Study

1980 ◽  
Vol 8 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Clive B. Collier
Keyword(s):  
White Muscle ◽  
Pectoralis Major ◽  
Muscle Group ◽  
The Body ◽  
Muscle Fibres ◽  
Resistant Group

It has been found in a group of 455 gynaecological patients that not all the voluntary muscles of the body respond to suxamethonium in the same way. One group of muscles (e.g. biceps) which is sensitive to suxamethonium, responds by fasciculating in almost every individual. A second, resistant, group of muscles (e.g. pectoralis major) is almost totally unresponsive to suxamethonium. A third muscle group (e.g. triceps) shows fasciculations in about 60% of cases and appears intimately associated with the development of suxamethonium pains, for if fasciculations do occur then suxamethonium pains are likely to ensue, but not necessarily involving all the fasciculating muscles. A theory to account for these different groups has been proposed involving the known variation in distribution of red and white muscle fibres throughout the body.

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