scholarly journals Metabolic Changes in Single Human Muscle Fibres During Brief Maximal Exercise

2001 ◽  
Vol 86 (3) ◽  
pp. 411-415 ◽  
Author(s):  
C. Karatzaferi ◽  
A. de Haan ◽  
W. van Mechelen ◽  
A. J. Sargeant
Keyword(s):  
Maximal Exercise ◽  
Human Muscle ◽  
Metabolic Changes ◽  
Muscle Fibres

scholarly journals Denervation changes in normal and myasthenia gravis human muscle fibres during organ culture

1982 ◽  
Vol 333 (1) ◽  
pp. 227-249 ◽  
Author(s):  
S. G. Cull-Candy ◽  
R. Miledi ◽  
O. D. Uchitel
Keyword(s):  
Organ Culture ◽  
Myasthenia Gravis ◽  
Human Muscle ◽  
Muscle Fibres

Ultrastructural localization of dystropin in human muscle by using gold immunolabelling

1990 ◽  
Vol 240 (1297) ◽  
pp. 197-210 ◽  
Keyword(s):  
Plasma Membrane ◽  
Ultrastructural Localization ◽  
Ultrastructural Level ◽  
Human Muscle ◽  
Muscle Fibres ◽  
Mouse Muscle ◽  
Transverse Tubular System ◽  
Cytoplasmic Face ◽  
Tubular System ◽  
Gold Probe

Immunolabelling with a 5 nm gold probe was used to localize dystrophin at the ultrastructural level in human muscle. The primary antibody was monoclonal, raised against a segment (amino acids 1181-1388) from the rod domain of dystrophin. The antibody (Dy4/6D3) is specific for dystrophin and shows no immunoreactivity with any protein from mdx mouse muscle or from patients with a gene deletion spanning part of the molecule recognized by the antibody (Nicholson et al . 1989 a ; England et al . 1990). Using this antibody, labelling was almost entirely confined to a narrow 75 nm rim at the periphery of the muscle fibres. Histograms of the distance from the gold probe to the cytoplasmic face of the plasma membrane and of the distance between gold probes (nearest neighbour in a plane parallel with the plasma membrane) displayed modes at approximately 15 nm and 120 nm, respectively. The distribution of the probe was the same in longitudinal and transverse sections of the muscle. These observations suggest that the rod portion of the dystrophin mole­cule is normally arranged close to the cytoplasmic face of the plasma membrane and that the molecules form an interconnecting network. Labelling was not associated with the transverse tubular system.

P30 Labelling satellite cells on human muscle fibres

2012 ◽  
Vol 22 ◽  
pp. S15
Author(s):  
L. Boldrin ◽  
J.E. Morgan
Keyword(s):  
Satellite Cells ◽  
Human Muscle ◽  
Muscle Fibres
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