De novo neuromuscular junction formation on human muscle fibres cultured in monolayer and innervated by foetal rat spinal cord: Ultrastructural and ultrastructural-cytochemical studies

1987 ◽  
Vol 16 (4) ◽  
pp. 523-537 ◽  
Author(s):  
Valerie Askanas ◽  
Helen Kwan ◽  
Renate B. Alvarez ◽  
W. King Engel ◽  
Takayoshi Kobayashi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuromuscular Junction ◽  
De Novo ◽  
Human Muscle ◽  
Muscle Fibres ◽  
Rat Spinal Cord ◽  
Junction Formation ◽  
Foetal Rat

Histoenzymatic profile of human muscle cultured in monolayer and innervated de novo by fetal rat spinal cord

1988 ◽  
Vol 11 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Giuseppe Vita ◽  
Valerie Askanas ◽  
Andrea Martinuzzi ◽  
W. King Engel
Keyword(s):  
Spinal Cord ◽  
De Novo ◽  
Human Muscle ◽  
Rat Spinal Cord ◽  
Fetal Rat

An investigation of the foetal rat spinal cord

1975 ◽  
Vol 158 (2) ◽  
pp. 241-249 ◽  
Author(s):  
M. K. May ◽  
T. J. Biscoe
Keyword(s):  
Spinal Cord ◽  
Rat Spinal Cord ◽  
Foetal Rat

Neurotrophins increase motoneurons' ability to innervate skeletal muscle fibers in rat spinal cord-human muscle cocultures

1996 ◽  
Vol 136 (1-2) ◽  
pp. 17-23 ◽  
Author(s):  
Serge Braun ◽  
Bernard Croizat ◽  
Marie-Claude Lagrange ◽  
Jean-Marie Warter ◽  
Philippe Poindron
Keyword(s):  
Skeletal Muscle ◽  
Spinal Cord ◽  
Muscle Fibers ◽  
Human Muscle ◽  
Rat Spinal Cord ◽  
Skeletal Muscle Fibers

Differentiation of glial cells and motor neurons during the formation of neuromuscular junctions in cocultures of rat spinal cord explant and human muscle

2001 ◽  
Vol 438 (2) ◽  
pp. 239-251 ◽  
Author(s):  
Tomaz Mars ◽  
Kevin J. Yu ◽  
Xue-Ming Tang ◽  
Armand F. Miranda ◽  
Zoran Grubic ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glial Cells ◽  
Motor Neurons ◽  
Neuromuscular Junctions ◽  
Human Muscle ◽  
Rat Spinal Cord

Developmental study of the expression of dystrophin in cultured human muscle aneurally and innervated with fetal rat spinal cord

1991 ◽  
Vol 565 (2) ◽  
pp. 280-289 ◽  
Author(s):  
Yong Choo Park-Matsumoto ◽  
Noriyoshi Kameda ◽  
Takayoshi Kobayashi ◽  
Hiroshi Tsukagoshi
Keyword(s):  
Spinal Cord ◽  
Human Muscle ◽  
Developmental Study ◽  
Rat Spinal Cord ◽  
Fetal Rat

scholarly journals Expression of muscle-gene-specific isozymes of phosphorylase and creatine kinase in innervated cultured human muscle.

1986 ◽  
Vol 103 (4) ◽  
pp. 1423-1429 ◽  
Author(s):  
A Martinuzzi ◽  
V Askanas ◽  
T Kobayashi ◽  
W K Engel ◽  
S Di Mauro
Keyword(s):  
Spinal Cord ◽  
Creatine Kinase ◽  
Glycogen Phosphorylase ◽  
De Novo ◽  
Muscle Fibers ◽  
Total Activity ◽  
Human Muscle ◽  
Rat Embryo ◽  
Muscle Gene ◽  
Large Groups

Isozymes of creatine kinase and glycogen phosphorylase are excellent markers of skeletal muscle maturation. In adult innervated muscle only the muscle-gene-specific isozymes are present, whereas aneurally cultured human muscle has predominantly the fetal pattern of isozymes. We have studied the isozyme pattern of human muscle cultured in monolayer and innervated by rat embryo spinal cord explants for 20-42 d. In this culture system, large groups of innervated muscle fibers close to the ventral part of the spinal cord explant continuously contracted. The contractions were reversibly blocked by 1 mM d-tubocurarine. In those innervated fibers, the total activity and the muscle-gene-specific isozymes of both enzymes increased significantly. The amount of muscle-gene-specific isozymes directly correlated with the duration of innervation. Control noninnervated muscle fibers from the same dishes as the innervated fibers remained biochemically immature. This study demonstrated that de novo innervation of human muscle cultured in monolayer exerts a time-related maturational influence that is not mediated by a diffusable neural factor.

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