scholarly journals Electrical pulse stimulation induces differential responses in insulin action in myotubes from severely obese individuals

2018 ◽  
Vol 597 (2) ◽  
pp. 449-466 ◽  
Author(s):  
Sanghee Park ◽  
Kristen D. Turner ◽  
Donghai Zheng ◽  
Jeffrey J. Brault ◽  
Kai Zou ◽  
...  
Keyword(s):  
Insulin Action ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Severely Obese ◽  
Differential Responses ◽  
Pulse Stimulation

scholarly journals Distinct Adaptations of Mitochondrial Dynamics to Electrical Pulse Stimulation in Lean and Severely Obese Primary Myotubes

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Benjamin A. Kugler ◽  
Wenqian Deng ◽  
Bergomi Francois ◽  
Meaghan Nasta ◽  
J. Matthew Hinkley ◽  
...  
Keyword(s):  
Mitochondrial Dynamics ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Severely Obese ◽  
Pulse Stimulation

scholarly journals Effects Of Electrical Pulse Stimulation On Mitochondrial Dynamics In Myotubes From Lean And Severely Obese Humans

2020 ◽  
Vol 52 (7S) ◽  
pp. 927-928
Author(s):  
Wenqian Deng ◽  
Benjamin A. Kugler ◽  
Bergomi Francois ◽  
Meaghan Nasta ◽  
Joseph Houmard ◽  
...  
Keyword(s):  
Mitochondrial Dynamics ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Severely Obese ◽  
Pulse Stimulation

scholarly journals Transcriptome Analyses of In Vitro Exercise Models by Clenbuterol Supplementation or Electrical Pulse Stimulation

2021 ◽  
Vol 11 (21) ◽  
pp. 10436
Author(s):  
Taku Fukushima ◽  
Miho Takata ◽  
Ayano Kato ◽  
Takayuki Uchida ◽  
Takeshi Nikawa ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Electrical Pulse ◽  
C2c12 Myotubes ◽  
Rna Seq ◽  
Gene Expressions ◽  
Beneficial Effects ◽  
Electrical Pulse Stimulation ◽  
Transcriptional Changes ◽  
Pulse Stimulation

Exercise has beneficial effects on human health and is affected by two different pathways; motoneuron and endocrine. For the advancement of exercise research, in vitro exercise models are essential. We established two in vitro exercise models using C2C12 myotubes; EPS (electrical pulse stimulation) for a motoneuron model and clenbuterol, a specific β2 adrenergic receptor agonist, treatment for an endocrine model. For clenbuterol treatment, we found that Ppargc1a was induced only in low glucose media (1 mg/mL) using a 1-h treatment of 30 ng/mL clenbuterol. Global transcriptional changes of clenbuterol treatment were analyzed by RNA-seq and gene ontology analyses and indicated that mitogenesis and the PI3K-Akt pathway were enhanced, which is consistent with the effects of exercise. Cxcl1 and Cxcl5 were identified as candidate myokines induced by adrenaline. As for the EPS model, we compared 1 Hz of 1-pulse EPS and 1 Hz of 10-pulse EPS for 24 h and determined Myh gene expressions. Ten-pulse EPS induced higher Myh2 and Myh7 expression. Global transcriptional changes of 10-pulse EPS were also analyzed using RNA-seq, and gene ontology analyses indicated that CaMK signaling and hypertrophy pathways were enhanced, which is also consistent with the effects of exercise. In this paper, we provided two transcriptome results of in vitro exercise models and these databases will contribute to advances in exercise research.

scholarly journals Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+,K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247377
Author(s):  
Vid Jan ◽  
Katarina Miš ◽  
Natasa Nikolic ◽  
Klemen Dolinar ◽  
Metka Petrič ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Human Skeletal Muscle ◽  
De Novo ◽  
Muscle Cells ◽  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Pulse Stimulation ◽  
Low Serum

Denervation reduces the abundance of Na+,K+-ATPase (NKA) in skeletal muscle, while reinnervation increases it. Primary human skeletal muscle cells, the most widely used model to study human skeletal muscle in vitro, are usually cultured as myoblasts or myotubes without neurons and typically do not contract spontaneously, which might affect their ability to express and regulate NKA. We determined how differentiation, de novo innervation, and electrical pulse stimulation affect expression of NKA (α and β) subunits and NKA regulators FXYD1 (phospholemman) and FXYD5 (dysadherin). Differentiation of myoblasts into myotubes under low serum conditions increased expression of myogenic markers CD56 (NCAM1), desmin, myosin heavy chains, dihydropyridine receptor subunit α1S, and SERCA2 as well as NKAα2 and FXYD1, while it decreased expression of FXYD5 mRNA. Myotubes, which were innervated de novo by motor neurons in co-culture with the embryonic rat spinal cord explants, started to contract spontaneously within 7–10 days. A short-term co-culture (10–11 days) promoted mRNA expression of myokines, such as IL-6, IL-7, IL-8, and IL-15, but did not affect mRNA expression of NKA, FXYDs, or myokines, such as musclin, cathepsin B, meteorin-like protein, or SPARC. A long-term co-culture (21 days) increased the protein abundance of NKAα1, NKAα2, FXYD1, and phospho-FXYD1Ser68 without attendant changes in mRNA levels. Suppression of neuromuscular transmission with α-bungarotoxin or tubocurarine for 24 h did not alter NKA or FXYD mRNA expression. Electrical pulse stimulation (48 h) of non-innervated myotubes promoted mRNA expression of NKAβ2, NKAβ3, FXYD1, and FXYD5. In conclusion, low serum concentration promotes NKAα2 and FXYD1 expression, while de novo innervation is not essential for upregulation of NKAα2 and FXYD1 mRNA in cultured myotubes. Finally, although innervation and EPS both stimulate contractions of myotubes, they exert distinct effects on the expression of NKA and FXYDs.

scholarly journals Reliable induction of sleep spindles with intracranial electrical pulse stimulation

2020 ◽  
Vol 28 (1) ◽  
pp. 7-11
Author(s):  
Michael J. Eckert ◽  
Kartik Iyer ◽  
David R. Euston ◽  
Masami Tatsuno
Keyword(s):  
Electrical Pulse ◽  
Sleep Spindles ◽  
Electrical Pulse Stimulation ◽  
Pulse Stimulation

Electrical Pulse Stimulation Induced Changes On Lipid, Mitochondrial, Glut4 And Ampk Content In Human Myotubes

2015 ◽  
Vol 47 ◽  
pp. 189
Author(s):  
Daniel Conde ◽  
Cecilia Gamboa ◽  
Jeffrey Covington ◽  
Arild Rustan ◽  
George A. King ◽  
...  
Keyword(s):  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Human Myotubes ◽  
Induced Changes ◽  
Pulse Stimulation

Control of myotube contraction using electrical pulse stimulation for bio-actuator

2009 ◽  
Vol 12 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Ken-ichi Yamasaki ◽  
Hiroyuki Hayashi ◽  
Keiko Nishiyama ◽  
Hiroyuki Kobayashi ◽  
Sadahito Uto ◽  
...  
Keyword(s):  
Electrical Pulse ◽  
Electrical Pulse Stimulation ◽  
Pulse Stimulation
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